##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1175978
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307939434241117	32.0	32.0	32.0	32.0	32.0
2	31.439401077231036	32.0	32.0	32.0	32.0	32.0
3	31.565464660053163	32.0	32.0	32.0	32.0	32.0
4	31.641327473813284	32.0	32.0	32.0	32.0	32.0
5	31.61159052295196	32.0	32.0	32.0	32.0	32.0
6	35.25946658866067	36.0	36.0	36.0	36.0	36.0
7	35.26553983152746	36.0	36.0	36.0	36.0	36.0
8	35.219198828549516	36.0	36.0	36.0	36.0	36.0
9	35.30208643358974	36.0	36.0	36.0	36.0	36.0
10	35.18599072431627	36.0	36.0	36.0	36.0	36.0
11	35.31161382270757	36.0	36.0	36.0	36.0	36.0
12	35.2405810312778	36.0	36.0	36.0	36.0	36.0
13	35.26920486607742	36.0	36.0	36.0	36.0	36.0
14	35.233239057193245	36.0	36.0	36.0	36.0	36.0
15	35.20455569747053	36.0	36.0	36.0	36.0	36.0
16	35.22668111138133	36.0	36.0	36.0	36.0	36.0
17	35.210465671976856	36.0	36.0	36.0	36.0	36.0
18	35.19999523800615	36.0	36.0	36.0	36.0	36.0
19	35.189199968026614	36.0	36.0	36.0	36.0	36.0
20	35.1950861325637	36.0	36.0	36.0	36.0	36.0
21	35.1852619691865	36.0	36.0	36.0	36.0	36.0
22	35.165914668471686	36.0	36.0	36.0	36.0	36.0
23	35.14318465141355	36.0	36.0	36.0	36.0	36.0
24	35.123106894856875	36.0	36.0	36.0	36.0	36.0
25	35.113974070943506	36.0	36.0	36.0	36.0	36.0
26	35.072111893249705	36.0	36.0	36.0	36.0	36.0
27	35.05420679638565	36.0	36.0	36.0	36.0	36.0
28	35.04195656721469	36.0	36.0	36.0	36.0	36.0
29	35.024360149594635	36.0	36.0	36.0	36.0	36.0
30	35.00761748944283	36.0	36.0	36.0	36.0	36.0
31	35.00570673941179	36.0	36.0	36.0	36.0	36.0
32	34.97167123874766	36.0	36.0	36.0	36.0	36.0
33	34.95452210840679	36.0	36.0	36.0	36.0	36.0
34	34.943435166304134	36.0	36.0	36.0	36.0	36.0
35	34.931686647199186	36.0	36.0	36.0	36.0	36.0
36	34.91122452971059	36.0	36.0	36.0	36.0	36.0
37	34.911968591249156	36.0	36.0	36.0	36.0	36.0
38	34.895087323062164	36.0	36.0	36.0	32.0	36.0
39	34.87088193826755	36.0	36.0	36.0	32.0	36.0
40	34.861998268675094	36.0	36.0	36.0	32.0	36.0
41	34.84360081566152	36.0	36.0	36.0	32.0	36.0
42	34.814461665099174	36.0	36.0	36.0	32.0	36.0
43	34.80179731253476	36.0	36.0	36.0	32.0	36.0
44	34.775920127757495	36.0	36.0	36.0	32.0	36.0
45	34.7599861562036	36.0	36.0	36.0	32.0	36.0
46	34.74584388483458	36.0	36.0	36.0	32.0	36.0
47	34.714738711098335	36.0	36.0	36.0	32.0	36.0
48	34.70298423950108	36.0	36.0	36.0	32.0	36.0
49	34.685945655445934	36.0	36.0	36.0	32.0	36.0
50	34.26337737610738	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	10.0
21	25.0
22	61.0
23	181.0
24	479.0
25	1153.0
26	2542.0
27	4794.0
28	8395.0
29	13823.0
30	20816.0
31	31566.0
32	48676.0
33	83852.0
34	193280.0
35	766324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.634264871132025	17.763781885243322	12.268976329191558	26.332976914433093
2	16.102296683513153	19.48154327743634	37.47062920471158	26.94553083433894
3	18.88149827460998	22.656607418921975	28.60890535950869	29.852988946959353
