##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062000_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337774
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3103917403089	32.0	32.0	32.0	32.0	32.0
2	30.946517124716134	32.0	32.0	32.0	32.0	32.0
3	30.96460837181766	32.0	32.0	32.0	32.0	32.0
4	30.970002406983543	32.0	32.0	32.0	32.0	32.0
5	30.922410661292567	32.0	32.0	32.0	32.0	32.0
6	34.550708116617606	36.0	36.0	36.0	32.0	36.0
7	34.49367082930301	36.0	36.0	36.0	32.0	36.0
8	34.47574104445145	36.0	36.0	36.0	32.0	36.0
9	34.581404631873546	36.0	36.0	36.0	32.0	36.0
10	34.35069899699052	36.0	36.0	36.0	32.0	36.0
11	34.53120706487045	36.0	36.0	36.0	32.0	36.0
12	34.41105747308589	36.0	36.0	36.0	32.0	36.0
13	34.4819416433568	36.0	36.0	36.0	32.0	36.0
14	34.406343672399075	36.0	36.0	36.0	32.0	36.0
15	34.369293318602395	36.0	36.0	36.0	32.0	36.0
16	34.371529869768736	36.0	36.0	36.0	32.0	36.0
17	34.31031026167349	36.0	36.0	36.0	32.0	36.0
18	34.31576783522478	36.0	36.0	36.0	32.0	36.0
19	34.30648525087197	36.0	36.0	36.0	32.0	36.0
20	34.30261837948712	36.0	36.0	36.0	32.0	36.0
21	34.27684721036588	36.0	36.0	36.0	32.0	36.0
22	34.251894565150764	36.0	36.0	36.0	32.0	36.0
23	34.230719837580935	36.0	36.0	36.0	32.0	36.0
24	34.20409650658482	36.0	36.0	36.0	32.0	36.0
25	33.867390904592256	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	22.0
5	88.0
6	267.0
7	57.0
8	199.0
9	206.0
10	107.0
11	29.0
12	51.0
13	65.0
14	154.0
15	278.0
16	435.0
17	582.0
18	786.0
19	1033.0
20	1563.0
21	2126.0
22	3129.0
23	4455.0
24	6312.0
25	9177.0
26	12481.0
27	16880.0
28	22799.0
29	30860.0
30	41646.0
31	57759.0
32	83800.0
33	121718.0
34	278085.0
35	640625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.338917801276466	17.295430115162834	11.64889314808807	25.716758935472633
2	16.861867137101296	19.25517839389432	37.36247635991239	26.520478109091993
3	19.408655039304392	22.66797432133022	28.06802993652094	29.855340702844448
4	12.87950827270471	15.337995233438892	35.13634338377994	36.64615311007646
5	15.112069900211988	36.16139561142122	33.03502862035316	15.691505868013625
6	35.131437820404464	34.7407070551436	16.351005473521706	13.77684965093023
7	31.14418717416665	29.87658459675796	19.876217934855404	19.103010294219985
8	28.43063890925962	32.962195249711904	18.781672328898335	19.825493512130148
9	26.802567543194638	14.509672441418658	18.626138380011064	40.06162163537564
10	16.588623805966687	26.342459457881557	30.397868890987993	26.671047845163763
11	37.45377731068534	21.203376838802207	21.790311424901418	19.552534425611036
12	24.771560305983442	23.57210221403871	28.113127048996272	23.543210430981574
13	29.29243863774828	19.27157165503315	25.149728834008688	26.286260873209876
14	23.61410871602063	19.761691656962906	24.59378919545116	32.0304104315653
15	25.22526231326694	27.343154705321414	21.732143311591784	25.699439669819863
16	25.95453895725333	25.80725793113564	23.381012572022144	24.857190539588885
17	24.13167015552813	26.223939252647703	24.755099887213852	24.88929070461031
18	24.884682964209066	25.01040173132702	25.842689902687688	24.262225401776227
19	25.656379583059607	24.921024947287613	25.01747103250435	24.40512443714843
20	25.949150209043488	24.358728800117316	24.464298005931628	25.227822984907565
21	26.931217683354298	24.159664337087445	24.12801637613639	24.78110160342187
22	26.128803677240303	24.139409489560677	24.870607443523337	24.861179389675684
23	24.37462314302087	24.381580502635565	25.43303952827606	25.810756826067504
24	24.90427017134854	24.879954271143983	25.11929754731126	25.096478010196215
25	24.770707408513022	24.630427252207262	25.050519560290912	25.5483457789888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	160.0
2	256.0
3	352.0
4	352.0
5	352.0
6	794.0
7	1236.0
8	1236.0
9	1236.0
10	1500.5
11	1765.0
12	1765.0
13	1765.0
14	2505.0
15	3245.0
16	3245.0
17	3245.0
18	5529.5
19	7814.0
20	7814.0
21	7814.0
22	12603.0
23	17392.0
24	17392.0
25	17392.0
26	26099.5
27	34807.0
28	34807.0
29	34807.0
30	44016.0
31	53225.0
32	53225.0
33	53225.0
34	66946.5
35	80668.0
36	80668.0
37	80668.0
38	94098.5
39	107529.0
40	107529.0
41	107529.0
42	123774.5
43	140020.0
44	140020.0
