##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062004_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257874
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25538441254256	32.0	32.0	32.0	32.0	32.0
2	30.940358469640213	32.0	32.0	32.0	32.0	32.0
3	30.976663021475606	32.0	32.0	32.0	32.0	32.0
4	30.96619279182857	32.0	32.0	32.0	32.0	32.0
5	30.965393952085126	32.0	32.0	32.0	32.0	32.0
6	34.60607118205015	36.0	36.0	36.0	32.0	36.0
7	34.509946718164684	36.0	36.0	36.0	32.0	36.0
8	34.46262128016008	36.0	36.0	36.0	32.0	36.0
9	34.54134189565447	36.0	36.0	36.0	32.0	36.0
10	34.32594212677509	36.0	36.0	36.0	32.0	36.0
11	34.54352901029185	36.0	36.0	36.0	32.0	36.0
12	34.419305552324005	36.0	36.0	36.0	32.0	36.0
13	34.46346665425751	36.0	36.0	36.0	32.0	36.0
14	34.42017419359843	36.0	36.0	36.0	32.0	36.0
15	34.38651434421462	36.0	36.0	36.0	32.0	36.0
16	34.39549547453407	36.0	36.0	36.0	32.0	36.0
17	34.35354475441495	36.0	36.0	36.0	32.0	36.0
18	34.3585316860172	36.0	36.0	36.0	32.0	36.0
19	34.28983534594414	36.0	36.0	36.0	32.0	36.0
20	34.238705724501116	36.0	36.0	36.0	32.0	36.0
21	34.19351311105424	36.0	36.0	36.0	32.0	36.0
22	34.17826923226072	36.0	36.0	36.0	32.0	36.0
23	34.1432366194343	36.0	36.0	36.0	32.0	36.0
24	34.11250455648883	36.0	36.0	36.0	32.0	36.0
25	33.80073989622839	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	20.0
6	58.0
7	17.0
8	47.0
9	38.0
10	24.0
11	7.0
12	10.0
13	6.0
14	61.0
15	67.0
16	119.0
17	174.0
18	198.0
19	286.0
20	449.0
21	577.0
22	766.0
23	1025.0
24	1356.0
25	1828.0
26	2521.0
27	3030.0
28	4216.0
29	5593.0
30	7656.0
31	10524.0
32	15044.0
33	22758.0
34	53145.0
35	126249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79360222747032	19.62880665138771	12.223165846489135	24.35442527465283
2	14.015759634550207	20.721747830335747	43.06289117941321	22.199601355700846
3	20.128434817003654	24.796606094449228	30.29968123967519	24.77527784887193
4	11.471830412373134	17.02314145100427	38.453901314335134	33.051126822287465
5	11.58886190657222	39.47405171811841	36.21082930538207	12.726257069927302
6	29.702136576355638	40.25347106347642	17.34484016422069	12.699552195947255
7	25.869057139973133	32.85024342141021	21.105839784453643	20.17485965416301
8	24.728039320738894	38.50466273769868	19.572239434104375	17.19505850745805
9	25.926112869095917	14.218322300091243	20.34478072644678	39.51078410436606
10	14.906779233185894	29.153770577110528	33.21113600795318	22.7283141817504
11	34.80226339765971	22.785439377992848	24.45114160216553	17.961155622181916
12	22.07577869855114	25.16416646926563	33.08182499524296	19.678229836940268
13	29.070024813317648	22.108704853547064	26.81003250195128	22.011237831184012
14	20.513835987156043	22.95954619049283	26.86126738962466	29.665350432726466
15	24.23131586918196	31.42848266917264	23.61038840003417	20.729813061611228
16	22.52213076564684	27.779934772480196	28.661671066935863	21.0362633949371
17	19.08676153681881	28.30301500708614	29.34128016463142	23.26894329146363
18	19.64755759509888	29.11784574530038	30.784162997818104	20.450433661782633
