##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852016
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28419771459691	32.0	32.0	32.0	32.0	32.0
2	31.460204972676568	32.0	32.0	32.0	32.0	32.0
3	31.580559520009015	32.0	32.0	32.0	32.0	32.0
4	31.652472488779555	32.0	32.0	32.0	32.0	32.0
5	31.632207611124674	32.0	32.0	32.0	32.0	32.0
6	35.27527534694184	36.0	36.0	36.0	36.0	36.0
7	35.27541032093294	36.0	36.0	36.0	36.0	36.0
8	35.2307480141218	36.0	36.0	36.0	36.0	36.0
9	35.309664372500045	36.0	36.0	36.0	36.0	36.0
10	35.202433991849915	36.0	36.0	36.0	36.0	36.0
11	35.31625345063942	36.0	36.0	36.0	36.0	36.0
12	35.23666809074008	36.0	36.0	36.0	36.0	36.0
13	35.271581754333255	36.0	36.0	36.0	36.0	36.0
14	35.23758708756643	36.0	36.0	36.0	36.0	36.0
15	35.21592904358604	36.0	36.0	36.0	36.0	36.0
16	35.23125504685358	36.0	36.0	36.0	36.0	36.0
17	35.21004065651349	36.0	36.0	36.0	36.0	36.0
18	35.2077695724024	36.0	36.0	36.0	36.0	36.0
19	35.1979552027192	36.0	36.0	36.0	36.0	36.0
20	35.20043285572102	36.0	36.0	36.0	36.0	36.0
21	35.1921372368594	36.0	36.0	36.0	36.0	36.0
22	35.167291459315315	36.0	36.0	36.0	36.0	36.0
23	35.138147640419895	36.0	36.0	36.0	36.0	36.0
24	35.12202470376143	36.0	36.0	36.0	36.0	36.0
25	35.12012802576477	36.0	36.0	36.0	36.0	36.0
26	35.06946231056694	36.0	36.0	36.0	36.0	36.0
27	35.06293778520591	36.0	36.0	36.0	36.0	36.0
28	35.042165874819254	36.0	36.0	36.0	36.0	36.0
29	35.01588233084825	36.0	36.0	36.0	36.0	36.0
30	35.001497624457755	36.0	36.0	36.0	36.0	36.0
31	35.007808538806785	36.0	36.0	36.0	36.0	36.0
32	34.9807198456367	36.0	36.0	36.0	36.0	36.0
33	34.962396246079884	36.0	36.0	36.0	36.0	36.0
34	34.95404663762183	36.0	36.0	36.0	36.0	36.0
35	34.942381363730256	36.0	36.0	36.0	36.0	36.0
36	34.918738615237274	36.0	36.0	36.0	36.0	36.0
37	34.90583510168823	36.0	36.0	36.0	36.0	36.0
38	34.893615847589714	36.0	36.0	36.0	32.0	36.0
39	34.886449315505814	36.0	36.0	36.0	32.0	36.0
40	34.86678536553304	36.0	36.0	36.0	32.0	36.0
41	34.83755234643481	36.0	36.0	36.0	32.0	36.0
42	34.80974066214719	36.0	36.0	36.0	32.0	36.0
43	34.80496962498357	36.0	36.0	36.0	32.0	36.0
44	34.769486723254026	36.0	36.0	36.0	32.0	36.0
45	34.750167837223714	36.0	36.0	36.0	32.0	36.0
46	34.73499676062421	36.0	36.0	36.0	32.0	36.0
47	34.71121903814013	36.0	36.0	36.0	32.0	36.0
48	34.69745990685621	36.0	36.0	36.0	32.0	36.0
49	34.67196742784173	36.0	36.0	36.0	32.0	36.0
50	34.27674362922762	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	29.0
22	61.0
23	166.0
24	429.0
25	954.0
26	1893.0
27	3632.0
28	6440.0
29	10225.0
30	15265.0
31	22503.0
32	34754.0
33	58845.0
34	136191.0
35	560624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89929191092472	17.929998861140103	12.044323957502915	26.126385270432262
2	16.368438041022333	19.65221984213152	37.20977728219725	26.769564834648907
3	18.885297390177602	22.877494683173477	28.301009844931762	29.93619808171716
4	12.949052599951175	15.77892903419654	34.730568440029295	36.54144992582299
