##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2851286
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30246422140746	32.0	32.0	32.0	32.0	32.0
2	31.484904706157153	32.0	32.0	32.0	32.0	32.0
3	31.592730087406174	32.0	32.0	32.0	32.0	32.0
4	31.668265828121065	32.0	32.0	32.0	32.0	32.0
5	31.647459076360633	32.0	32.0	32.0	32.0	32.0
6	35.303760829324034	36.0	36.0	36.0	36.0	36.0
7	35.30866914087187	36.0	36.0	36.0	36.0	36.0
8	35.26481349117556	36.0	36.0	36.0	36.0	36.0
9	35.3422140044878	36.0	36.0	36.0	36.0	36.0
10	35.22695478461298	36.0	36.0	36.0	36.0	36.0
11	35.338994053911115	36.0	36.0	36.0	36.0	36.0
12	35.26319492327322	36.0	36.0	36.0	36.0	36.0
13	35.301631614646865	36.0	36.0	36.0	36.0	36.0
14	35.26685748115061	36.0	36.0	36.0	36.0	36.0
15	35.246434065190236	36.0	36.0	36.0	36.0	36.0
16	35.25294200581772	36.0	36.0	36.0	36.0	36.0
17	35.236897315807674	36.0	36.0	36.0	36.0	36.0
18	35.23583077951493	36.0	36.0	36.0	36.0	36.0
19	35.22615163824324	36.0	36.0	36.0	36.0	36.0
20	35.226619848026466	36.0	36.0	36.0	36.0	36.0
21	35.21372391264854	36.0	36.0	36.0	36.0	36.0
22	35.19954224164114	36.0	36.0	36.0	36.0	36.0
23	35.17072822579005	36.0	36.0	36.0	36.0	36.0
24	35.15558348057684	36.0	36.0	36.0	36.0	36.0
25	35.14320134844417	36.0	36.0	36.0	36.0	36.0
26	35.10283780722102	36.0	36.0	36.0	36.0	36.0
27	35.086487290296375	36.0	36.0	36.0	36.0	36.0
28	35.070001395861375	36.0	36.0	36.0	36.0	36.0
29	35.0535239186809	36.0	36.0	36.0	36.0	36.0
30	35.036781297982735	36.0	36.0	36.0	36.0	36.0
31	35.037342799003675	36.0	36.0	36.0	36.0	36.0
32	35.01359316462817	36.0	36.0	36.0	36.0	36.0
33	34.99897309494733	36.0	36.0	36.0	36.0	36.0
34	34.98656360673745	36.0	36.0	36.0	36.0	36.0
35	34.974658452361496	36.0	36.0	36.0	36.0	36.0
36	34.95353640427513	36.0	36.0	36.0	36.0	36.0
37	34.94041635949533	36.0	36.0	36.0	36.0	36.0
38	34.92506679442189	36.0	36.0	36.0	36.0	36.0
39	34.91125793764638	36.0	36.0	36.0	36.0	36.0
40	34.90131575717062	36.0	36.0	36.0	36.0	36.0
41	34.874514166590096	36.0	36.0	36.0	32.0	36.0
42	34.84579133766307	36.0	36.0	36.0	32.0	36.0
43	34.84716475302723	36.0	36.0	36.0	32.0	36.0
44	34.798956681301	36.0	36.0	36.0	32.0	36.0
45	34.795044411539216	36.0	36.0	36.0	32.0	36.0
46	34.77243531515253	36.0	36.0	36.0	32.0	36.0
47	34.748683927182334	36.0	36.0	36.0	32.0	36.0
48	34.723481264243574	36.0	36.0	36.0	32.0	36.0
49	34.70857676150341	36.0	36.0	36.0	32.0	36.0
50	34.321598394548985	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	26.0
21	65.0
22	206.0
23	524.0
24	1346.0
25	3106.0
26	6633.0
27	12433.0
28	20873.0
29	33339.0
30	49029.0
31	72531.0
32	110271.0
33	187693.0
34	439372.0
35	1913834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.542424614521025	17.30941814163173	11.786061360885505	26.36209588296174
2	16.606720536930265	19.136733643825643	36.75398634455808	27.502559474686016
3	19.32319472180545	22.147542259997095	27.80595227824155	30.723310739955906
4	13.077432428735666	15.282577756142315	34.105557983309986	37.53443183181203
5	15.332244935178348	35.780393752290635	32.69024317106147	16.19711814146955
6	36.391725821385485	33.6986195673522	15.869539294632586	14.040115316629725
7	32.009416102067625	29.34139191929536	19.172506721528464	19.476685257108546
