##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1556555
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29641419673574	32.0	32.0	32.0	32.0	32.0
2	31.48716299777393	32.0	32.0	32.0	32.0	32.0
3	31.596263543530423	32.0	32.0	32.0	32.0	32.0
4	31.66656173408585	32.0	32.0	32.0	32.0	32.0
5	31.644513685671242	32.0	32.0	32.0	32.0	32.0
6	35.2959760496738	36.0	36.0	36.0	36.0	36.0
7	35.30427771585328	36.0	36.0	36.0	36.0	36.0
8	35.25878237518109	36.0	36.0	36.0	36.0	36.0
9	35.33385264253432	36.0	36.0	36.0	36.0	36.0
10	35.22929353604595	36.0	36.0	36.0	36.0	36.0
11	35.337051373064234	36.0	36.0	36.0	36.0	36.0
12	35.26996219214869	36.0	36.0	36.0	36.0	36.0
13	35.29747422995011	36.0	36.0	36.0	36.0	36.0
14	35.26168044174475	36.0	36.0	36.0	36.0	36.0
15	35.240153415716115	36.0	36.0	36.0	36.0	36.0
16	35.2528622502899	36.0	36.0	36.0	36.0	36.0
17	35.23275759610165	36.0	36.0	36.0	36.0	36.0
18	35.23214213439294	36.0	36.0	36.0	36.0	36.0
19	35.22306054074543	36.0	36.0	36.0	36.0	36.0
20	35.22176601533515	36.0	36.0	36.0	36.0	36.0
21	35.21682047855681	36.0	36.0	36.0	36.0	36.0
22	35.20117952786763	36.0	36.0	36.0	36.0	36.0
23	35.16873865684155	36.0	36.0	36.0	36.0	36.0
24	35.161164237691565	36.0	36.0	36.0	36.0	36.0
25	35.14437973601961	36.0	36.0	36.0	36.0	36.0
26	35.09826122430624	36.0	36.0	36.0	36.0	36.0
27	35.08576054170909	36.0	36.0	36.0	36.0	36.0
28	35.07120789178667	36.0	36.0	36.0	36.0	36.0
29	35.050465932781044	36.0	36.0	36.0	36.0	36.0
30	35.03891928007684	36.0	36.0	36.0	36.0	36.0
31	35.03549183935036	36.0	36.0	36.0	36.0	36.0
32	35.00953323204127	36.0	36.0	36.0	36.0	36.0
33	34.9841341937805	36.0	36.0	36.0	36.0	36.0
34	34.98236747175654	36.0	36.0	36.0	36.0	36.0
35	34.971205000786995	36.0	36.0	36.0	36.0	36.0
36	34.951342548127116	36.0	36.0	36.0	36.0	36.0
37	34.93619884938213	36.0	36.0	36.0	36.0	36.0
38	34.928275582937964	36.0	36.0	36.0	36.0	36.0
39	34.90782400878864	36.0	36.0	36.0	36.0	36.0
40	34.895300198194086	36.0	36.0	36.0	36.0	36.0
41	34.86286446672299	36.0	36.0	36.0	32.0	36.0
42	34.84090828785362	36.0	36.0	36.0	32.0	36.0
43	34.83845543524	36.0	36.0	36.0	32.0	36.0
44	34.79675565591964	36.0	36.0	36.0	32.0	36.0
45	34.78373973293588	36.0	36.0	36.0	32.0	36.0
46	34.76426660156564	36.0	36.0	36.0	32.0	36.0
47	34.74230913780753	36.0	36.0	36.0	32.0	36.0
48	34.715019385758936	36.0	36.0	36.0	32.0	36.0
49	34.703374439065755	36.0	36.0	36.0	32.0	36.0
50	34.29977675058061	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	15.0
21	30.0
22	93.0
23	259.0
24	766.0
25	1623.0
26	3426.0
27	6643.0
28	11274.0
29	18018.0
30	26937.0
31	40119.0
32	61272.0
33	104054.0
34	241563.0
35	1040459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14001498654938	17.68354988425911	12.07136990685461	26.105065222336897
2	16.621179179580487	19.184154935006863	37.16976598358339	27.024899901829265
3	19.196017865127537	22.361218899207994	27.973109796524625	30.469653439139844
4	13.10015585710486	15.225170472723724	34.09351683269581	37.5811568374756
