FastQCFastQC Report
Thu 2 Feb 2017
SRR4062006_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062006_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851286
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37440.1313091706689543No Hit
GCGCAAGACGGACCAGAGCGAAAGC37050.12994136680781937No Hit
GGGTAGGCACACGCTGAGCCAGTCA30390.1065834854868996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.014.8430721
AAGACGG6400.012.9154835
CAAGACG6500.012.8618244
CGTCGTA4500.012.24643610
CGGACAT3200.012.1732145
CGCAAGA6350.012.1166762
CGGACCA7000.011.9443829
GTATCAA30150.011.9373991
CGGTTCT5900.011.91593712
GCCGTTA658.030255E-411.69174716
GCAAGAC7750.011.522233
GACGGAC6850.011.5126217
TACCGTC4800.011.4808347
ACCGTCG5000.011.4016558
CGCATCG3950.011.30444613
GTTTTCG3200.011.281301515
TTTTCGG3300.011.22673316
CCGTCGT4850.011.1663739
AATCGTA600.00587518511.08396913
GTATACG600.00589441611.0791031
Produced by FastQC (version 0.11.5)