8	28.90471176865457	33.04031935063687	18.151458675138166	19.9035102055704
9	27.011075002647928	14.449024054409133	18.233737338169515	40.306163604773424
10	17.191652184892074	26.139231960326658	30.05771082161201	26.611405033169255
11	37.95897710717199	20.85076698724716	21.260862642330515	19.929393263250336
12	25.064444604995778	23.0890903262598	27.710864501140886	24.13560056760353
13	29.890898352532858	18.952886522081617	24.726982842128077	26.42923228325745
14	23.879996605040674	19.019137329611972	24.234012301817494	32.86685376352986
15	25.61584492050254	26.86636836851863	21.410198766451348	26.107587944527488
16	26.48454690780332	25.410220133175553	22.954370572593163	25.150862386427963
17	24.663011707699614	25.875447078967174	24.31944743529762	25.142093778035594
18	25.500397891333755	24.720993826985136	25.193974217202726	24.584634064478387
19	26.09331368372026	24.674795864041698	24.529142288777766	24.702748163460278
20	26.429612815862637	23.95886197036488	24.213379329066008	25.39814588470648
21	26.911515334314178	23.907325995121496	23.996759355869372	25.184399314694954
22	26.59280759629164	23.808520085322904	24.432168502212686	25.166503816172774
23	24.94106168234263	24.083834452243654	25.124592902991843	25.85051096242187
24	25.333528802950845	24.399834037877383	24.83280157881465	25.43383558035713
25	25.46876364520178	23.98605957961994	24.80632362271361	25.73885315246467
26	25.107671393920178	24.941219134784944	25.060499398863946	24.89061007243093
27	25.67406710123264	24.39446366782007	24.550358892200467	25.381110338746822
28	25.332354580294165	24.237570438478322	24.991416036562246	25.438658944665267
29	25.127092354888504	24.53493579885195	25.011494865626688	25.32647698063286
30	25.120359533664015	24.55211733524501	25.094476140105616	25.233046990985354
31	25.768367861209967	24.360434346647512	24.2144643564221	25.65673343572042
32	25.62960739125601	24.411444620467925	24.149805998118026	25.80914199015804
33	25.05885955039826	24.366012585930736	24.82415786038476	25.750970003286245
34	25.82967618209895	24.358258077209992	24.91636162640907	24.895704114281987
35	25.932766265781964	24.17586926840149	25.078552522255816	24.812811943560728
36	24.903762655701783	24.64335366101728	24.763054118775962	25.689829564504972
37	25.918380159109557	24.380945433272437	24.52688178454099	25.173792623077023
38	25.299952372368118	24.251969111481625	24.800493531690613	25.64758498445964
39	25.995319280419153	24.22295179939052	24.300566308849632	25.481162611340697
40	26.022837430434848	24.288741493306173	24.61396339333437	25.074457682924606
41	25.106286418135433	24.77618651135744	25.035650930220974	25.08187614028615
42	26.1169886656182	24.691773278693354	24.605422096643174	24.585815959045267
43	25.721633068787153	24.006969765415768	24.691635580035157	25.579761585761922
44	25.12450261210847	24.57928305996363	24.580650931467886	25.715563396460016
45	25.34989322140959	24.787047821867567	24.487317214658813	25.37574174206403
46	25.32305287952313	24.449186247648388	24.638893448315045	25.588867424513438
47	25.594553977108326	24.38343817020926	24.79606348783255	25.225944364849866
48	25.959558512770677	24.94573301379323	23.964104699070315	25.130603774365778
49	25.438938606816226	24.957351230851895	24.179116412901813	25.424593749430063