5	15.00223249419391	36.13704623350926	32.7466103028804	16.114110969416437
6	36.03177795009608	34.01051427161719	15.85396173976953	14.10374603851721
7	31.801703120031092	29.636151629720764	19.151780695189053	19.41036455505909
8	28.69554882416619	33.315880261217885	18.031421954251535	19.957148960364393
9	26.943731509647904	14.751936166727164	18.42299179919759	39.88134052442734
10	16.734669490855755	26.726555408007307	30.26797031646208	26.270804784674855
11	37.58453764884633	21.357292225459428	21.21396288598861	19.844207239705632
12	24.87666674161851	23.383176309221327	27.945944730510647	23.79421221864952
13	29.82779278599214	19.31907320974845	24.7909646623473	26.062169341912107
14	23.617475771816608	19.322735142670833	24.55242506689452	32.50736401861804
15	25.384519017959533	26.997054392552783	21.782590399953744	25.83583618953394
16	26.0823169262694	25.915408806041583	23.008393589648872	24.99388067804015
17	24.458628188531726	25.984240839546306	24.62534250315601	24.931788468765962
18	25.039542476521852	25.21011156696138	25.52298217729677	24.227363779219996
19	25.830311167931747	24.901208116642202	25.050383699901385	24.218097015524666
20	26.125114997764285	24.380232719497968	24.763965482682234	24.73068680005551
21	26.621288679166494	24.063396410663294	24.474689297840424	24.84062561232979
22	26.210124280863827	24.115498649260708	24.830731968995636	24.843645100879826
23	24.9787029553745	24.258972062646077	25.3411703031183	25.421154678861125
24	24.801722803178603	24.70188553663816	25.201842813483555	25.294548846699684
25	25.214079076111222	24.3506316208078	25.076788360933882	25.358500942147096
26	24.958385558841307	25.054688217732668	25.23470159345887	24.752224629967152
27	25.360814675046754	24.707830858946117	24.968086411781975	24.963268054225153
28	25.196190231445048	24.534524019851915	25.149612116734392	25.11967363196865
29	24.843291152414732	24.811233054661304	25.302255181173223	25.043220611750737
30	24.92129940148998	24.95907568877479	25.343841308755614	24.775783600979615
31	25.35304196543237	24.595915435680222	24.768477324429842	25.282565274457564
32	25.271387353609516	24.60497295602616	24.75382884720524	25.369810843159087
33	24.82909351625033	24.63436837679154	25.012190397628608	25.524347709329525
34	25.48966070192999	24.65890627720382	25.006279966361223	24.84515305450497
35	25.551717292549093	24.41747738105257	25.362259716847973	24.668545609550357
36	24.82197786383716	24.867977087760863	25.044136126865347	25.265908921536628
37	25.70463231784216	24.55482450927421	24.783796685043118	24.956746487840512
38	25.008319659966826	24.554753641698266	25.081044409638213	25.355882288696698
39	25.621218370511993	24.5258387524108	24.63075106422073	25.222191812856483
40	25.566090222375838	24.678616996262257	24.907648562144328	24.847644219217578
41	24.88419754196835	24.920239250126887	25.302756773078578	24.892806434826184
42	25.595096596786316	24.888112202612323	24.86286275996325	24.653928440638108
43	25.42470886193059	24.286048373314625	24.953581097837553	25.335661666917233
