FastQCFastQC Report
Thu 2 Feb 2017
SRR4062007_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062007_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447255
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22090.15263377911978193No Hit
GCGCAAGACGGACCAGAGCGAAAGC17890.12361332315314164No Hit
GGGTAGGCACACGCTGAGCCAGTCA15390.10633924222061765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA551.1288221E-515.54454916
TTAGATA508.707316E-515.2012194
ACGTCGT456.7542715E-414.7799858
GGTATCA5500.014.1569351
ACCGTTA759.655087E-713.9354148
AAGACGG3150.013.2713635
CAAGACG3300.013.2434854
ACGCGCG655.4478776E-513.15490113
CGCGGTT2350.012.93810310
CGGTCCA2450.012.7978310
TCGCGTA1207.366907E-1012.6698739
GTATCAA16200.012.3676011
TAGCGAA1001.43933E-712.35184510
CGGTTCT2400.012.27181712
TGGACTA701.089631E-412.2156875
ATAGGAC3500.012.2127293
AACGTCG550.003064836612.0927157
ACTGCGC550.003064836612.0927158
CGAAATT1103.8082362E-812.09187913
GGGCGTA550.003069605412.09020416
Produced by FastQC (version 0.11.5)