FastQCFastQC Report
Thu 2 Feb 2017
SRR4062005_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062005_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175978
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18950.16114247035233653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.015.3767491
CGAATTT400.005272754514.252171515
TTAGGGT551.9605161E-413.8173474
GTGTATA655.4642718E-513.1502521
CCTACAC1800.012.6642833
GTATCAA12950.012.2476171
GGATAAT701.090646E-412.21406656
GTCTTAA1251.4151738E-912.1566771
AATAGCG550.003068090212.09069255
CAGTACC802.8667384E-511.8742824
CGTTATT658.0364046E-411.689612
TCATATA658.0364046E-411.689612
TCTAGAC1406.8030204E-1011.5335443
ATAGCGT752.0733374E-411.39979656
TTATACC752.0741574E-411.3993114
GCCGTGC1303.2623575E-810.9613438
ACCGTCG1408.340066E-910.8569488
GTATTAG700.001494336410.8541761
GGGTATA700.001494336410.8541761
TAGACAG1158.8097113E-710.7389375
Produced by FastQC (version 0.11.5)