45	140020.0
46	155451.5
47	170883.0
48	170883.0
49	170883.0
50	176695.5
51	182508.0
52	182508.0
53	182508.0
54	170538.0
55	158568.0
56	158568.0
57	158568.0
58	146876.0
59	135184.0
60	135184.0
61	135184.0
62	119334.0
63	103484.0
64	103484.0
65	103484.0
66	84988.5
67	66493.0
68	66493.0
69	66493.0
70	50712.5
71	34932.0
72	34932.0
73	34932.0
74	26442.0
75	17952.0
76	17952.0
77	17952.0
78	14749.5
79	11547.0
80	11547.0
81	11547.0
82	8015.0
83	4483.0
84	4483.0
85	4483.0
86	3296.0
87	2109.0
88	2109.0
89	2109.0
90	1420.0
91	731.0
92	731.0
93	731.0
94	448.0
95	165.0
96	165.0
97	165.0
98	343.5
99	522.0
100	522.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011960166664922475
2	2.990041666230619E-4
3	7.475104165576547E-4
4	0.009119627082003388
5	0.03281570728688104
6	0.059202824991366254
7	0.1041282010264813
8	0.10719299373436769
9	0.11623786977471531
10	0.13126282914752416
11	0.13828942706316613
12	0.13111332706421264
13	0.12647876248155518
14	0.11377108540007505
15	0.1327578499806395
16	0.11608836769140378
17	0.12087243435737276
18	0.10898701873410606
19	0.09538232915275674
20	0.09044876040347623
21	0.08932749477863974
22	0.09994214269375844
23	0.0792361041551114
24	0.08970124998691856
25	0.08693546144565525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1337774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.24799462114949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33794268461727	41.71338350702418
2	12.901153351427649	13.739210885268886
3	3.84638107186203	6.144362358762018
4	1.5691250412449727	3.3421104702449314
5	0.8377446080511451	2.2304110191702184
6	0.5167172886613804	1.6508495644377679
7	0.3431768043453652	1.2791433642319694
8	0.2607208239940344	1.1106288826924806
9	0.1956448909066826	0.9375928288788974
>10	1.0148619925478677	9.70554238015991
>50	0.08430162183711637	3.1170026355954312
>100	0.0788325396931529	8.902217654543978
>500	0.009871680597905904	3.568844086797475
>1k	0.0035256002135378227	2.5587003621920035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2071	0.1548094072690903	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2003	0.14972633643649824	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1653	0.12356347185698033	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1628	0.1216946958155862	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1541	0.11519135519153459	No Hit
TCGTAGTTCCGACCATAAACGATGC	1526	0.1140700895666981	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1506	0.1125750687335828	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1496	0.11182755831702514	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1492	0.11152855415040208	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1456	0.10883751665079454	No Hit
GAACTACGACGGTATCTGATCGTCT	1444	0.10794050415092533	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1358	0.10151191456852951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.475104165576547E-5	0.0	0.0	0.0	0.0
6	7.475104165576547E-5	0.0	0.0	0.0	0.0
7	7.475104165576547E-5	0.0	0.0	7.475104165576547E-5	0.0
8	7.475104165576547E-5	0.0	0.0	7.475104165576547E-5	0.0
9	7.475104165576547E-5	0.0	0.0	2.242531249672964E-4	0.0
10	7.475104165576547E-5	0.0	0.0	2.242531249672964E-4	0.0
11	7.475104165576547E-5	0.0	0.0	2.242531249672964E-4	0.0
12	7.475104165576547E-5	0.0	0.0	2.242531249672964E-4	1.4950208331153094E-4
13	7.475104165576547E-5	7.475104165576547E-5	0.0	2.242531249672964E-4	1.4950208331153094E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	130	0.0	14.616364	9
GGTATCA	525	0.0	13.928531	1
ACGTTCG	70	7.252891E-6	13.572846	7
GCGTAAC	135	1.8189894E-12	13.371767	11
ACTATAC	110	2.744855E-9	12.954443	3
CGCGTAA	140	3.6379788E-12	12.893721	10
GCGTTAT	170	0.0	12.84851	1
GTATCAA	1630	0.0	12.701142	1
GATATAC	115	5.347829E-9	12.387033	1
CGTTATT	170	0.0	12.29264	2
AACCGCG	95	1.0380882E-6	12.001252	7
CGGTTCT	390	0.0	11.449915	12
AAGACGG	425	0.0	11.401191	5
CAAGACG	410	0.0	11.354843	4
GGTCGCG	185	0.0	11.298477	7
TATACGC	160	4.5474735E-11	11.282429	7
GGGTCGC	195	0.0	11.2063	6
GGTTCTA	410	0.0	11.12228	13
ACAGCGC	120	1.2732198E-7	11.084075	8
TTAGAGT	155	3.110472E-10	11.033409	4
>>END_MODULE