##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062005_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1175978
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31060189901512	32.0	32.0	32.0	32.0	32.0
2	30.98905676806879	32.0	32.0	32.0	32.0	32.0
3	30.997315425968853	32.0	32.0	32.0	32.0	32.0
4	31.007756097478016	32.0	32.0	32.0	32.0	32.0
5	30.975366886115218	32.0	32.0	32.0	32.0	32.0
6	34.596657420461945	36.0	36.0	36.0	32.0	36.0
7	34.54892098321567	36.0	36.0	36.0	32.0	36.0
8	34.52436950351112	36.0	36.0	36.0	32.0	36.0
9	34.61934917149811	36.0	36.0	36.0	32.0	36.0
10	34.412284923697555	36.0	36.0	36.0	32.0	36.0
11	34.5859701457	36.0	36.0	36.0	32.0	36.0
12	34.475331171161365	36.0	36.0	36.0	32.0	36.0
13	34.51689657459578	36.0	36.0	36.0	32.0	36.0
14	34.458939708055766	36.0	36.0	36.0	32.0	36.0
15	34.43222492257508	36.0	36.0	36.0	32.0	36.0
16	34.42465590342677	36.0	36.0	36.0	32.0	36.0
17	34.38285069958792	36.0	36.0	36.0	32.0	36.0
18	34.379837037767714	36.0	36.0	36.0	32.0	36.0
19	34.38705315915774	36.0	36.0	36.0	32.0	36.0
20	34.36788528356823	36.0	36.0	36.0	32.0	36.0
21	34.34487890079576	36.0	36.0	36.0	32.0	36.0
22	34.32751888215596	36.0	36.0	36.0	32.0	36.0
23	34.29687034961538	36.0	36.0	36.0	32.0	36.0
24	34.28291685728815	36.0	36.0	36.0	32.0	36.0
25	33.92959222026263	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	12.0
5	84.0
6	237.0
7	47.0
8	186.0
9	189.0
10	90.0
11	23.0
12	42.0
13	52.0
14	128.0
15	216.0
16	299.0
17	478.0
18	649.0
19	844.0
20	1315.0
21	1780.0
22	2577.0
23	3646.0
24	5349.0
25	7546.0
26	10753.0
27	13959.0
28	19159.0
29	26186.0
30	35120.0
31	49144.0
32	71094.0
33	104627.0
34	243670.0
35	576475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10563248534819	17.177821770054187	11.827913099380593	25.88863264521703
2	16.608473968050422	19.06540768619821	37.80504397191104	26.521074373840325
3	19.49940857284503	22.346337360933834	28.302616820681497	29.851637245539635
4	13.194898014722892	15.46805050958621	34.88607448531113	36.45097699037977
5	15.060577628936397	35.87064977088276	33.759555766889896	15.309216833290943
6	35.00531792694444	34.51683442953534	16.464727254162874	14.01312038935734
7	30.815159099034677	30.314878185811327	19.786420825033623	19.08354189012037
8	28.048752459031746	33.61972026412406	18.757421785552854	19.574105491291345
9	26.88729882391	15.090813898548936	18.446096939101267	39.575790338439795
10	16.265611110921903	27.04251398912203	31.610136877130724	25.081738022825345
11	36.362142314596255	21.80897445067765	22.04621085939868	19.782672375327415
12	24.308834823656028	23.609538292296246	28.67796939204711	23.403657492000622
13	29.30793149131381	20.0988839582191	24.8648795921356	25.72830495833149
14	23.530713796200523	19.317149582636308	25.243586305533206	31.908550315629963
15	24.970815853356367	26.890966665786813	22.330576556125948	25.807640924730872
16	25.419777985695664	26.29794254939303	23.527658991204735	24.75462047370657
17	23.928292598618537	26.101974286344316	25.130113549773487	24.839619565263664
18	24.21036412252796	25.95627601224837	25.503815058819924	24.32954480640375
19	25.29437399669409	25.38681007025482	25.36008361804665	23.958732315004436
20	25.43745345447582	24.964742430367895	25.27871991829266	24.31908419686363
21	25.786919375128736	24.775682655004946	25.068048460376986	24.369349509489336
22	25.583285877114353	24.60275101360525	25.1658413966344	24.648121712646
23	24.71145122883224	24.904214233313873	25.22191149412179	25.162423043732097
24	24.51788124788283	25.14771243610192	25.41760361148608	24.916802704529168
25	24.80939679340638	24.975360737526746	25.260988940843642	24.954253528223234