5	15.04267525492479	36.21598655424311	32.92344275224878	15.817895438583312
6	35.47891119415598	34.348885466939585	16.24312219488836	13.929081144016076
7	31.151058196090215	29.913405382058556	19.74387804923851	19.19165837261272
8	28.1921935738296	33.39796435747686	18.739436818087924	19.670405250605622
9	26.84010628908377	14.683761807290004	18.648593453644065	39.82753844998216
10	16.90695949371843	26.530839796435746	30.512689902537044	26.049510807308785
11	37.50504685358022	21.223897203808377	21.602528591012376	19.66852735159903
12	24.62770652194325	23.519041895926836	28.147828209798874	23.705423372331037
13	29.594984131753392	19.254098514581884	24.951409363204448	26.199507990460276
14	23.600026290586094	19.437545773788287	24.800238493173836	32.16218944245178
15	25.27100430038873	27.078247356857148	21.8382049163396	25.812543426414525
16	26.01254917794199	25.77640116055248	23.352597628191283	24.85845203331424
17	24.28827627650185	26.054675029576906	24.80587219019361	24.85117650372763
18	25.099000720645435	25.188318501808656	25.53209219566815	24.180588581877764
19	25.650252636397248	25.145214579555525	25.02937154862297	24.17516123542426
20	25.9123676371515	24.4749499421371	24.739382216724138	24.87330020398726
21	26.422860603556742	24.36362697414133	24.598833824716905	24.614678597585023
22	26.065707686240636	24.235460366941467	24.840261215751816	24.85857073106608
23	24.59543013276746	24.399893898706125	25.417245685527035	25.587430282999378
24	24.88201415712745	24.835183665900242	25.211470770848663	25.071331406123644
25	24.99322191849636	24.399567611216288	25.233124924443462	25.374085545843894
26	24.736593158053495	25.35736124853141	25.342924781310995	24.563120812104096
27	25.385615729319	24.755461764712784	24.826939463436045	25.031983042532165
28	25.07367949098651	24.661708143535044	25.303491427826962	24.961120937651483
29	24.74803729059836	24.948973663485567	25.36739549137276	24.93559355454331
30	24.776203839657843	24.97573365352	25.4162250602404	24.831837446581762
31	25.3625238112564	24.73272121434767	24.69739311489379	25.207361859502143
32	25.09301534019554	24.858980528397552	24.662034483163342	25.385969648243567
33	24.72776141125104	24.621542000680744	25.174469783218505	25.47622680484971
34	25.3575364637311	24.684775196564363	25.296269649100772	24.66141869060376
35	25.779358344630083	24.490413901163713	25.294742463454284	24.435485290751924
36	24.635364400861487	24.985710345475137	25.175613105403073	25.2033121482603
37	25.52681801216429	24.779049814437055	24.957335780901925	24.736796392496732
38	24.864820455038938	24.652382880583087	25.31223041847855	25.17056624589943
39	25.488608141669367	24.57911390919377	24.81878189509068	25.113496054046188
40	25.73358450262026	24.683485619384633	24.978433478283833	24.604496399711273
41	24.773445101590255	25.14797517843289	25.37356176811999	24.705017951856863
42	25.65086392568866	25.108130091797605	24.980662759702952	24.260343222810786
43	25.316289294814837	24.32672252810776	25.111474443918873	25.245513733158525
