FastQCFastQC Report
Thu 2 Feb 2017
SRR4062003_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062003_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556555
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27030.1736527138456399No Hit
TATCAACGCAGAGTACTTTTTTTTT17440.11204229853747538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.014.7713511
GTACGCT400.005281950314.2488423
GTATCAA20350.013.8121731
ACCGTTA1001.00499165E-813.3019148
CGCGTAA801.9961735E-613.0643810
TCGCGTA905.391321E-712.6693069
CGATAAC751.4763971E-512.6684910
ACGAACG701.0907458E-412.21447215
TTAGGAC2100.012.2132933
TACGGCC1103.8262442E-812.08798319
GTATTAG1103.8366124E-812.085651
TAGGACG1353.7107384E-1011.9631454
CCGTTAT1209.980795E-911.8774739
GTCCTAT1850.011.8056751
GCGTAAC907.4431737E-611.6135311
TCGAACT752.0784838E-411.39724119
CGGTTTT600.005873588411.08385814
GCCTAAT600.00589471811.0785131
GGCCTAA951.3666588E-510.9952151
ATAACGA700.001489613110.858705512
Produced by FastQC (version 0.11.5)