##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062007_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447255
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28007503860757	32.0	32.0	32.0	32.0	32.0
2	31.490999858352538	32.0	32.0	32.0	32.0	32.0
3	31.600715146950606	32.0	32.0	32.0	32.0	32.0
4	31.675980390463327	32.0	32.0	32.0	32.0	32.0
5	31.65489702920356	32.0	32.0	32.0	32.0	32.0
6	35.31803932271784	36.0	36.0	36.0	36.0	36.0
7	35.32284082625384	36.0	36.0	36.0	36.0	36.0
8	35.27539859941752	36.0	36.0	36.0	36.0	36.0
9	35.348458288276774	36.0	36.0	36.0	36.0	36.0
10	35.24463415223993	36.0	36.0	36.0	36.0	36.0
11	35.35447243229424	36.0	36.0	36.0	36.0	36.0
12	35.276133784302004	36.0	36.0	36.0	36.0	36.0
13	35.31274930817306	36.0	36.0	36.0	36.0	36.0
14	35.27397037840602	36.0	36.0	36.0	36.0	36.0
15	35.25654290363481	36.0	36.0	36.0	36.0	36.0
16	35.26547153058721	36.0	36.0	36.0	36.0	36.0
17	35.25188373852569	36.0	36.0	36.0	36.0	36.0
18	35.245406649139234	36.0	36.0	36.0	36.0	36.0
19	35.23739009366007	36.0	36.0	36.0	36.0	36.0
20	35.234686354512505	36.0	36.0	36.0	36.0	36.0
21	35.22751691996228	36.0	36.0	36.0	36.0	36.0
22	35.21338948561242	36.0	36.0	36.0	36.0	36.0
23	35.18503719109625	36.0	36.0	36.0	36.0	36.0
24	35.17128287689454	36.0	36.0	36.0	36.0	36.0
25	35.15874120317429	36.0	36.0	36.0	36.0	36.0
26	35.12133245350681	36.0	36.0	36.0	36.0	36.0
27	35.102751070129315	36.0	36.0	36.0	36.0	36.0
28	35.08285823852742	36.0	36.0	36.0	36.0	36.0
29	35.07168536298026	36.0	36.0	36.0	36.0	36.0
30	35.045346535337586	36.0	36.0	36.0	36.0	36.0
31	35.05386887590646	36.0	36.0	36.0	36.0	36.0
32	35.02307402634643	36.0	36.0	36.0	36.0	36.0
33	35.008593509782315	36.0	36.0	36.0	36.0	36.0
34	35.00393710852614	36.0	36.0	36.0	36.0	36.0
35	34.98437455735168	36.0	36.0	36.0	36.0	36.0
36	34.96177867756546	36.0	36.0	36.0	36.0	36.0
37	34.95410898563142	36.0	36.0	36.0	36.0	36.0
38	34.94397393686669	36.0	36.0	36.0	36.0	36.0
39	34.92313033985027	36.0	36.0	36.0	36.0	36.0
40	34.910384831975016	36.0	36.0	36.0	36.0	36.0
41	34.8891764063693	36.0	36.0	36.0	36.0	36.0
42	34.855689564036744	36.0	36.0	36.0	32.0	36.0
43	34.851975636636254	36.0	36.0	36.0	32.0	36.0
44	34.807909456177384	36.0	36.0	36.0	32.0	36.0
45	34.798281574428835	36.0	36.0	36.0	32.0	36.0
46	34.77739582865494	36.0	36.0	36.0	32.0	36.0
47	34.76014800432543	36.0	36.0	36.0	32.0	36.0
48	34.73547508904789	36.0	36.0	36.0	32.0	36.0
49	34.719516602119185	36.0	36.0	36.0	32.0	36.0
50	34.3252481421726	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	32.0
22	120.0
23	292.0
24	698.0
25	1679.0
26	3378.0
27	6218.0
28	10518.0
29	16680.0
30	24426.0
31	36273.0
32	54842.0
33	94161.0
34	219119.0
35	978805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03620137185892	17.66157772070147	12.06458961733375	26.237631290105863
2	16.475927632951855	19.155763838311525	37.11547577423095	27.25283275450567
3	19.407579483558116	22.15147497078941	27.707947975780257	30.732997569872218
4	12.988675787854644	15.328073253259797	34.57723010420431	37.10602085468125
5	15.218013172550462	35.79590838361253	32.74315737687701	16.242921066960005