44	24.908243474341624	24.866928095983134	24.940403740450222	25.28442468922502
45	25.050293545290014	25.03221838548917	24.93808670912373	24.97940136009709
46	24.97030516431925	24.91349765258216	24.960680751173708	25.155516431924884
47	25.046214517520436	24.820746357122317	25.31827864859948	24.814760476757765
48	25.61743972318794	25.26086109396164	24.449580157420428	24.672119025429996
49	24.992429488609023	25.335626005000062	24.61073486778014	25.061209638610784
50	25.116725078110147	25.425292428891012	24.65358062539759	24.80440186760125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	165.0
2	35.0
3	87.0
4	139.0
5	131.0
6	123.0
7	101.0
8	79.0
9	121.5
10	164.0
11	277.0
12	390.0
13	722.5
14	1055.0
15	1306.0
16	1557.0
17	1517.5
18	1478.0
19	1449.0
20	1420.0
21	1721.5
22	2023.0
23	2488.5
24	2954.0
25	3821.5
26	4689.0
27	5862.0
28	7035.0
29	8318.5
30	9602.0
31	11272.0
32	12942.0
33	14954.0
34	16966.0
35	19633.5
36	22301.0
37	25843.0
38	29385.0
39	33179.0
40	36973.0
41	39766.0
42	42559.0
43	44994.0
44	47429.0
45	51406.5
46	55384.0
47	59408.5
48	63433.0
49	65745.5
50	68058.0
51	66619.5
52	65181.0
53	64447.5
54	63714.0
55	63833.0
56	63952.0
57	61455.0
58	58958.0
59	53925.5
60	48893.0
61	43176.0
62	37459.0
63	32049.5
64	26640.0
65	22524.0
66	18408.0
67	15955.0
68	13502.0
69	11847.0
70	10192.0
71	7966.0
72	5740.0
73	5005.5
74	4271.0
75	3237.0
76	2203.0
77	1966.0
78	1729.0
79	1471.0
80	1213.0
81	935.5
82	658.0
83	543.5
84	429.0
85	341.5
86	254.0
87	168.5
88	83.0
89	58.0
90	33.0
91	23.5
92	14.0
93	11.0
94	8.0
95	7.0
96	6.0
97	4.5
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03368481343073369
2	0.004812116204390528
3	4.6947475164785637E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.389495032957127E-4
17	0.0
18	2.3473737582392818E-4
19	1.1736868791196409E-4
20	2.3473737582392818E-4
21	0.0
22	0.0
23	0.0
24	8.215808153837487E-4
25	5.868434395598205E-4
26	8.215808153837487E-4
27	2.3473737582392818E-4
28	0.0017605303186794615
29	5.868434395598205E-4
30	0.0015257929428555332
31	3.521060637358923E-4
32	7.042121274717846E-4
33	7.042121274717846E-4
34	5.868434395598205E-4
35	1.1736868791196409E-4
36	1.1736868791196409E-4
37	0.0016431616307674974
38	1.1736868791196409E-4
39	4.6947475164785637E-4
40	1.1736868791196409E-4
41	0.0017605303186794615
42	0.003873166701094815
43	0.0031689545736230305
44	0.003873166701094815
45	0.002112636382415354
46	0.0018778990065914255
47	0.001291055567031605
48	0.003521060637358923
49	0.0030515858857110664
50	0.0016431616307674974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	852016.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.97048798669466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.40295608717692	54.36163224617232
2	11.574312337718393	15.271260660594061
3	2.864958225783315	5.670080766492607
4	1.0972168895539682	2.8953573452447414
5	0.5583338901252475	1.841677959553607
6	0.3307361158782344	1.309129377558667
7	0.23033270081816185	1.063661247058724