4	12.977963873473824	15.878103161793844	34.88271039084064	36.261222573891686
5	14.676222409111741	36.27970615071553	33.53330889974889	15.510762540423833
6	35.201003422691805	34.6446140436659	16.28002295967176	13.874359573970535
7	30.936548132703162	30.177520327761233	19.74169584805158	19.144235691484024
8	28.17093517055591	33.7166171475997	18.634192136247446	19.47825554559694
9	26.960878519836257	14.902829814843473	18.38954470236688	39.74674696295339
10	16.274893131413286	26.99780692989744	31.675704541840528	25.051595396848747
11	36.72755782846278	21.64700360040749	21.984254807487897	19.641183763641838
12	24.353091639469447	23.52824627671606	28.68837682337595	23.430285260438545
13	29.33889919709382	19.95751621203798	24.85522688349612	25.848357707372077
14	23.53071230924388	19.1687259455534	25.23839731695661	32.062164428246106
15	25.05029856000707	26.93621819455806	22.2181027196087	25.79538052582616
16	25.4454821666824	26.297882689483515	23.511254084915215	24.74538105891887
17	24.150281722957402	26.22897707270034	24.999957482197797	24.620783722144463
18	24.52800987773581	25.881606628695437	25.387974945109516	24.20240854845924
19	25.454642858965048	25.42003336797117	25.179212536288944	23.946111236774836
20	25.74778248214038	24.80176057865077	25.089266201634896	24.36119073757395
21	25.99191990999824	24.68492155884001	24.881353972058978	24.441804559102774
22	25.678626640974578	24.6099842003847	25.018835386376274	24.692553772264446
23	24.940730082926862	24.70135444941053	25.076192031129885	25.281723436532715
24	24.754520337799743	25.11465071605144	25.200537772021736	24.930291174127078
25	25.11792322468892	24.741810426564463	25.126937006421045	25.013329342325573
26	24.78290373272924	25.1786601087097	25.308169554480703	24.730266604080356
27	25.0276154299461	25.235613402688667	25.02591471062686	24.710856456738377
28	25.216439632879606	24.843808861333223	25.20674545032454	24.733006055462628
29	24.723250684113765	25.353621763746474	25.138906132278432	24.784221419861325
30	25.014179795555837	25.380515982989348	24.910860745478836	24.694443475975984
31	25.109016201066016	25.082229849010012	24.894725384618003	24.914028565305976
32	24.898870977479927	25.428730081387922	24.784412567295337	24.887986373836814
33	24.8533772344187	25.172175986435107	25.060439107227438	24.914007671918753
34	25.118583360403278	25.054295787463243	25.15319320456544	24.673927647568043
35	25.121686156860346	24.963264556419517	25.315907807642336	24.599141479077804
36	24.8326490766787	25.251706673288137	25.180616545291894	24.73502770474127
37	25.400203917980413	24.72144106574782	25.139906612708053	24.738448403563716
38	25.06586007561366	25.05676126594205	25.040179323082572	24.837199335361714
39	25.298881177990236	25.15278889154391	24.686790213007313	24.861539717458538
40	25.202172149478987	25.159654347275207	24.909564634712556	24.728608868533257
41	24.954930593113396	25.044559187186	25.217608702307214	24.78290151739339
