FastQCFastQC Report
Thu 2 Feb 2017
SRR4062007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447255
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18550.12817368051932798No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14640.10115701794086046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.032.104471
TAACGTT407.0330326E-427.4998475
GTATCAA17100.026.9008241
ACGACGT1107.2759576E-1225.9989596
CGACGTT1407.2759576E-1221.9991197
ACCGTTA956.8708687E-720.841278
CGCTCGA654.9329933E-420.3075810
TCGCGTG1658.185452E-1118.66656525
ATACCGT3650.018.0814676
ATTCTAT1852.1827873E-1117.8457551
GTGTAGG2100.017.8174291
CTCGCGT1802.8558134E-1017.11101724
TACCGTC3650.016.8760367
CGCAATA2500.016.71990836
ATCAACG27550.016.5298563
ACCGTCG3750.016.4260088
TCAACGC27850.016.3517954
CACGACG1753.6270649E-916.3427685
TATCAAC28500.016.1354752
GCGCAAT2600.016.07627935
Produced by FastQC (version 0.11.5)