FastQCFastQC Report
Thu 2 Feb 2017
SRR4062005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175978
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT250.002353447735.1979267
GGTATCA6400.025.446821
GTATCAA11400.024.1316451
GTATATA1404.0618033E-918.8640521
CGGTATT600.007414506318.33225331
ACGGACC1601.1023076E-917.8739458
ATAATAC1509.596988E-917.599713
TTTATAC1509.596988E-917.599713
TTATACA2400.017.415644
TAGAGTG2300.017.2163775
TAATACT2300.017.2163774
ATAGCGT953.3477196E-416.209576
ATCAACG16450.015.647163
TATCAAC16900.015.49284652
TTAGAGT1857.570634E-915.4585494
CGACCAT2001.2769306E-915.39974610
TCAACGC16600.015.3725874
GTCCTAC1301.3641407E-515.236351
ATTATAC1301.3690929E-515.2305193
TATAGTA1452.282688E-615.1741012