FastQCFastQC Report
Thu 2 Feb 2017
SRR4062006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851286
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT34600.1213487528083819No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28970.1016032765566134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32200.026.6545351
GGTATCA15850.023.7426531
GTCCTAA4750.019.458891
CGAGCCG8500.017.59917815
TCAACGC48150.017.4986654
ATCAACG48650.017.409263
TAGGACG4950.017.3325254
CGGATCG2450.017.06132726
AACGCAG49850.016.9460496
ACCGTCG7100.016.7316158
CGTCGTA7550.016.60849810
CAACGCA50850.016.526275
TATCAAC52050.016.5262262
ATACCGT8350.016.0711086
GTAGGAC9550.015.8945483
TACCGTC7800.015.7941357
GTCGATA850.002981498615.52923336
TCTAGAT7700.015.4283922
CGTCTTA2001.2805685E-915.399281515
CGCAATA4150.015.37331536
Produced by FastQC (version 0.11.5)