8	0.16813837262611836	0.8873736393146986
9	0.11090133398440435	0.6584593609193904
>10	0.5592093200466745	6.730641152365309
>50	0.05575741305304878	2.5420305025214236
>100	0.0442790356679022	5.560397110498203
>500	0.0028682775676067357	1.2082986317063735
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	880	0.1032844453625284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1736868791196409E-4	0.0	0.0	0.0
6	0.0	1.1736868791196409E-4	0.0	0.0	0.0
7	0.0	1.1736868791196409E-4	0.0	0.0	0.0
8	0.0	1.1736868791196409E-4	0.0	0.0	0.0
9	0.0	1.1736868791196409E-4	0.0	0.0	0.0
10	0.0	1.1736868791196409E-4	0.0	0.0	0.0
11	0.0	1.1736868791196409E-4	0.0	0.0	0.0
12	0.0	1.1736868791196409E-4	0.0	0.0	0.0
13	0.0	1.1736868791196409E-4	0.0	0.0	0.0
14	0.0	1.1736868791196409E-4	0.0	0.0	0.0
15	0.0	1.1736868791196409E-4	0.0	0.0	0.0
16	0.0	1.1736868791196409E-4	0.0	0.0	0.0
17	0.0	1.1736868791196409E-4	0.0	0.0	0.0
18	0.0	1.1736868791196409E-4	0.0	2.3473737582392818E-4	0.0
19	0.0	1.1736868791196409E-4	0.0	2.3473737582392818E-4	0.0
20	0.0	1.1736868791196409E-4	0.0	2.3473737582392818E-4	0.0
21	0.0	1.1736868791196409E-4	0.0	3.521060637358923E-4	0.0
22	0.0	1.1736868791196409E-4	0.0	3.521060637358923E-4	0.0
23	0.0	1.1736868791196409E-4	0.0	5.868434395598205E-4	0.0
24	0.0	1.1736868791196409E-4	0.0	5.868434395598205E-4	0.0
25	0.0	1.1736868791196409E-4	0.0	5.868434395598205E-4	0.0
26	0.0	1.1736868791196409E-4	0.0	0.0010563181912076767	0.0
27	0.0	1.1736868791196409E-4	0.0	0.001291055567031605	2.3473737582392818E-4
28	0.0	1.1736868791196409E-4	0.0	0.002230005070327318	2.3473737582392818E-4
29	0.0	1.1736868791196409E-4	0.0	0.003521060637358923	2.3473737582392818E-4
30	0.0	1.1736868791196409E-4	0.0	0.006337909147246061	2.3473737582392818E-4
31	0.0	1.1736868791196409E-4	0.0	0.00974160109669302	2.3473737582392818E-4
32	0.0	1.1736868791196409E-4	0.0	0.015844772868115153	2.3473737582392818E-4
33	0.0	1.1736868791196409E-4	0.0	0.022769525454921034	2.3473737582392818E-4
34	0.0	1.1736868791196409E-4	0.0	0.02840322247469531	3.521060637358923E-4
35	0.0	1.1736868791196409E-4	0.0	0.03614955587688494	3.521060637358923E-4
36	0.0	1.1736868791196409E-4	0.0	0.048473268107641174	3.521060637358923E-4
37	0.0	1.1736868791196409E-4	0.0	0.06736962686146739	3.521060637358923E-4
38	0.0	1.1736868791196409E-4	0.0	0.08943494018891664	3.521060637358923E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	495	0.0	28.012556	1
GTATCAA	1040	0.0	24.761099	1
TAGCGGC	190	0.0	24.315271	30
CGCAATA	215	0.0	23.53438	36
CTAGCGG	205	0.0	22.536104	29
GCGCAAT	225	0.0	21.510653	35
TCTAGCG	220	0.0	20.999552	28
CAATACG	235	0.0	20.595304	38
CGACGGT	260	0.0	20.307259	7
ATACGAA	250	0.0	19.359589	40
GCAATAC	265	0.0	19.093933	37
TACGAAT	245	0.0	18.85674	41
GGCGTTA	175	9.094947E-12	18.856739	42
GACGGTA	280	0.0	18.856739	8
CACGTTT	70	8.1218034E-4	18.856739	28
AATACGA	260	0.0	18.614988	39
ACGGTAT	285	0.0	18.52592	9
GCCGGTC	250	0.0	18.479607	8
CGCCGGT	250	0.0	18.479607	7
TACGACG	290	0.0	18.206507	5
>>END_MODULE