##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062007_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447255
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331949794611177	32.0	32.0	32.0	32.0	32.0
2	30.910573464938796	32.0	32.0	32.0	32.0	32.0
3	30.933752517697297	32.0	32.0	32.0	32.0	32.0
4	30.94648489727104	32.0	32.0	32.0	32.0	32.0
5	30.879221699009506	32.0	32.0	32.0	32.0	32.0
6	34.55085869456315	36.0	36.0	36.0	32.0	36.0
7	34.49146694950095	36.0	36.0	36.0	32.0	36.0
8	34.45679026847377	36.0	36.0	36.0	32.0	36.0
9	34.57487864958145	36.0	36.0	36.0	32.0	36.0
10	34.29683297000183	36.0	36.0	36.0	32.0	36.0
11	34.556511464807514	36.0	36.0	36.0	32.0	36.0
12	34.39312698867857	36.0	36.0	36.0	32.0	36.0
13	34.46542178123413	36.0	36.0	36.0	32.0	36.0
14	34.382290612227976	36.0	36.0	36.0	32.0	36.0
15	34.329833374215326	36.0	36.0	36.0	32.0	36.0
16	34.330462841724504	36.0	36.0	36.0	32.0	36.0
17	34.27660502122985	36.0	36.0	36.0	32.0	36.0
18	34.27975926840812	36.0	36.0	36.0	32.0	36.0
19	34.29183108712701	36.0	36.0	36.0	32.0	36.0
20	34.27315780563895	36.0	36.0	36.0	32.0	36.0
21	34.252304535137206	36.0	36.0	36.0	32.0	36.0
22	34.23565370304473	36.0	36.0	36.0	32.0	36.0
23	34.201323885562665	36.0	36.0	36.0	32.0	36.0
24	34.17211410566901	36.0	36.0	36.0	32.0	36.0
25	33.84269323650635	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	17.0
5	110.0
6	317.0
7	65.0
8	267.0
9	209.0
10	119.0
11	26.0
12	56.0
13	70.0
14	208.0
15	322.0
16	492.0
17	631.0
18	835.0
19	1107.0
20	1659.0
21	2473.0
22	3650.0
23	5303.0
24	7675.0
25	10901.0
26	14772.0
27	19126.0
28	25530.0
29	33990.0
30	44747.0
31	61382.0
32	87978.0
33	127299.0
34	289331.0
35	706587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53570935021144	17.180149802382466	11.63614880738509	25.647992040021006
2	16.99903195916678	18.791738555732827	37.450121022378255	26.759108462722136
3	19.99408532608095	21.737324909068796	27.492731031585464	30.775858733264787
4	13.20647411817712	14.962933171908817	34.36227641737082	37.468316292543236
5	15.50927589925074	35.55855843401808	32.85541485802759	16.076750808703586
6	35.814440687809835	34.1183547323225	16.00430053998742	14.062904039880248
7	31.75959282378201	29.55472364256148	19.041008145737877	19.64467538791863
8	28.6607121857953	32.94941873254226	18.30103608520552	20.08883299645692
9	26.654592221114555	15.166827282620202	18.500605682414868	39.677974813850376
10	17.04009780729279	26.289515398629064	30.023573107422656	26.64681368665549
11	37.52592314777076	21.479555225559928	21.25867651302264	19.735845113646665
12	24.774490844366337	23.50768187589299	27.81818118917722	23.899646090563454
13	29.28135551004594	19.45881994797144	24.8899928045608	26.369831737421816
14	23.55537845628934	19.670470841920228	24.378751685383154	32.395399016407275
15	25.369123244506657	27.09643805323512	21.715773544904717	25.81866515735351
16	26.184478787824283	26.067838283405987	22.898966216454614	24.84871671231512
17	24.171981088085413	26.269753527996357	24.473481394566907	25.084783989351322
18	24.945697138066237	25.2219153899153	25.511830414583326	24.320557057435135
19	25.7212201864877	25.008663149762167	24.88423196076302	24.38588470298711
20	25.62982492298797	24.389540472985967	24.798980273577715	25.18165433044835
21	26.656103201960903	24.06602557363625	24.46793059374879	24.809940630654058
22	25.922521221377426	24.321441614927455	24.774298062131322	24.981739101563797
23	24.64376381809116	24.580279262887604	25.336215725774387	25.439741193246846
24	24.761735478659244	25.023100246771495	24.983193533037504	25.231970741531757
25	24.796218862255984	24.5260783144779	25.057506878002428	25.620195945263685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	271.0
3	410.0
4	410.0
5	410.0
6	885.0
7	1360.0
8	1360.0
9	1360.0
10	1690.5
11	2021.0
12	2021.0
13	2021.0
14	2759.5
15	3498.0
16	3498.0
17	3498.0
18	5836.5
19	8175.0
20	8175.0
21	8175.0
22	12681.5
23	17188.0
24	17188.0
25	17188.0