50	25.460366806382723	25.058193590971566	24.252514415561688	25.228925187084023
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	43.0
2	48.0
3	153.5
4	259.0
5	283.0
6	307.0
7	276.5
8	246.0
9	288.5
10	331.0
11	600.0
12	869.0
13	1660.0
14	2451.0
15	3167.0
16	3883.0
17	3839.5
18	3796.0
19	3769.0
20	3742.0
21	4439.0
22	5136.0
23	6163.5
24	7191.0
25	9399.0
26	11607.0
27	14857.5
28	18108.0
29	21716.5
30	25325.0
31	30347.0
32	35369.0
33	42073.5
34	48778.0
35	57676.5
36	66575.0
37	78636.5
38	90698.0
39	101427.5
40	112157.0
41	123066.5
42	133976.0
43	142398.0
44	150820.0
45	165521.5
46	180223.0
47	193925.0
48	207627.0
49	219304.5
50	230982.0
51	227023.0
52	223064.0
53	224184.5
54	225305.0
55	226750.0
56	228195.0
57	220593.0
58	212991.0
59	196045.0
60	179099.0
61	157506.0
62	135913.0
63	116540.5
64	97168.0
65	82328.0
66	67488.0
67	58229.5
68	48971.0
69	42577.5
70	36184.0
71	28226.5
72	20269.0
73	17573.5
74	14878.0
75	11189.5
76	7501.0
77	6386.0
78	5271.0
79	4522.0
80	3773.0
81	2907.0
82	2041.0
83	1715.0
84	1389.0
85	1080.5
86	772.0
87	519.0
88	266.0
89	169.0
90	72.0
91	55.5
92	39.0
93	37.0
94	35.0
95	28.5
96	22.0
97	23.5
98	25.0
99	19.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03282729266723858
2	0.004033267795654312
3	2.805751510020391E-4
4	0.0
5	3.5071893875254885E-5
6	2.1043136325152933E-4
7	0.0
8	0.0
9	0.0
10	3.5071893875254885E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.2086272650305865E-4
17	0.0
18	1.7535946937627443E-4
19	0.0
20	2.4550325712678416E-4
21	3.5071893875254885E-5
22	0.0
23	0.0
24	0.0011573724978834112
25	3.1564704487729395E-4
26	6.312940897545879E-4
27	4.2086272650305865E-4
28	0.0013678038611349406
29	4.559346203783135E-4
30	0.0012976600733844309
31	5.260784081288232E-4
32	8.767973468813722E-4
33	2.4550325712678416E-4
34	8.066535591308624E-4
35	3.1564704487729395E-4
36	7.014378775050977E-5
37	0.00150809143663596
38	0.0
39	5.260784081288232E-4
40	7.014378775050977E-5
41	9.820130285071367E-4
42	0.004839921354785174
43	0.003998195901779057
44	0.004945137036410939
45	0.0020692417386400383
46	0.0017535946937627443
47	0.0010872287101329015
48	0.0036825488569017626
49	0.0031564704487729396
50	9.469411346318819E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2851286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.78057032362725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28700486890187	34.061337492993246
2	15.619282446781222	14.925964467060538
3	5.447908238024731	7.809124880508267
4	2.5782910302439896	4.927688635414012
5	1.3253086600626216	3.166200181631715
6	0.8356367267888425	2.395631963360399
7	0.5492297781315987	1.836975842649293
8	0.3933490491932983	1.5035553525369842
9	0.2738504424505551	1.1776257291298668
>10	1.5155809340117	12.806529598494276
>50	0.09365273264614539	3.0827791597739793
>100	0.06954876929031596	6.67593418300705
>500	0.007595463102146735	2.6047471462736946
>1k	0.0037608603709268318	3.0259053671667417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3460	0.1213487528083819	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2897	0.1016032765566134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7535946937627443E-4
2	0.0	0.0	0.0	0.0	1.7535946937627443E-4