42	25.174501073198606	25.10349308288631	25.074324531177183	24.647681312737898
43	25.00110550610069	24.76784371885449	25.185980141708875	25.045070633335943
44	24.81246741937651	25.076939168946428	25.07124154604572	25.039351865631343
45	24.895021046813216	25.221140354606913	25.06586164377737	24.8179769548025
46	25.208575512796855	25.06265131344535	24.737978473629475	24.990794700128323
47	24.97531822692605	25.14292468593985	25.228471158678502	24.6532859284556
48	25.05678489092454	25.315303900150433	24.711355872675984	24.91655533624904
49	24.98420411262829	25.03854366595546	24.931735404642588	25.04551681677366
50	24.93390489618243	25.337150933239904	24.831009977405635	24.89793419317203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	6.5
2	8.0
3	34.5
4	61.0
5	72.0
6	83.0
7	86.5
8	90.0
9	106.5
10	123.0
11	199.0
12	275.0
13	504.0
14	733.0
15	1015.5
16	1298.0
17	1320.5
18	1343.0
19	1475.0
20	1607.0
21	2001.0
22	2395.0
23	3177.0
24	3959.0
25	5222.5
26	6486.0
27	8234.0
28	9982.0
29	12202.0
30	14422.0
31	17119.0
32	19816.0
33	23102.0
34	26388.0
35	29973.0
36	33558.0
37	38102.5
38	42647.0
39	47344.5
40	52042.0
41	56966.0
42	61890.0
43	64934.5
44	67979.0
45	72870.5
46	77762.0
47	81121.0
48	84480.0
49	87159.0
50	89838.0
51	91278.5
52	92719.0
53	93497.5
54	94276.0
55	91156.5
56	88037.0
57	84015.0
58	79993.0
59	74025.5
60	68058.0
61	59406.0
62	50754.0
63	43178.0
64	35602.0
65	29589.5
66	23577.0
67	19910.0
68	16243.0
69	13246.0
70	10249.0
71	8177.0
72	6105.0
73	5410.0
74	4715.0
75	3629.5
76	2544.0
77	2099.5
78	1655.0
79	1377.5
80	1100.0
81	841.0
82	582.0
83	433.0
84	284.0
85	203.5
86	123.0
87	83.0
88	43.0
89	27.5
90	12.0
91	12.0
92	12.0
93	9.5
94	7.0
95	8.5
96	10.0
97	8.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033589063740988356
2	0.005527314286491754
3	0.0010204272528907853
4	0.0
5	8.503560440756545E-5
6	2.5510681322269633E-4
7	0.0
8	0.0
9	0.0
10	8.503560440756545E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.2517802203782725E-4
17	0.0
18	0.0
19	0.0
20	8.503560440756545E-5
21	8.503560440756545E-5
22	0.0
23	1.700712088151309E-4
24	0.0012755340661134817
25	8.503560440756545E-5
26	1.700712088151309E-4
27	4.2517802203782725E-4
28	0.0012755340661134817
29	3.401424176302618E-4
30	7.65320439668089E-4
31	5.102136264453927E-4
32	4.2517802203782725E-4
33	8.503560440756545E-5
34	0.0010204272528907853
35	1.700712088151309E-4
36	5.102136264453927E-4
37	0.0012755340661134817
38	0.0
39	7.65320439668089E-4
40	0.0
41	0.0011904984617059165
42	0.004591922638008534
43	0.00391163780274801
44	0.004166744615970708
45	0.002380996923411833
46	0.002125890110189136
47	7.65320439668089E-4
48	0.004166744615970708
49	0.003316388571895052
50	0.0017857476925588745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1175978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.77969405076401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.02020694012923	25.67646089762271
2	17.00470277823233	14.549119645539113
