FastQCFastQC Report
Thu 2 Feb 2017
SRR4062002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852016
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8800.1032844453625284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4950.028.0125561
GTATCAA10400.024.7610991
TAGCGGC1900.024.31527130
CGCAATA2150.023.5343836
CTAGCGG2050.022.53610429
GCGCAAT2250.021.51065335
TCTAGCG2200.020.99955228
CAATACG2350.020.59530438
CGACGGT2600.020.3072597
ATACGAA2500.019.35958940
GCAATAC2650.019.09393337
TACGAAT2450.018.8567441
GGCGTTA1759.094947E-1218.85673942
GACGGTA2800.018.8567398
CACGTTT708.1218034E-418.85673928
AATACGA2600.018.61498839
ACGGTAT2850.018.525929
GCCGGTC2500.018.4796078
CGCCGGT2500.018.4796077
TACGACG2900.018.2065075
Produced by FastQC (version 0.11.5)