19	24.166951336915	25.659141020465203	26.06246700413031	24.111440638489487
20	22.826221973790446	29.1037823737656	27.791363890441588	20.278631762002362
21	23.42028647956213	25.859244594542137	26.599899072240984	24.12056985365475
22	22.25008734811134	28.601265577079854	27.165262626654762	21.98338444815404
23	23.858356654621108	27.864830980503275	26.88126132909992	21.395551035775696
24	23.642186165670367	28.187252542504464	27.07903113112336	21.09153016070181
25	23.207527395106727	27.79531012852313	28.1046817199042	20.892480756465943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	569.0
1	569.0
2	374.5
3	180.0
4	180.0
5	180.0
6	396.0
7	612.0
8	612.0
9	612.0
10	617.0
11	622.0
12	622.0
13	622.0
14	683.0
15	744.0
16	744.0
17	744.0
18	1563.5
19	2383.0
20	2383.0
21	2383.0
22	4167.0
23	5951.0
24	5951.0
25	5951.0
26	10423.5
27	14896.0
28	14896.0
29	14896.0
30	20370.0
31	25844.0
32	25844.0
33	25844.0
34	26203.5
35	26563.0
36	26563.0
37	26563.0
38	26874.5
39	27186.0
40	27186.0
41	27186.0
42	26782.5
43	26379.0
44	26379.0
45	26379.0
46	30069.0
47	33759.0
48	33759.0
49	33759.0
50	31466.0
51	29173.0
52	29173.0
53	29173.0
54	26124.0
55	23075.0
56	23075.0
57	23075.0
58	19112.5
59	15150.0
60	15150.0
61	15150.0
62	12504.5
63	9859.0
64	9859.0
65	9859.0
66	8470.5
67	7082.0
68	7082.0
69	7082.0
70	5701.0
71	4320.0
72	4320.0
73	4320.0
74	3387.0
75	2454.0
76	2454.0
77	2454.0
78	1576.5
79	699.0
80	699.0
81	699.0
82	438.5
83	178.0
84	178.0
85	178.0
86	131.5
87	85.0
88	85.0
89	85.0
90	52.5
91	20.0
92	20.0
93	20.0
94	18.5
95	17.0
96	17.0
97	17.0
98	45.5
99	74.0
100	74.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011633588496707695
2	0.0
3	0.0
4	0.009694657080589746
5	0.035676338056570264
6	0.06669924071445744
7	0.1147847398341826
8	0.11633588496707695
9	0.12370382434832515
10	0.14231756594305747
11	0.14929771904108208
12	0.13999084824371594
13	0.13611298541148004
14	0.12447939691477233
15	0.13805191682759796
16	0.1217648929322072
17	0.12758168718056104
18	0.11633588496707695
19	0.10315115133747489
20	0.10004886107168617
21	0.10237557877102771
22	0.1101313044354995
23	0.09345649425688514
24	0.09849771593879182
25	0.09888550222201539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	257874.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.71376718862701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88709975939294	26.35705809814095
2	14.84154893371995	11.19461442409859
3	5.68511320871121	6.432211079829685
4	2.578814239003023	3.8902719932990526
5	1.5094494827976228	2.8463513188611493
6	0.9254118082546734	2.094045929407385
7	0.6220823822156415	1.642274909451903
8	0.4390564912497172	1.3246779434917828
9	0.42054825508462373	1.4274413085460342
>10	2.6024636518806425	19.42499049923606
>50	0.299216484669011	7.890675291033605
>100	0.17377177288337753	11.626220557326446
>500	0.01542353013757789	3.849166647277352
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	974	0.3777038398597765	No Hit
TTGTAGAACAGTGTATATCAATGAG	886	0.3435786469361006	No Hit
GTATCAACGCAGAGTACTTTTTTTT	885	0.343190860652877	No Hit