44	24.998586557908723	24.8860251695488	24.901316043081714	25.21407222946076
45	25.223736570987292	24.956987326841066	24.807294229812953	25.011981872358685
46	25.110182536806754	24.64722314308696	24.99620946374834	25.24638485635795
47	25.18009553270566	24.55363997597227	25.283337669888567	24.982926821433505
48	25.368089468371963	25.13660605351938	24.51173575297642	24.983568725132237
49	25.197220696367822	24.954947141191322	24.640139286414115	25.207692876026737
50	25.108944604166332	25.241162204269123	24.56510496137229	25.084788230192256
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	66.0
2	66.0
3	164.5
4	263.0
5	272.5
6	282.0
7	220.5
8	159.0
9	221.5
10	284.0
11	512.0
12	740.0
13	1335.5
14	1931.0
15	2416.0
16	2901.0
17	2806.5
18	2712.0
19	2680.5
20	2649.0
21	3099.5
22	3550.0
23	4284.5
24	5019.0
25	6394.5
26	7770.0
27	9803.5
28	11837.0
29	13980.5
30	16124.0
31	19112.0
32	22100.0
33	25704.0
34	29308.0
35	34116.5
36	38925.0
37	44675.0
38	50425.0
39	56481.0
40	62537.0
41	68478.0
42	74419.0
43	79858.5
44	85298.0
45	93570.0
46	101842.0
47	108166.0
48	114490.0
49	119940.0
50	125390.0
51	124368.5
52	123347.0
53	122945.0
54	122543.0
55	120561.5
56	118580.0
57	113628.0
58	108676.0
59	100802.0
60	92928.0
61	81360.0
62	69792.0
63	60715.0
64	51638.0
65	43634.0
66	35630.0
67	30916.0
68	26202.0
69	22265.5
70	18329.0
71	14270.5
72	10212.0
73	8635.5
74	7059.0
75	5468.0
76	3877.0
77	3284.5
78	2692.0
79	2234.0
80	1776.0
81	1384.5
82	993.0
83	810.0
84	627.0
85	492.5
86	358.0
87	237.0
88	116.0
89	81.5
90	47.0
91	30.0
92	13.0
93	10.0
94	7.0
95	7.5
96	8.0
97	8.5
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03167250755675193
2	0.005332288290487648
3	1.927333117043728E-4
4	6.424443723479094E-5
5	0.0
6	2.5697774893916375E-4
7	0.0
8	0.0
9	0.0
10	1.2848887446958188E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.424443723479093E-4
17	0.0
18	6.424443723479094E-5
19	0.0
20	1.927333117043728E-4
21	0.0
22	0.0
23	6.424443723479094E-5
24	0.0014133776191654005
25	2.5697774893916375E-4
26	2.5697774893916375E-4
27	5.139554978783275E-4
28	0.0019273331170437278
29	3.854666234087456E-4
30	0.0014776220564001914
31	7.709332468174912E-4
32	0.001027910995756655
33	1.2848887446958188E-4
34	0.0011563998702262368
35	3.854666234087456E-4
36	4.497110606435365E-4
37	0.002184310865982892
38	6.424443723479094E-5
39	0.001092155432991446
40	6.424443723479094E-5
41	0.0016061109308697734
42	0.005460777164957229
43	0.004946821667078902
44	0.0045613550436701565
45	0.0023770441776872647
46	0.0030194885500351735
47	0.0012848887446958186
48	0.004625599480904947
49	0.003212221861739547
50	0.0019273331170437278
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1556555.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.099951917896796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90116083355682	42.019515213571914
2	14.509923184561933	16.280119859726007
3	4.696965549143452	7.9049862440089615
4	1.9767206094500172	4.435757245810464
5	1.0741345645317102	3.012944871178997
