FastQCFastQC Report
Thu 2 Feb 2017
SRR4062000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337774
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19590.14643729060364455No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17410.1301415635226877No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16300.12184419789889771No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16020.11975116873253629No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16010.11967641769088053No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15630.11683587810796144No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG15450.11549035935815766No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15230.11384583644173081No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14220.10629598123449849No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13730.102633180193366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG602.9468902E-729.3320645
GGTATCA6750.029.0191671
GTATCAA17250.027.686581
TCGCGTC904.0856503E-721.99822417
CTCGGTA550.00448595119.99838624
TAGGACG2600.019.4606954
ATACCGT4550.019.339826
GTAAACG4000.019.24988627
TAAACGC4150.018.554828
GTATAGG1557.185008E-1018.4590931
TCAACGC25700.018.4038724
ATCAACG25850.018.3821813
AACGCAG26300.018.0676576
TACCGTC4750.018.0616997
CAACGCA26500.017.93135
CGTCGTA4350.017.69972210
CGCGGGA4250.017.6018744
CCGTGCG750.001291666917.598589
ACCGTCG4550.017.405198
GTCCTAC3700.017.2502351
Produced by FastQC (version 0.11.5)