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	38.0
2	168.5
3	299.0
4	299.0
5	299.0
6	626.5
7	954.0
8	954.0
9	954.0
10	1209.0
11	1464.0
12	1464.0
13	1464.0
14	2206.0
15	2948.0
16	2948.0
17	2948.0
18	5231.0
19	7514.0
20	7514.0
21	7514.0
22	11286.5
23	15059.0
24	15059.0
25	15059.0
26	22673.0
27	30287.0
28	30287.0
29	30287.0
30	39745.5
31	49204.0
32	49204.0
33	49204.0
34	59822.5
35	70441.0
36	70441.0
37	70441.0
38	85011.5
39	99582.0
40	99582.0
41	99582.0
42	113805.5
43	128029.0
44	128029.0
45	128029.0
46	139192.5
47	150356.0
48	150356.0
49	150356.0
50	156232.5
51	162109.0
52	162109.0
53	162109.0
54	155306.5
55	148504.0
56	148504.0
57	148504.0
58	135146.0
59	121788.0
60	121788.0
61	121788.0
62	104673.5
63	87559.0
64	87559.0
65	87559.0
66	69773.5
67	51988.0
68	51988.0
69	51988.0
70	39137.0
71	26286.0
72	26286.0
73	26286.0
74	19319.5
75	12353.0
76	12353.0
77	12353.0
78	8872.5
79	5392.0
80	5392.0
81	5392.0
82	3823.5
83	2255.0
84	2255.0
85	2255.0
86	1565.5
87	876.0
88	876.0
89	876.0
90	553.5
91	231.0
92	231.0
93	231.0
94	149.0
95	67.0
96	67.0
97	67.0
98	231.0
99	395.0
100	395.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011904984617059165
2	0.0
3	7.65320439668089E-4
4	0.010374343737722985
5	0.03222849407046731
6	0.060205207920556336
7	0.10527407825656603
8	0.1043386866080828
9	0.11250210463120908
10	0.1270431929849028
11	0.13792775034907115
12	0.12857383386423896
13	0.1250023384791212
14	0.1142028167193604
15	0.13086979518324324
16	0.11641374243395711
17	0.12134580748959589
18	0.109780965290167
19	0.09455959210121279
20	0.08954249144116641
21	0.09183845276017069
22	0.10297811693756175
23	0.08163418023126283
24	0.09013774067201938
25	0.08707645891334702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1175978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.67483497003279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.0529960427587	26.054265305507922
2	16.656006944266036	14.215846952125464
3	7.915245653145464	10.133454059857563
4	4.493050630922614	7.669607767464968
5	2.70345261360524	5.768469706745371
6	1.7432066006070328	4.463463239974619
7	1.2438626752203388	3.715714407328801
8	0.8649360424237748	2.952880229605429
9	0.600612002911308	2.306791629473483
>10	2.6268586464044206	17.937104158082626
>50	0.06760003947793766	1.9541486610362129
>100	0.031372801990241984	2.456196188754182
>500	3.9965313349130544E-4	0.11614544563075598
>1k	3.9965313349130544E-4	0.2559122484125541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1895	0.16114247035233653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.700712088151309E-4
7	0.0	0.0	0.0	0.0	1.700712088151309E-4
8	0.0	0.0	0.0	0.0	1.700712088151309E-4
9	0.0	0.0	0.0	0.0	1.700712088151309E-4
10	0.0	0.0	0.0	0.0	1.700712088151309E-4
11	0.0	0.0	0.0	0.0	1.700712088151309E-4
12	0.0	0.0	0.0	0.0	1.700712088151309E-4
13	0.0	0.0	0.0	0.0	1.700712088151309E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	420	0.0	15.376749	1
CGAATTT	40	0.0052727545	14.2521715	15
TTAGGGT	55	1.9605161E-4	13.817347	4
GTGTATA	65	5.4642718E-5	13.150252	1
CCTACAC	180	0.0	12.664283	3
GTATCAA	1295	0.0	12.247617	1
GGATAAT	70	1.090646E-4	12.2140665	6
GTCTTAA	125	1.4151738E-9	12.156677	1
AATAGCG	55	0.0030680902	12.0906925	5
CAGTACC	80	2.8667384E-5	11.874282	4
CGTTATT	65	8.0364046E-4	11.68961	2
TCATATA	65	8.0364046E-4	11.68961	2
TCTAGAC	140	6.8030204E-10	11.533544	3
ATAGCGT	75	2.0733374E-4	11.3997965	6
TTATACC	75	2.0741574E-4	11.399311	4
GCCGTGC	130	3.2623575E-8	10.961343	8
ACCGTCG	140	8.340066E-9	10.856948	8
GTATTAG	70	0.0014943364	10.854176	1
GGGTATA	70	0.0014943364	10.854176	1
TAGACAG	115	8.8097113E-7	10.738937	5
>>END_MODULE