6	0.6363277505363184	2.141877372546653
7	0.41967785990304424	1.6480735433096625
8	0.30128934012750247	1.3521853995622193
9	0.2198972969274265	1.110260500605369
>10	1.1493053868487184	11.27343197362342
>50	0.06822970012559558	2.6314573507227537
>100	0.04267709272721041	4.579922351227914
>500	0.0031141391290018484	1.242203428748138
>1k	5.766924312966386E-4	0.3672646453575597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.424443723479094E-5
7	0.0	0.0	0.0	0.0	6.424443723479094E-5
8	0.0	0.0	0.0	0.0	6.424443723479094E-5
9	0.0	0.0	0.0	0.0	6.424443723479094E-5
10	0.0	0.0	0.0	0.0	6.424443723479094E-5
11	0.0	0.0	0.0	0.0	6.424443723479094E-5
12	0.0	0.0	0.0	0.0	1.2848887446958188E-4
13	0.0	0.0	0.0	6.424443723479094E-5	1.927333117043728E-4
14	0.0	0.0	0.0	1.2848887446958188E-4	1.927333117043728E-4
15	0.0	0.0	0.0	1.2848887446958188E-4	3.854666234087456E-4
16	0.0	0.0	0.0	1.2848887446958188E-4	3.854666234087456E-4
17	0.0	0.0	0.0	1.2848887446958188E-4	3.854666234087456E-4
18	0.0	0.0	0.0	1.927333117043728E-4	3.854666234087456E-4
19	0.0	0.0	0.0	3.854666234087456E-4	3.854666234087456E-4
20	0.0	0.0	0.0	7.066888095827003E-4	4.497110606435365E-4
21	0.0	0.0	0.0	0.001027910995756655	4.497110606435365E-4
22	0.0	0.0	0.0	0.0012848887446958186	4.497110606435365E-4
23	0.0	0.0	0.0	0.0017345998053393552	4.497110606435365E-4
24	0.0	0.0	0.0	0.0021200664287481007	4.497110606435365E-4
25	0.0	0.0	0.0	0.0026982663638612193	4.497110606435365E-4
26	0.0	0.0	0.0	0.0030837329872699646	5.139554978783275E-4
27	0.0	0.0	0.0	0.0036619329223830832	5.781999351131184E-4
28	0.0	0.0	0.0	0.004497110606435365	5.781999351131184E-4
29	0.0	0.0	0.0	0.006745665909653048	5.781999351131184E-4
30	0.0	0.0	0.0	0.010086376645862176	5.781999351131184E-4
31	0.0	6.424443723479094E-5	0.0	0.01644657593210648	5.781999351131184E-4
32	0.0	6.424443723479094E-5	0.0	0.024477130586455346	5.781999351131184E-4
33	0.0	6.424443723479094E-5	0.0	0.03334286292485649	5.781999351131184E-4
34	0.0	1.2848887446958188E-4	0.0	0.042851039635605555	6.424443723479093E-4
35	0.0	1.2848887446958188E-4	0.0	0.0517167719740067	6.424443723479093E-4
36	0.0	1.2848887446958188E-4	0.0	0.065143859356078	6.424443723479093E-4
37	0.0	1.2848887446958188E-4	0.0	0.08602330145738506	6.424443723479093E-4
38	0.0	1.2848887446958188E-4	0.0	0.10960100992255332	6.424443723479093E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	945	0.0	33.531277	1
GTATCAA	2310	0.0	25.815271	1
ATACCGT	315	0.0	21.65005	6
TAGGACG	170	0.0	20.705172	4
CGCAATA	235	0.0	20.5957	36
ACCGTCG	310	0.0	19.870285	8
GTGTTAT	135	2.59206E-9	19.559912	1
TACCGTC	360	0.0	18.943794	7
TATGCGC	85	1.4315438E-4	18.117025	10
GTCCTAA	300	0.0	17.60392	1
TATCAAC	3350	0.0	17.599396	2
CGTCGTA	325	0.0	17.599396	10
TACACCG	90	2.2164677E-4	17.110523	5
TCAACGC	3440	0.0	16.819191	4
ATCAACG	3480	0.0	16.56265	3
CGCAACT	120	6.41101E-6	16.499434	15
CCGTCGT	365	0.0	16.273415	9
CAACGCA	3575	0.0	16.122522	5
ACGCAAC	110	5.6245637E-5	15.999451	14
AACGCAG	3635	0.0	15.856402	6
>>END_MODULE