6	36.01420345012002	34.16635918781361	15.864734179349306	13.954703182717063
7	31.799648299712214	29.523269914424205	19.10769007534954	19.569391710514044
8	28.634622094931437	33.035021471682604	18.272799195718793	20.05755723766717
9	26.775101830707097	14.90172775357487	18.52520806630483	39.797962349413204
10	17.036596867863647	26.284241546928495	30.057591785829036	26.621569799378825
11	37.693357424918204	21.31925610897872	21.28056216768987	19.706824298413203
12	24.84759078393234	23.439753187931636	27.79157784910054	23.921078179035486
13	29.37713118973505	19.35236015767781	24.925462340776157	26.345046311810982
14	23.64414011352526	19.48923997498713	24.410971114281864	32.45564879720575
15	25.40043047009684	27.04236641089511	21.72250225426756	25.83470086474049
16	26.333892785553836	25.878131544744548	22.896612815071908	24.891362854629715
17	24.32121498975647	26.148190885503936	24.45643649529627	25.074157629443327
18	24.965555458820635	25.181343426931278	25.567730336209127	24.28537077803896
19	25.779631094727606	24.87446925386335	24.939419798169638	24.406479853239407
20	25.761788548227955	24.204699665296715	24.867265686970892	25.16624609950444
21	26.767224849801867	23.979257283616224	24.47495431005593	24.778563556525977
22	26.027825089566107	24.194768717330394	24.945638467305347	24.831767725798148
23	24.732009723241394	24.41962502781129	25.394505732925943	25.453859516021378
24	24.80160526132137	24.93627529899996	25.033840251899996	25.22827918777868
25	25.025824786682076	24.320350346483995	25.14480882057249	25.509016046261436
26	24.85367085598835	25.267282592998725	25.194109383928563	24.684937167084357
27	25.412730756447914	24.549162515693546	24.96560720980673	25.07249951805181
28	25.07275958519436	24.494759651527882	25.330492968646357	25.10198779463141
29	24.665951745591283	24.840904725250578	25.36451992266691	25.128623606491225
30	24.711312567369614	25.194646361349882	25.44270473452917	24.651336336751335
31	25.4370878819056	24.536551761307145	24.849835791905885	25.17652456488137
32	25.20243635320903	24.67971905240647	24.55693403673877	25.560910557645734
33	24.76358676500462	24.58752921049104	25.02290544714335	25.625978577360986
34	25.321697432017388	24.722559069127893	25.111643156480074	24.84410034237465
35	25.61708284591568	24.334376919584898	25.474813318749383	24.573726915750044
36	24.604232437911598	24.97921922320316	25.036016558288782	25.380531780596456
37	25.517261870213286	24.57449925236796	24.980445483666795	24.92779339375195
38	24.86610859869201	24.397911909096877	25.326290114734444	25.409689377476674
39	25.60492354805566	24.599844256240633	24.507945764688728	25.287286431014984
40	25.49144794210277	24.78770139864875	25.017308641054026	24.703542018194458
41	24.81822333913517	25.050734396366053	25.43083642484365	24.700205839655123
42	25.86757545982087	25.069824778156903	24.728336352969954	24.334263409052276
43	25.55443231451609	24.093819725110748	25.033841187229694	25.317906773143466
44	24.68039942039681	24.8332466139717	24.921693452930043	25.564660512701447
45	25.17965528297089	24.88329316425468	24.985074791876265	24.951976760898166