GTGTATATCAATGAGTTACAATGAA	820	0.31798475224334366	No Hit
GTGTATATCAATGAGTTACAATGAG	705	0.27338932967263085	No Hit
GTCCTACAGTGGACATTTCTAAATT	636	0.2466320761302031	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	628	0.2435297858644144	No Hit
TATCAACGCAGAGTACTTTTTTTTT	583	0.22607940311935287	No Hit
CTTTAGGACGTGAAATATGGCGAGG	579	0.2245282579864585	No Hit
GATATACACTGTTCTACAATGCCGG	578	0.22414047170323492	No Hit
GTTCTACAGTGTGGTTTTTATCATT	573	0.22220154028711694	No Hit
CTGTAGGACGTGGAATATGGCAAGA	533	0.20669008895817337	No Hit
GTCCTACAGTGTGCATTTCTCATTT	525	0.20358779869238466	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	517	0.20048550842659596	No Hit
GTTCTAAGTTGGTTGTTAAGCGTAC	504	0.19544428674468928	No Hit
ATCCTAAGCCATAGGGAAACTCCGT	471	0.1826473393983108	No Hit
TATCTACATTGTTCTATCAACTAGA	432	0.1675236743525908	No Hit
ATGTAGATAAGGGAAGTCGGCAAAA	417	0.16170688010423695	No Hit
CTGTAGAACATATTAGATGAGTGAG	417	0.16170688010423695	No Hit
GTTCTACAAATCCCGTTTCCAACGA	414	0.16054352125456617	No Hit
CTGTAGGACCTGGAATATGGCGAGA	406	0.15744123098877746	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	397	0.15395115443976518	No Hit
GTCCCACACCTTTGTGGGATCCGGT	385	0.14929771904108208	No Hit
GTATCAACGCAGAGTACATGGGCAG	369	0.14309313850950464	No Hit
GGTATCAACGCAGAGTACTTTTTTT	348	0.13494962656180925	No Hit
CCTTTGTGGGATCCGGTGCGCCCCC	341	0.1322351225792441	No Hit
CTTGTGCGTCGCCCGGCGAAAGGGA	336	0.13029619116312618	No Hit
CCCCTTGTCCGTACCAGTTCTAAGT	334	0.12952061859667902	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	326	0.12641832833089028	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	323	0.1252549694812195	No Hit
GTCCGTACCAGTTCTAAGTTGGTTG	316	0.12254046549865438	No Hit
CTCCACGGCAACGTAACTGAAAGCG	306	0.11866260266641848	No Hit
GTAGAACAGTGTATATCAATGAGTT	302	0.11711145753352413	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	301	0.11672367125030053	No Hit
GTAGAACATATTAGATGAGTGAGTT	297	0.11517252611740617	No Hit
GTCGTACTCATAACCGCAGCAGGTC	274	0.10625344160326361	No Hit
GTTTTAATTAGACAGTCAGATTCCC	274	0.10625344160326361	No Hit
CCCTTAGAGCCAATCCTTTTCCCGA	272	0.10547786903681643	No Hit
GTACATGGGCAGAAATCACATTGCG	262	0.10160000620458053	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	261	0.10121221992135693	No Hit
TTGATATACACTGTTCTACAAATCC	258	0.10004886107168617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGAG	25	0.0060158796	19.001553	3
GGGGTCC	40	1.277519E-5	19.001553	6
AACCTAT	25	0.0060158796	19.001553	6
TGGCTAG	30	7.704668E-4	18.997866	18
CTCTACT	25	0.006022633	18.997864	1
ATAGGGG	45	3.517953E-5	16.89027	3
GGGTCCT	45	3.517953E-5	16.89027	7
ACTGTGC	40	2.7481955E-4	16.62636	8
GCTGCTT	35	0.002162412	16.287046	12
GTATAGG	60	1.4569541E-6	15.831554	1
TATAGGG	55	1.120165E-5	15.546725	2
TCGCCCG	50	8.662994E-5	15.201242	9
GTCGCCC	50	8.662994E-5	15.201242	8
GAATCCA	50	8.662994E-5	15.201242	9
TCCTAGG	50	8.662994E-5	15.201242	10
AGGGGTC	50	8.662994E-5	15.201242	5
TTGTAGA	185	0.0	14.890218	1
TAACCTA	45	6.731091E-4	14.778986	5
CTGCTTT	45	6.741013E-4	14.776117	13
TGTGCCT	40	0.005260439	14.251165	10
>>END_MODULE