FastQCFastQC Report
Thu 2 Feb 2017
SRR4062001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402651
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22680.16169382119999914No Hit
TATCAACGCAGAGTACTTTTTTTTT15100.10765329365608409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG350.002169646816.28617313
TATGCCG456.756634E-414.7792495
GGTATCA6250.014.585121
CGTTATT707.2726034E-613.5693882
AGTCGGT500.001497890313.30186
TCGCGTA801.9959643E-613.0642689
GTACCGC604.0869074E-412.6683826
GCGTAAC853.9349925E-612.29622211
TAGGACG550.003067536312.091254
GTTTAGA951.0454551E-611.9943421
CACCGTG1209.988071E-911.8766087
GCGTTAT802.8797522E-511.86941
TGCACCG658.015849E-411.6934735
GTATCAA14450.011.6311921
CGGTCGG752.0702442E-411.4019510
CGATAAC752.0702442E-411.4019510
CGCGTAA855.312489E-511.17838310
ACGGTAT1451.2132659E-911.1394399
CCGACCT600.005869250311.0848349
CGGCGTC600.00587705611.08285414
Produced by FastQC (version 0.11.5)