3	8.18262646025052	10.5015076950361
4	4.6283236261995935	7.919930747869648
5	2.8049836392164273	5.999817095153868
6	1.8279850958336494	4.692038587747202
7	1.2374161544688276	3.7055399151154558
8	0.901601261963046	3.0856180914049474
9	0.6665892305150659	2.5664835005071844
>10	2.6414815776351177	17.850168090400942
>50	0.06272877921538204	1.806559002175128
>100	0.021354456340840277	1.646756731427741
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5510681322269633E-4
2	0.0	0.0	0.0	0.0	2.5510681322269633E-4
3	0.0	0.0	0.0	0.0	2.5510681322269633E-4
4	0.0	0.0	0.0	0.0	2.5510681322269633E-4
5	0.0	0.0	0.0	0.0	2.5510681322269633E-4
6	0.0	0.0	0.0	0.0	2.5510681322269633E-4
7	0.0	0.0	0.0	0.0	2.5510681322269633E-4
8	0.0	0.0	0.0	0.0	2.5510681322269633E-4
9	0.0	0.0	0.0	0.0	2.5510681322269633E-4
10	0.0	0.0	0.0	0.0	2.5510681322269633E-4
11	0.0	0.0	0.0	0.0	2.5510681322269633E-4
12	0.0	0.0	0.0	0.0	6.802848352605236E-4
13	0.0	0.0	0.0	0.0	6.802848352605236E-4
14	0.0	0.0	0.0	0.0	6.802848352605236E-4
15	0.0	0.0	0.0	0.0	6.802848352605236E-4
16	0.0	0.0	0.0	0.0	6.802848352605236E-4
17	0.0	0.0	0.0	0.0	6.802848352605236E-4
18	0.0	0.0	0.0	0.0	6.802848352605236E-4
19	0.0	0.0	0.0	0.0	6.802848352605236E-4
20	0.0	0.0	0.0	0.0	6.802848352605236E-4
21	0.0	0.0	0.0	0.0	6.802848352605236E-4
22	0.0	0.0	0.0	0.0	6.802848352605236E-4
23	0.0	0.0	0.0	0.0	6.802848352605236E-4
24	0.0	0.0	0.0	0.0	6.802848352605236E-4
25	0.0	0.0	0.0	1.700712088151309E-4	6.802848352605236E-4
26	0.0	0.0	0.0	4.2517802203782725E-4	6.802848352605236E-4
27	0.0	0.0	0.0	4.2517802203782725E-4	8.503560440756545E-4
28	0.0	0.0	0.0	5.952492308529581E-4	9.3539164848322E-4
29	0.0	0.0	0.0	7.65320439668089E-4	9.3539164848322E-4
30	0.0	0.0	0.0	9.3539164848322E-4	9.3539164848322E-4
31	0.0	0.0	0.0	0.0027211393410420943	0.0010204272528907853
32	0.0	0.0	0.0	0.00391163780274801	0.0010204272528907853
33	0.0	0.0	0.0	0.005272207473269058	0.0010204272528907853
34	0.0	0.0	0.0	0.00748313318786576	0.0010204272528907853
35	0.0	0.0	0.0	0.009949165715685158	0.0010204272528907853
36	0.0	0.0	0.0	0.013860803518433168	0.0010204272528907853
37	0.0	0.0	0.0	0.019473153409332488	0.0011054628572983508
38	0.0	0.0	0.0	0.030357710773500864	0.0011904984617059162
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGAT	25	0.0023534477	35.197926	7
GGTATCA	640	0.0	25.44682	1
GTATCAA	1140	0.0	24.131645	1
GTATATA	140	4.0618033E-9	18.864052	1
CGGTATT	60	0.0074145063	18.332253	31
ACGGACC	160	1.1023076E-9	17.873945	8
ATAATAC	150	9.596988E-9	17.59971	3
TTTATAC	150	9.596988E-9	17.59971	3
TTATACA	240	0.0	17.41564	4
TAGAGTG	230	0.0	17.216377	5
TAATACT	230	0.0	17.216377	4
ATAGCGT	95	3.3477196E-4	16.20957	6
ATCAACG	1645	0.0	15.64716	3
TATCAAC	1690	0.0	15.4928465	2
TTAGAGT	185	7.570634E-9	15.458549	4
CGACCAT	200	1.2769306E-9	15.399746	10
TCAACGC	1660	0.0	15.372587	4
GTCCTAC	130	1.3641407E-5	15.23635	1
ATTATAC	130	1.3690929E-5	15.230519	3
TATAGTA	145	2.282688E-6	15.174101	2
>>END_MODULE