##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062001_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1402651
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300961536404994	32.0	32.0	32.0	32.0	32.0
2	31.021532797538377	32.0	32.0	32.0	32.0	32.0
3	31.036104490710805	32.0	32.0	32.0	32.0	32.0
4	31.029601804012543	32.0	32.0	32.0	32.0	32.0
5	31.008838264115592	32.0	32.0	32.0	32.0	32.0
6	34.55719990218522	36.0	36.0	36.0	32.0	36.0
7	34.52969911973827	36.0	36.0	36.0	32.0	36.0
8	34.51669873689178	36.0	36.0	36.0	32.0	36.0
9	34.604471818007475	36.0	36.0	36.0	32.0	36.0
10	34.425423002585816	36.0	36.0	36.0	32.0	36.0
11	34.546275588154145	36.0	36.0	36.0	32.0	36.0
12	34.473670214472456	36.0	36.0	36.0	32.0	36.0
13	34.50840872034455	36.0	36.0	36.0	32.0	36.0
14	34.463187920587515	36.0	36.0	36.0	32.0	36.0
15	34.422609045300646	36.0	36.0	36.0	32.0	36.0
16	34.422425107885	36.0	36.0	36.0	32.0	36.0
17	34.37623542848506	36.0	36.0	36.0	32.0	36.0
18	34.368700410864854	36.0	36.0	36.0	32.0	36.0
19	34.36231321975317	36.0	36.0	36.0	32.0	36.0
20	34.34914529701258	36.0	36.0	36.0	32.0	36.0
21	34.32951104729544	36.0	36.0	36.0	32.0	36.0
22	34.29823313140617	36.0	36.0	36.0	32.0	36.0
23	34.27335666534298	36.0	36.0	36.0	32.0	36.0
24	34.25053416708789	36.0	36.0	36.0	32.0	36.0
25	33.861395315014214	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	14.0
5	97.0
6	298.0
7	56.0
8	244.0
9	179.0
10	104.0
11	22.0
12	62.0
13	57.0
14	185.0
15	274.0
16	477.0
17	723.0
18	901.0
19	1271.0
20	1669.0
21	2149.0
22	2935.0
23	4240.0
24	5963.0
25	8266.0
26	11646.0
27	15698.0
28	21514.0
29	30214.0
30	41367.0
31	59092.0
32	88900.0
33	135347.0
34	319547.0
35	649138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45857656583747	17.442444083853545	11.910574090498177	26.188405259810814
2	16.59310347777669	19.015805094503328	37.68476646687803	26.706324960841947
3	19.7107034999615	22.040802940731933	28.297344158603323	29.95114940070324
4	13.201247184530121	15.31551444222859	34.841167927136816	36.642070446104476
5	15.016345835663019	35.98308933441355	33.544051028675106	15.456513801248331
6	35.112102856841204	34.58392216254468	16.270091233156396	14.03388374745772
7	30.749500794304417	30.079944277368533	19.839269325576318	19.331285602750732
8	28.02230113960765	33.652753671180946	18.838211112887386	19.486734076324023
9	26.687022639238922	14.98183878954765	18.69038008997454	39.64075848123888
10	16.483528250287506	26.66182675841077	31.20919896662962	25.645446024672108
11	36.84466022882949	21.464026139703492	21.886576131458092	19.804737500008923
12	24.120897515791757	23.59380364896862	28.804280710320374	23.48101812491925
13	29.19364302550185	19.745623940873056	25.077270761029553	25.983462272595542
14	23.324478297549188	19.557257727220172	25.05806210325419	32.06020187197645
15	25.070401823987233	27.148442379720848	22.10235372303405	25.678802073257874
16	25.654877760671717	26.05828574446688	23.600794539574594	24.686041955286807
17	23.891962112506157	26.066638591281876	25.33972405227732	24.701675243934645
18	24.397478362462916	25.554239972365618	25.937281653038603	24.111000012132866
19	25.242199519882625	25.007706997097028	25.484113595428326	24.26597988759202
20	25.404081236544602	24.644049582235912	25.272354785145307	24.679514396074183
21	25.831120650839058	24.648866733124443	24.920454034598478	24.599558581438014
22	25.43666774428594	24.535035829056756	25.229279557990548	24.799016868666758
23	24.530029118323423	24.656463004888256	25.441894282148773	25.37161359463955
24	24.42930243278324	24.941576239172427	25.41802869846489	25.21109262957944
25	24.561527440033792	24.850798027194575	25.46316116952655	25.124513363245082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	50.0
2	202.5
3	355.0
