FastQCFastQC Report
Thu 2 Feb 2017
SRR4062002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852016
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14070.16513774389213348No Hit
TATCAACGCAGAGTACTTTTTTTTT8830.1036365514262643No Hit
GCGCAAGACGGACCAGAGCGAAAGC8690.1019933897954968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAT350.002167636816.28737613
CCGATAA508.6946246E-515.2024449
TCGCGTA508.6946246E-515.2024449
TAGATCT602.5630989E-514.2514544
ATTATAC602.5687867E-514.2481063
CCCCTAG400.0052955914.2422481
CGATAAC551.954867E-413.82121710
TTCCGAT551.955842E-413.8204047
AAGACGG1750.013.5728145
CAAGACG1750.013.5728144
GTATTAA707.294204E-613.5640461
ATTCCGA655.4356206E-513.1567356
CGGTCGG655.4356206E-513.15673510
GTATGGA655.4747165E-513.1466911
GCGCAAG1950.013.146691
GCGGTCG801.9927684E-613.0646019
ACTGCGC604.0831012E-412.6687048
CCGTCGT1051.9841536E-812.6687039
TAATTCC1352.7284841E-1112.6679594
GCGTAAC604.0851263E-412.66795911
Produced by FastQC (version 0.11.5)