26	25364.5
27	33541.0
28	33541.0
29	33541.0
30	43319.5
31	53098.0
32	53098.0
33	53098.0
34	67622.5
35	82147.0
36	82147.0
37	82147.0
38	97673.0
39	113199.0
40	113199.0
41	113199.0
42	132194.0
43	151189.0
44	151189.0
45	151189.0
46	167630.0
47	184071.0
48	184071.0
49	184071.0
50	192498.0
51	200925.0
52	200925.0
53	200925.0
54	190436.0
55	179947.0
56	179947.0
57	179947.0
58	165693.5
59	151440.0
60	151440.0
61	151440.0
62	133186.5
63	114933.0
64	114933.0
65	114933.0
66	94364.5
67	73796.0
68	73796.0
69	73796.0
70	55829.0
71	37862.0
72	37862.0
73	37862.0
74	28551.0
75	19240.0
76	19240.0
77	19240.0
78	15250.0
79	11260.0
80	11260.0
81	11260.0
82	7829.0
83	4398.0
84	4398.0
85	4398.0
86	3230.0
87	2062.0
88	2062.0
89	2062.0
90	1347.5
91	633.0
92	633.0
93	633.0
94	389.5
95	146.0
96	146.0
97	146.0
98	365.0
99	584.0
100	584.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010364448559514391
2	1.3819264746019186E-4
3	4.8367426611067155E-4
4	0.009604388998483336
5	0.03420268024639749
6	0.06391409945033874
7	0.10993225105458264
8	0.11200514076648552
9	0.12285326359211057
10	0.13570517980590843
11	0.14634601366034322
12	0.136119757748289
13	0.13093753346853182
14	0.12008941064290675
15	0.13860722540257248
16	0.12354422682941155
17	0.12554802021758432
18	0.11463080106822916
19	0.10198617382562161
20	0.0951456377763421
21	0.09611298630856345
22	0.10661562751553805
23	0.0852648634829384
24	0.09556021571872268
25	0.09265817012205865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1447255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.629512542712234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34692530264498	34.69566198888926
2	15.40732900453322	14.985017981512843
3	5.680706786928778	8.287500058392707
4	2.5988538779585943	5.055239890194551
5	1.4491130640206091	3.523483096129918
6	0.8181988609763127	2.3873167063368275
7	0.52966577946309	1.803017206609205
8	0.3784614509664911	1.472351670136644
9	0.26243859433698663	1.1486034823502034
>10	1.3338288604075585	11.531931608056414
>50	0.10185698509798453	3.4180479893463267
>100	0.08405864634193176	8.12831122163982
>500	0.0062793766372935865	2.0936105394263804
>1k	0.0022834096862885764	1.4699065609789084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2209	0.15263377911978193	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1789	0.12361332315314164	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1539	0.10633924222061765	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.909632373009594E-5
2	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-5
3	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-5
4	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-5
5	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-5
6	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-5
7	0.0	0.0	0.0	1.3819264746019189E-4	6.909632373009594E-5
8	0.0	0.0	0.0	1.3819264746019189E-4	6.909632373009594E-5
9	0.0	0.0	0.0	2.0728897119028783E-4	6.909632373009594E-5
10	0.0	0.0	0.0	2.0728897119028783E-4	6.909632373009594E-5
11	0.0	0.0	0.0	2.0728897119028783E-4	6.909632373009594E-5
12	0.0	0.0	0.0	2.0728897119028783E-4	3.454816186504797E-4
13	0.0	0.0	0.0	2.7638529492038377E-4	5.527705898407675E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	55	1.1288221E-5	15.544549	16
TTAGATA	50	8.707316E-5	15.201219	4
ACGTCGT	45	6.7542715E-4	14.779985	8
GGTATCA	550	0.0	14.156935	1
ACCGTTA	75	9.655087E-7	13.935414	8
AAGACGG	315	0.0	13.271363	5
CAAGACG	330	0.0	13.243485	4
ACGCGCG	65	5.4478776E-5	13.154901	13
CGCGGTT	235	0.0	12.938103	10
CGGTCCA	245	0.0	12.79783	10
TCGCGTA	120	7.366907E-10	12.669873	9
GTATCAA	1620	0.0	12.367601	1
TAGCGAA	100	1.43933E-7	12.351845	10
CGGTTCT	240	0.0	12.271817	12
TGGACTA	70	1.089631E-4	12.215687	5
ATAGGAC	350	0.0	12.212729	3
AACGTCG	55	0.0030648366	12.092715	7
ACTGCGC	55	0.0030648366	12.092715	8
CGAAATT	110	3.8082362E-8	12.091879	13
GGGCGTA	55	0.0030696054	12.090204	16
>>END_MODULE