FastQCFastQC Report
Thu 2 Feb 2017
SRR4062000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337774
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC20710.1548094072690903No Hit
GTATCAACGCAGAGTACTTTTTTTT20030.14972633643649824No Hit
GAATAGGACCGCGGTTCTATTTTGT16530.12356347185698033No Hit
GGGTAGGCACACGCTGAGCCAGTCA16280.1216946958155862No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15410.11519135519153459No Hit
TCGTAGTTCCGACCATAAACGATGC15260.1140700895666981No Hit
GTATCTGATCGTCTTCGAACCTCCG15060.1125750687335828No Hit
GATTAAGAGGGACGGCCGGGGGCAT14960.11182755831702514No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14920.11152855415040208No Hit
ATCAGATACCGTCGTAGTTCCGACC14560.10883751665079454No Hit
GAACTACGACGGTATCTGATCGTCT14440.10794050415092533No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13580.10151191456852951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1300.014.6163649
GGTATCA5250.013.9285311
ACGTTCG707.252891E-613.5728467
GCGTAAC1351.8189894E-1213.37176711
ACTATAC1102.744855E-912.9544433
CGCGTAA1403.6379788E-1212.89372110
GCGTTAT1700.012.848511
GTATCAA16300.012.7011421
GATATAC1155.347829E-912.3870331
CGTTATT1700.012.292642
AACCGCG951.0380882E-612.0012527
CGGTTCT3900.011.44991512
AAGACGG4250.011.4011915
CAAGACG4100.011.3548434
GGTCGCG1850.011.2984777
TATACGC1604.5474735E-1111.2824297
GGGTCGC1950.011.20636
GGTTCTA4100.011.1222813
ACAGCGC1201.2732198E-711.0840758
TTAGAGT1553.110472E-1011.0334094
Produced by FastQC (version 0.11.5)