4	355.0
5	355.0
6	800.5
7	1246.0
8	1246.0
9	1246.0
10	1597.0
11	1948.0
12	1948.0
13	1948.0
14	2835.5
15	3723.0
16	3723.0
17	3723.0
18	6225.5
19	8728.0
20	8728.0
21	8728.0
22	13897.0
23	19066.0
24	19066.0
25	19066.0
26	28351.0
27	37636.0
28	37636.0
29	37636.0
30	47423.5
31	57211.0
32	57211.0
33	57211.0
34	70957.5
35	84704.0
36	84704.0
37	84704.0
38	100970.0
39	117236.0
40	117236.0
41	117236.0
42	133942.5
43	150649.0
44	150649.0
45	150649.0
46	164832.0
47	179015.0
48	179015.0
49	179015.0
50	186830.5
51	194646.0
52	194646.0
53	194646.0
54	183596.5
55	172547.0
56	172547.0
57	172547.0
58	156859.0
59	141171.0
60	141171.0
61	141171.0
62	122198.5
63	103226.0
64	103226.0
65	103226.0
66	84215.5
67	65205.0
68	65205.0
69	65205.0
70	48966.0
71	32727.0
72	32727.0
73	32727.0
74	24652.5
75	16578.0
76	16578.0
77	16578.0
78	12768.0
79	8958.0
80	8958.0
81	8958.0
82	6295.0
83	3632.0
84	3632.0
85	3632.0
86	2516.5
87	1401.0
88	1401.0
89	1401.0
90	894.5
91	388.0
92	388.0
93	388.0
94	258.0
95	128.0
96	128.0
97	128.0
98	302.5
99	477.0
100	477.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010694035793650737
2	1.4258714391534317E-4
3	4.990550037037011E-4
4	0.009410751498412648
5	0.03272374952857125
6	0.06116988473968221
7	0.101593340039682
8	0.1045876700619042
9	0.11321419226878247
10	0.1281858423798935
11	0.13331897956084585
12	0.1256192737894173
13	0.12355176020264484
14	0.11157444011375602
15	0.12576186093333266
16	0.11321419226878247
17	0.1184186230216925
18	0.10679777079259202
19	0.09460656998783018
20	0.08997248781058154
21	0.08982990066666618
22	0.10080911074814762
23	0.08041914916825355
24	0.08925955209100482
25	0.08519581848941754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1402651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.669628556988165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.74491218250421	30.482153474437613
2	16.129845458285562	14.732881015230484
3	6.730327788505523	9.221147105034683
4	3.4110385603858187	6.231234561855358
5	1.9483440468130893	4.449007445958646
6	1.2081051580401385	3.310422829528477
7	0.7993312972748878	2.5553614408363727
8	0.5687610291155907	2.0780083949919486
9	0.40119777664573403	1.6490298093570155
>10	1.8823285294127075	14.862769271131688
>50	0.11113247092059847	3.478694312458013
>100	0.05982113850094523	5.217837089742618
>500	0.004228168292653052	1.3020585650248095
>1k	6.263953026152669E-4	0.42939468441222434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2268	0.16169382119999914	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1510	0.10765329365608409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	7.129357195767158E-5	0.0	0.0	0.0
8	0.0	7.129357195767158E-5	0.0	0.0	0.0
9	0.0	7.129357195767158E-5	0.0	7.129357195767158E-5	0.0
10	0.0	7.129357195767158E-5	0.0	1.4258714391534317E-4	0.0
11	0.0	7.129357195767158E-5	0.0	1.4258714391534317E-4	0.0
12	0.0	7.129357195767158E-5	0.0	1.4258714391534317E-4	0.0
13	0.0	7.129357195767158E-5	0.0	1.4258714391534317E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	35	0.0021696468	16.286173	13
TATGCCG	45	6.756634E-4	14.779249	5
GGTATCA	625	0.0	14.58512	1
CGTTATT	70	7.2726034E-6	13.569388	2
AGTCGGT	50	0.0014978903	13.3018	6
TCGCGTA	80	1.9959643E-6	13.064268	9
GTACCGC	60	4.0869074E-4	12.668382	6
GCGTAAC	85	3.9349925E-6	12.296222	11
TAGGACG	55	0.0030675363	12.09125	4
GTTTAGA	95	1.0454551E-6	11.994342	1
CACCGTG	120	9.988071E-9	11.876608	7
GCGTTAT	80	2.8797522E-5	11.8694	1
TGCACCG	65	8.015849E-4	11.693473	5
GTATCAA	1445	0.0	11.631192	1
CGGTCGG	75	2.0702442E-4	11.40195	10
CGATAAC	75	2.0702442E-4	11.40195	10
CGCGTAA	85	5.312489E-5	11.178383	10
ACGGTAT	145	1.2132659E-9	11.139439	9
CCGACCT	60	0.0058692503	11.084834	9
CGGCGTC	60	0.005877056	11.082854	14
>>END_MODULE