##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062003_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1556555
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293766683477294	32.0	32.0	32.0	32.0	32.0
2	30.87320011178532	32.0	32.0	32.0	32.0	32.0
3	30.90156146104699	32.0	32.0	32.0	32.0	32.0
4	30.901132950650634	32.0	32.0	32.0	32.0	32.0
5	30.845373276241443	32.0	32.0	32.0	32.0	32.0
6	34.49664676159853	36.0	36.0	36.0	32.0	36.0
7	34.44846407611681	36.0	36.0	36.0	32.0	36.0
8	34.39409786355124	36.0	36.0	36.0	32.0	36.0
9	34.52188583121059	36.0	36.0	36.0	32.0	36.0
10	34.2433482915798	36.0	36.0	36.0	32.0	36.0
11	34.4990334424418	36.0	36.0	36.0	32.0	36.0
12	34.34390561207282	36.0	36.0	36.0	32.0	36.0
13	34.42231851749537	36.0	36.0	36.0	32.0	36.0
14	34.33145375524797	36.0	36.0	36.0	32.0	36.0
15	34.29253447517113	36.0	36.0	36.0	32.0	36.0
16	34.291392209077095	36.0	36.0	36.0	32.0	36.0
17	34.23132173292945	36.0	36.0	36.0	32.0	36.0
18	34.229923131530846	36.0	36.0	36.0	32.0	36.0
19	34.238896152079434	36.0	36.0	36.0	32.0	36.0
20	34.218930908319976	36.0	36.0	36.0	32.0	36.0
21	34.20728339184931	36.0	36.0	36.0	32.0	36.0
22	34.175427787646434	36.0	36.0	36.0	32.0	36.0
23	34.14573336631214	36.0	36.0	36.0	32.0	36.0
24	34.12204836963679	36.0	36.0	36.0	32.0	36.0
25	33.781708966274884	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	19.0
5	106.0
6	309.0
7	74.0
8	269.0
9	207.0
10	113.0
11	28.0
12	77.0
13	77.0
14	233.0
15	366.0
16	578.0
17	752.0
18	991.0
19	1377.0
20	1997.0
21	2874.0
22	4027.0
23	6065.0
24	8444.0
25	12103.0
26	16741.0
27	21166.0
28	28238.0
29	37731.0
30	49873.0
31	67861.0
32	97605.0
33	138988.0
34	313677.0
35	743589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.411320129235605	17.169010437914423	11.664611891654358	25.755057541195615
2	17.067467068238003	18.703647549198486	37.52807487318124	26.70081050938228
3	19.870790425693894	21.934982759850204	27.666730269231582	30.527496545224313
4	13.332690831172918	14.858569211938974	34.098681906817916	37.710058050070195
5	15.418211169777951	35.846332008616656	32.87246731480101	15.862989506804382
6	35.88015968423151	33.77295782253451	16.05308664990968	14.293795843324311
7	31.713337376463198	29.68073208519385	19.11948091872087	19.486449619622086
8	28.55007627657139	33.25478432895911	18.184373207243315	20.010766187226185
9	26.79977770516604	14.955824047464064	18.438443112848073	39.80595513452182
10	16.830901651999383	26.652771344758374	30.229916113426995	26.286410889815244
11	37.189847634745426	21.452793663231805	21.33679714505741	20.020561556965355
12	24.7863429307735	23.392497926955496	28.002408527112333	23.818750615158667
13	29.558802528333107	19.539967398757963	24.78517788519062	26.116052187718314
14	23.50141760401766	19.513602309330974	24.561790554795156	32.42318953185621
15	25.201805895794305	27.103811989213	21.833570928628777	25.860811186363918
16	25.98259833702005	26.007876867186084	22.957472249898533	25.052052545895325
17	24.34429103025508	26.113539365051796	24.670931388157197	24.871238216535925
18	24.81544178842985	25.375708936360702	25.43938296648945	24.369466308719996
19	25.605384799401175	25.08713579808674	25.024693835029748	24.282785567482335
20	25.901362315821153	24.473972218685578	24.81104667986157	24.813618785631704
21	26.373092877439387	24.24308285272805	24.512701932988115	24.871122336844447
22	26.162629922454894	24.216349425003507	24.747036269603324	24.87398438293828
23	24.87470222690872	24.577588174590673	25.255273115389688	25.29243648311092
24	24.657117595048632	24.862695924764893	25.18357045253597	25.296616027650508
25	25.000980584428316	24.66391674355949	24.912502933715707	25.422599738296487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	332.5
3	499.0
4	499.0
5	499.0
6	988.5
7	1478.0
8	1478.0
9	1478.0
10	1830.5
11	2183.0
12	2183.0
13	2183.0
14	2970.5
15	3758.0
16	3758.0
17	3758.0
