FastQCFastQC Report
Thu 2 Feb 2017
SRR4062004_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062004_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257874
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG9740.3777038398597765No Hit
TTGTAGAACAGTGTATATCAATGAG8860.3435786469361006No Hit
GTATCAACGCAGAGTACTTTTTTTT8850.343190860652877No Hit
GTGTATATCAATGAGTTACAATGAA8200.31798475224334366No Hit
GTGTATATCAATGAGTTACAATGAG7050.27338932967263085No Hit
GTCCTACAGTGGACATTTCTAAATT6360.2466320761302031No Hit
GTCCTAAAGTGTGTATTTCTCATTT6280.2435297858644144No Hit
TATCAACGCAGAGTACTTTTTTTTT5830.22607940311935287No Hit
CTTTAGGACGTGAAATATGGCGAGG5790.2245282579864585No Hit
GATATACACTGTTCTACAATGCCGG5780.22414047170323492No Hit
GTTCTACAGTGTGGTTTTTATCATT5730.22220154028711694No Hit
CTGTAGGACGTGGAATATGGCAAGA5330.20669008895817337No Hit
GTCCTACAGTGTGCATTTCTCATTT5250.20358779869238466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5170.20048550842659596No Hit
GTTCTAAGTTGGTTGTTAAGCGTAC5040.19544428674468928No Hit
ATCCTAAGCCATAGGGAAACTCCGT4710.1826473393983108No Hit
TATCTACATTGTTCTATCAACTAGA4320.1675236743525908No Hit
ATGTAGATAAGGGAAGTCGGCAAAA4170.16170688010423695No Hit
CTGTAGAACATATTAGATGAGTGAG4170.16170688010423695No Hit
GTTCTACAAATCCCGTTTCCAACGA4140.16054352125456617No Hit
CTGTAGGACCTGGAATATGGCGAGA4060.15744123098877746No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3970.15395115443976518No Hit
GTCCCACACCTTTGTGGGATCCGGT3850.14929771904108208No Hit
GTATCAACGCAGAGTACATGGGCAG3690.14309313850950464No Hit
GGTATCAACGCAGAGTACTTTTTTT3480.13494962656180925No Hit
CCTTTGTGGGATCCGGTGCGCCCCC3410.1322351225792441No Hit
CTTGTGCGTCGCCCGGCGAAAGGGA3360.13029619116312618No Hit
CCCCTTGTCCGTACCAGTTCTAAGT3340.12952061859667902No Hit
CCATAGGGTCTTCTCGTCTTATTAT3260.12641832833089028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3230.1252549694812195No Hit
GTCCGTACCAGTTCTAAGTTGGTTG3160.12254046549865438No Hit
CTCCACGGCAACGTAACTGAAAGCG3060.11866260266641848No Hit
GTAGAACAGTGTATATCAATGAGTT3020.11711145753352413No Hit
ATGTTCTACAGTGTGGTTTTTATCA3010.11672367125030053No Hit
GTAGAACATATTAGATGAGTGAGTT2970.11517252611740617No Hit
GTCGTACTCATAACCGCAGCAGGTC2740.10625344160326361No Hit
GTTTTAATTAGACAGTCAGATTCCC2740.10625344160326361No Hit
CCCTTAGAGCCAATCCTTTTCCCGA2720.10547786903681643No Hit
GTACATGGGCAGAAATCACATTGCG2620.10160000620458053No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2610.10121221992135693No Hit
TTGATATACACTGTTCTACAAATCC2580.10004886107168617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAG250.006015879619.0015533
GGGGTCC401.277519E-519.0015536
AACCTAT250.006015879619.0015536
TGGCTAG307.704668E-418.99786618
CTCTACT250.00602263318.9978641
ATAGGGG453.517953E-516.890273
GGGTCCT453.517953E-516.890277
ACTGTGC402.7481955E-416.626368
GCTGCTT350.00216241216.28704612
GTATAGG601.4569541E-615.8315541
TATAGGG551.120165E-515.5467252
TCGCCCG508.662994E-515.2012429
GTCGCCC508.662994E-515.2012428
GAATCCA508.662994E-515.2012429
TCCTAGG508.662994E-515.20124210
AGGGGTC508.662994E-515.2012425
TTGTAGA1850.014.8902181
TAACCTA456.731091E-414.7789865
CTGCTTT456.741013E-414.77611713
TGTGCCT400.00526043914.25116510
Produced by FastQC (version 0.11.5)