46	25.09587333525886	24.716249476583414	24.873239903760446	25.31463728439728
47	24.896458228824205	24.767937912249643	25.26778520800253	25.067818650923616
48	25.462716640386283	25.171812876326516	24.45229247367701	24.91317800961019
49	24.89712972054524	25.169515709872297	24.57499286558767	25.358361703994785
50	25.074521260497583	25.170358987257107	24.78825182865569	24.966867923589625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	61.5
2	66.0
3	158.0
4	250.0
5	253.0
6	256.0
7	203.5
8	151.0
9	197.0
10	243.0
11	458.5
12	674.0
13	1223.0
14	1772.0
15	2212.5
16	2653.0
17	2570.0
18	2487.0
19	2447.5
20	2408.0
21	2842.5
22	3277.0
23	4003.0
24	4729.0
25	6055.0
26	7381.0
27	9078.5
28	10776.0
29	12927.5
30	15079.0
31	17684.5
32	20290.0
33	23710.5
34	27131.0
35	31547.0
36	35963.0
37	42354.5
38	48746.0
39	54640.0
40	60534.0
41	66128.0
42	71722.0
43	75763.5
44	79805.0
45	86490.0
46	93175.0
47	99392.0
48	105609.0
49	110264.5
50	114920.0
51	114305.5
52	113691.0
53	112953.0
54	112215.0
55	111726.0
56	111237.0
57	105321.5
58	99406.0
59	93125.5
60	86845.0
61	76191.5
62	65538.0
63	56106.0
64	46674.0
65	40120.0
66	33566.0
67	28507.5
68	23449.0
69	20414.0
70	17379.0
71	13497.0
72	9615.0
73	8451.5
74	7288.0
75	5337.0
76	3386.0
77	2980.0
78	2574.0
79	2195.5
80	1817.0
81	1487.0
82	1157.0
83	929.5
84	702.0
85	524.0
86	346.0
87	227.5
88	109.0
89	77.0
90	45.0
91	31.5
92	18.0
93	15.0
94	12.0
95	12.0
96	12.0
97	13.0
98	14.0
99	10.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03171521259211404
2	0.005320416927217388
3	4.1457794238057566E-4
4	1.3819264746019186E-4
5	2.0728897119028783E-4
6	6.909632373009593E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.1457794238057566E-4
17	0.0
18	6.909632373009593E-5
19	0.0
20	2.0728897119028783E-4
21	0.0
22	0.0
23	6.909632373009593E-5
24	9.673485322213431E-4
25	1.3819264746019186E-4
26	2.763852949203837E-4
27	2.763852949203837E-4
28	0.0015892154457922065
29	6.218669135708635E-4
30	0.0010364448559514391
31	4.1457794238057566E-4
32	6.909632373009594E-4
33	6.909632373009593E-5
34	6.909632373009594E-4
35	1.3819264746019186E-4
36	2.763852949203837E-4
37	0.001312830150871823
38	0.0
39	4.1457794238057566E-4
40	6.909632373009593E-5
41	8.291558847611513E-4
42	0.0042148757475358525
43	0.0037312014814251805
44	0.004007586776345565
45	0.002694756625473742
46	0.002280178683093166
47	8.982522084912473E-4
48	0.0028329492729339332
49	0.0030402382441242215
50	0.001174637503411631
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1447255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.778913579020525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79120405899864	35.23909229007701
2	15.84169818164232	15.77165049457799
3	5.891746692022782	8.798542482350463
4	2.6584452869435604	5.293380727732718
5	1.4232772073395497	3.542459655157256
6	0.8163806140398214	2.4383124020325586
7	0.5377769903909113	1.8738968030618444
8	0.3569304796299652	1.4214089199374709
9	0.258380969194756	1.1575731542408394
>10	1.2534599575626182	11.111368125802832
>50	0.09294123786979408	3.2462923269938453
