FastQCFastQC Report
Thu 2 Feb 2017
SRR4062001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402651
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16200.027.0332971
GGTATCA6900.026.1532551
GTCCTAC2050.019.3232251
ATACCGT3200.019.2492686
TACCGTC2850.018.525617
TCAACGC23200.018.3010274
CGTCGTA2900.018.20620310
ACCGTCG2900.018.2062038
ATCAACG23400.018.144613
CAACGCA23550.018.0290375
TATCAAC23700.018.0083942
AACGCAG24300.017.5631166
GGCGTTA3550.017.35330842
TATACCG902.2163348E-417.1104585
CCGTCGT3000.016.8660269
TATATAC2350.016.8504243
CTTACAC2251.8189894E-1216.621593
TCTAGCG2400.016.50054728
CCTAGCG800.001988597216.4999582
GCGAGTA800.001988597216.49995830
Produced by FastQC (version 0.11.5)