3	0.0	0.0	0.0	0.0	1.7535946937627443E-4
4	0.0	0.0	0.0	0.0	2.104313632515293E-4
5	0.0	3.5071893875254885E-5	0.0	0.0	2.455032571267842E-4
6	0.0	3.5071893875254885E-5	0.0	0.0	3.8579083262780373E-4
7	0.0	3.5071893875254885E-5	0.0	0.0	3.8579083262780373E-4
8	0.0	3.5071893875254885E-5	0.0	0.0	3.8579083262780373E-4
9	0.0	3.5071893875254885E-5	0.0	0.0	3.8579083262780373E-4
10	0.0	3.5071893875254885E-5	0.0	7.014378775050977E-5	3.8579083262780373E-4
11	0.0	3.5071893875254885E-5	0.0	7.014378775050977E-5	3.8579083262780373E-4
12	0.0	3.5071893875254885E-5	0.0	1.0521568162576465E-4	0.001227516285633921
13	0.0	3.5071893875254885E-5	0.0	1.0521568162576465E-4	0.0014379476488854503
14	0.0	3.5071893875254885E-5	0.0	1.0521568162576465E-4	0.0014379476488854503
15	0.0	3.5071893875254885E-5	0.0	1.4028757550101954E-4	0.0015782352243864698
16	0.0	3.5071893875254885E-5	0.0	1.7535946937627443E-4	0.0015782352243864698
17	0.0	3.5071893875254885E-5	0.0	2.104313632515293E-4	0.0015782352243864698
18	0.0	3.5071893875254885E-5	0.0	2.455032571267842E-4	0.0015782352243864698
19	0.0	3.5071893875254885E-5	0.0	4.208627265030586E-4	0.0015782352243864698
20	0.0	3.5071893875254885E-5	0.0	6.663659836298429E-4	0.0017535946937627443
21	0.0	3.5071893875254885E-5	0.0	7.715816652556075E-4	0.0017535946937627443
22	0.0	3.5071893875254885E-5	0.0	9.469411346318819E-4	0.001823738481513254
23	0.0	3.5071893875254885E-5	0.0	0.0011223006040081563	0.001823738481513254
24	0.0	3.5071893875254885E-5	0.0	0.0017185227998874895	0.001858810375388509
25	0.0	3.5071893875254885E-5	0.0	0.002455032571267842	0.001858810375388509
26	0.0	3.5071893875254885E-5	0.0	0.0032266142365234493	0.001858810375388509
27	0.0	3.5071893875254885E-5	0.0	0.0039280521140285475	0.001858810375388509
28	0.0	3.5071893875254885E-5	0.0	0.004874993248660429	0.0018938822692637638
29	0.0	7.014378775050977E-5	0.0	0.006137581428169605	0.0018938822692637638
30	0.0	7.014378775050977E-5	0.0	0.008697829681063212	0.0018938822692637638
31	0.0	7.014378775050977E-5	0.0	0.013467607248097875	0.0018938822692637638
32	0.0	7.014378775050977E-5	0.0	0.019184325949764422	0.0019990979508895286
33	0.0	7.014378775050977E-5	0.0	0.02339295321479501	0.002104313632515293
34	0.0	7.014378775050977E-5	0.0	0.02865373729608324	0.0022796731018915675
35	0.0	1.0521568162576465E-4	0.0	0.034510743573250804	0.0022796731018915675
36	0.0	1.0521568162576465E-4	0.0	0.04334886082981504	0.0022796731018915675
37	0.0	1.7535946937627443E-4	0.0	0.057903696788045814	0.0022796731018915675
38	0.0	1.7535946937627443E-4	0.0	0.07494863721141969	0.0023498168896420773
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3220	0.0	26.654535	1
GGTATCA	1585	0.0	23.742653	1
GTCCTAA	475	0.0	19.45889	1
CGAGCCG	850	0.0	17.599178	15
TCAACGC	4815	0.0	17.498665	4
ATCAACG	4865	0.0	17.40926	3
TAGGACG	495	0.0	17.332525	4
CGGATCG	245	0.0	17.061327	26
AACGCAG	4985	0.0	16.946049	6
ACCGTCG	710	0.0	16.731615	8
CGTCGTA	755	0.0	16.608498	10
CAACGCA	5085	0.0	16.52627	5
TATCAAC	5205	0.0	16.526226	2
ATACCGT	835	0.0	16.071108	6
GTAGGAC	955	0.0	15.894548	3
TACCGTC	780	0.0	15.794135	7
GTCGATA	85	0.0029814986	15.529233	36
TCTAGAT	770	0.0	15.428392	2
CGTCTTA	200	1.2805685E-9	15.3992815	15
CGCAATA	415	0.0	15.373315	36
>>END_MODULE