>100	0.06952186725005227	6.871246713791034
>500	0.006282043562476936	2.0397246415900274
>1k	0.001954413552770602	1.195051262654119
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1855	0.12817368051932798	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1464	0.10115701794086046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0728897119028783E-4
2	0.0	0.0	0.0	0.0	2.0728897119028783E-4
3	0.0	0.0	0.0	0.0	2.0728897119028783E-4
4	0.0	0.0	0.0	0.0	2.0728897119028783E-4
5	0.0	0.0	0.0	0.0	2.7638529492038377E-4
6	0.0	0.0	0.0	0.0	2.7638529492038377E-4
7	0.0	0.0	0.0	0.0	2.7638529492038377E-4
8	0.0	0.0	0.0	0.0	2.7638529492038377E-4
9	0.0	0.0	0.0	0.0	2.7638529492038377E-4
10	0.0	0.0	0.0	6.909632373009594E-5	2.7638529492038377E-4
11	0.0	0.0	0.0	6.909632373009594E-5	2.7638529492038377E-4
12	0.0	0.0	0.0	6.909632373009594E-5	6.909632373009594E-4
13	0.0	0.0	0.0	1.3819264746019189E-4	8.291558847611513E-4
14	0.0	0.0	0.0	1.3819264746019189E-4	8.982522084912473E-4
15	0.0	0.0	0.0	2.0728897119028783E-4	0.001105541179681535
16	0.0	0.0	0.0	4.1457794238057566E-4	0.001174637503411631
17	0.0	0.0	0.0	4.836742661106716E-4	0.001174637503411631
18	0.0	0.0	0.0	7.600595610310554E-4	0.001243733827141727
19	0.0	0.0	0.0	9.673485322213432E-4	0.001243733827141727
20	0.0	0.0	0.0	0.001174637503411631	0.0013819264746019189
21	0.0	0.0	0.0	0.0017965044169824945	0.0014510227983320148
22	0.0	0.0	0.0	0.0020728897119028783	0.0014510227983320148
23	0.0	0.0	0.0	0.002694756625473742	0.0015201191220621108
24	0.0	0.0	0.0	0.003938490452615469	0.0015201191220621108
25	0.0	0.0	0.0	0.004836742661106716	0.0015892154457922067
26	0.0	0.0	0.0	0.006011380164518347	0.0015892154457922067
27	0.0	0.0	0.0	0.00732421031539017	0.0017274080932523986
28	0.0	0.0	0.0	0.009466196351023144	0.0017274080932523986
29	0.0	0.0	0.0	0.012230049300226982	0.0017274080932523986
30	0.0	0.0	0.0	0.016583117695223026	0.0017274080932523986
31	0.0	0.0	0.0	0.025980217722516075	0.0017965044169824945
32	0.0	0.0	0.0	0.03662105157695085	0.0017965044169824945
33	0.0	0.0	0.0	0.046432729546624474	0.0017965044169824945
34	0.0	0.0	0.0	0.05776452663836021	0.0018656007407125905
35	0.0	0.0	0.0	0.07034005755723767	0.0018656007407125905
36	0.0	0.0	0.0	0.08485028554055782	0.0018656007407125905
37	0.0	0.0	0.0	0.11083050326307389	0.0018656007407125905
38	0.0	0.0	0.0	0.14330577541621897	0.0018656007407125905
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	665	0.0	32.10447	1
TAACGTT	40	7.0330326E-4	27.499847	5
GTATCAA	1710	0.0	26.900824	1
ACGACGT	110	7.2759576E-12	25.998959	6
CGACGTT	140	7.2759576E-12	21.999119	7
ACCGTTA	95	6.8708687E-7	20.84127	8
CGCTCGA	65	4.9329933E-4	20.30758	10
TCGCGTG	165	8.185452E-11	18.666565	25
ATACCGT	365	0.0	18.081467	6
ATTCTAT	185	2.1827873E-11	17.845755	1
GTGTAGG	210	0.0	17.817429	1
CTCGCGT	180	2.8558134E-10	17.111017	24
TACCGTC	365	0.0	16.876036	7
CGCAATA	250	0.0	16.719908	36
ATCAACG	2755	0.0	16.529856	3
ACCGTCG	375	0.0	16.426008	8
TCAACGC	2785	0.0	16.351795	4
CACGACG	175	3.6270649E-9	16.342768	5
TATCAAC	2850	0.0	16.135475	2
GCGCAAT	260	0.0	16.076279	35
>>END_MODULE