FastQCFastQC Report
Thu 2 Feb 2017
SRR4062004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257874
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG5630.21832367745488107No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC4530.1756671863002862No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC4510.17489161373383902No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA4480.17372825488416824No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA4310.1671358880693672No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGC3990.15472672700621234No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGG3930.15240000930687078No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTG3850.14929771904108208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.144644283642399No Hit
GTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATT3730.144644283642399No Hit
CTTGTGCGTCGCCCGGCGAAAGGGAAACCGGTTAACATTCCGGTACCTGG3260.12641832833089028No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT3090.11982596151608926No Hit
CCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAA3050.11827481638319488No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2980.11556031240062976No Hit
CTCCACGGCAACGTAACTGAAAGCGAAGACGACGGCGGGGGCCCTGGGAA2970.11517252611740617No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2780.10780458673615796No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTT2730.10586565532004003No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAG2620.10160000620458053No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGG2610.10121221992135693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1750.033.9394154
ATTGCTT300.005739585529.330368
GACCTAG300.005739585529.330367
ATCTGAC300.005739585529.3303627
GGAATCT454.060092E-529.33035924
TGTAGGA4150.029.1536712
GTGATCG703.571222E-828.2828469
AGGACCT3000.027.8638445
GTAAGAC407.0211943E-427.4972133
TACAACG407.0211943E-427.49721328
GGCTAGA407.0211943E-427.49721319
CAAGTAG655.8860496E-727.084681
GGGGTCC655.9060403E-727.0741776
AATCTGA655.9060403E-727.07417726
CTGTAGG4000.026.9577221
GGACCTG2700.026.8861646
GTAGGAC4850.026.7601743
ACTATAC609.721847E-625.6640663
AGGGGTC609.721847E-625.6640665
AGCTCCA609.721847E-625.66406614
Produced by FastQC (version 0.11.5)