18	6340.5
19	8923.0
20	8923.0
21	8923.0
22	13612.0
23	18301.0
24	18301.0
25	18301.0
26	27028.0
27	35755.0
28	35755.0
29	35755.0
30	46398.5
31	57042.0
32	57042.0
33	57042.0
34	71561.5
35	86081.0
36	86081.0
37	86081.0
38	103928.0
39	121775.0
40	121775.0
41	121775.0
42	141870.5
43	161966.0
44	161966.0
45	161966.0
46	181082.5
47	200199.0
48	200199.0
49	200199.0
50	209297.0
51	218395.0
52	218395.0
53	218395.0
54	207538.5
55	196682.0
56	196682.0
57	196682.0
58	180930.5
59	165179.0
60	165179.0
61	165179.0
62	143793.5
63	122408.0
64	122408.0
65	122408.0
66	100212.0
67	78016.0
68	78016.0
69	78016.0
70	59122.5
71	40229.0
72	40229.0
73	40229.0
74	30062.5
75	19896.0
76	19896.0
77	19896.0
78	15256.0
79	10616.0
80	10616.0
81	10616.0
82	7251.0
83	3886.0
84	3886.0
85	3886.0
86	2847.5
87	1809.0
88	1809.0
89	1809.0
90	1188.0
91	567.0
92	567.0
93	567.0
94	356.5
95	146.0
96	146.0
97	146.0
98	373.0
99	600.0
100	600.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011563998702262368
2	1.927333117043728E-4
3	8.99422121287073E-4
4	0.00899422121287073
5	0.03205797418016068
6	0.06328077067626907
7	0.1062602991863442
8	0.1083803656150923
9	0.1196873865684155
10	0.13330720726219117
11	0.14120927304207045
12	0.13349994057389555
13	0.12932405215363416
14	0.1168606313300847
15	0.13433511825794783
16	0.12052256425246778
17	0.12463420823549441
18	0.11313445397046684
19	0.09707334466176909
20	0.09052041206382043
21	0.08981372325423773
22	0.10208441076608278
23	0.08094799091583657
24	0.09186954524575103
25	0.08737243463931567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1556555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.5937830895085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11684336685508	41.00912653138671
2	14.135126796485665	15.433800925400751
3	4.624297506749748	7.573736850745521
4	2.0536939231118856	4.4847568228244805
5	1.0933768199394374	2.9845788471435113
6	0.6544871046426236	2.1438556215443905
7	0.4382610493243346	1.6748430064375177
8	0.30648992820159554	1.3385955727485552
9	0.23601852415541402	1.1596629701561936
>10	1.210226713580201	11.725106845510737
>50	0.07512510183373383	2.853745503130046
>100	0.05132306292220421	5.490018121502306
>500	0.004020586868354312	1.5350084851872248
>1k	7.095153297095844E-4	0.5931638962821307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2703	0.1736527138456399	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1744	0.11204229853747538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2848887446958188E-4
2	0.0	0.0	0.0	0.0	1.2848887446958188E-4
3	0.0	0.0	0.0	0.0	1.2848887446958188E-4
4	0.0	0.0	0.0	0.0	1.927333117043728E-4
5	0.0	0.0	0.0	0.0	1.927333117043728E-4
6	0.0	0.0	0.0	0.0	3.854666234087456E-4
7	0.0	0.0	0.0	0.0	3.854666234087456E-4
8	0.0	6.424443723479094E-5	0.0	0.0	3.854666234087456E-4
9	0.0	6.424443723479094E-5	0.0	0.0	3.854666234087456E-4
10	0.0	1.2848887446958188E-4	0.0	0.0	3.854666234087456E-4
11	0.0	1.2848887446958188E-4	0.0	0.0	3.854666234087456E-4
12	0.0	1.2848887446958188E-4	0.0	0.0	5.139554978783275E-4
13	0.0	1.927333117043728E-4	0.0	0.0	5.781999351131184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	585	0.0	14.771351	1
GTACGCT	40	0.0052819503	14.248842	3
GTATCAA	2035	0.0	13.812173	1
ACCGTTA	100	1.00499165E-8	13.301914	8
CGCGTAA	80	1.9961735E-6	13.06438	10
TCGCGTA	90	5.391321E-7	12.669306	9
CGATAAC	75	1.4763971E-5	12.66849	10
ACGAACG	70	1.0907458E-4	12.214472	15
TTAGGAC	210	0.0	12.213293	3
TACGGCC	110	3.8262442E-8	12.087983	19
GTATTAG	110	3.8366124E-8	12.08565	1
TAGGACG	135	3.7107384E-10	11.963145	4
CCGTTAT	120	9.980795E-9	11.877473	9
GTCCTAT	185	0.0	11.805675	1
GCGTAAC	90	7.4431737E-6	11.61353	11
TCGAACT	75	2.0784838E-4	11.397241	19
CGGTTTT	60	0.0058735884	11.083858	14
GCCTAAT	60	0.005894718	11.078513	1
GGCCTAA	95	1.3666588E-5	10.995215	1
ATAACGA	70	0.0014896131	10.8587055	12
>>END_MODULE