##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337774
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30237469109132	32.0	32.0	32.0	32.0	32.0
2	31.433110525395172	32.0	32.0	32.0	32.0	32.0
3	31.5629560747929	32.0	32.0	32.0	32.0	32.0
4	31.642023241593872	32.0	32.0	32.0	32.0	32.0
5	31.61500148754573	32.0	32.0	32.0	32.0	32.0
6	35.25901235933723	36.0	36.0	36.0	36.0	36.0
7	35.26212349769094	36.0	36.0	36.0	36.0	36.0
8	35.211910232969096	36.0	36.0	36.0	36.0	36.0
9	35.30460899972641	36.0	36.0	36.0	36.0	36.0
10	35.16830346530879	36.0	36.0	36.0	36.0	36.0
11	35.29826413131067	36.0	36.0	36.0	36.0	36.0
12	35.213846284947984	36.0	36.0	36.0	36.0	36.0
13	35.25526359459819	36.0	36.0	36.0	36.0	36.0
14	35.22092371357195	36.0	36.0	36.0	36.0	36.0
15	35.20011526610623	36.0	36.0	36.0	36.0	36.0
16	35.207226332698944	36.0	36.0	36.0	36.0	36.0
17	35.18634238668116	36.0	36.0	36.0	36.0	36.0
18	35.19070485747219	36.0	36.0	36.0	36.0	36.0
19	35.18034660562995	36.0	36.0	36.0	36.0	36.0
20	35.16840363170461	36.0	36.0	36.0	36.0	36.0
21	35.16642945669448	36.0	36.0	36.0	36.0	36.0
22	35.1571393972375	36.0	36.0	36.0	36.0	36.0
23	35.11965698242005	36.0	36.0	36.0	36.0	36.0
24	35.10396374873484	36.0	36.0	36.0	36.0	36.0
25	35.08362174776906	36.0	36.0	36.0	36.0	36.0
26	35.05010338069061	36.0	36.0	36.0	36.0	36.0
27	35.03723274633832	36.0	36.0	36.0	36.0	36.0
28	35.009749030852745	36.0	36.0	36.0	36.0	36.0
29	34.98800694287675	36.0	36.0	36.0	36.0	36.0
30	34.96747058920266	36.0	36.0	36.0	36.0	36.0
31	34.975116873253626	36.0	36.0	36.0	36.0	36.0
32	34.954164903787934	36.0	36.0	36.0	36.0	36.0
33	34.92200775317804	36.0	36.0	36.0	32.0	36.0
34	34.91815284195985	36.0	36.0	36.0	32.0	36.0
35	34.909673831304836	36.0	36.0	36.0	32.0	36.0
36	34.88372176466279	36.0	36.0	36.0	32.0	36.0
37	34.86770859651929	36.0	36.0	36.0	32.0	36.0
38	34.855043527531556	36.0	36.0	36.0	32.0	36.0
39	34.83273931172231	36.0	36.0	36.0	32.0	36.0
40	34.829441295764454	36.0	36.0	36.0	32.0	36.0
41	34.80316929466412	36.0	36.0	36.0	32.0	36.0
42	34.76719759839853	36.0	36.0	36.0	32.0	36.0
43	34.766186964315345	36.0	36.0	36.0	32.0	36.0
44	34.71571057592688	36.0	36.0	36.0	32.0	36.0
45	34.70818314603214	36.0	36.0	36.0	32.0	36.0
46	34.6827192036921	36.0	36.0	36.0	32.0	36.0
47	34.67105729368339	36.0	36.0	36.0	32.0	36.0
48	34.64387407738527	36.0	36.0	36.0	32.0	36.0
49	34.628198783950054	36.0	36.0	36.0	32.0	36.0
50	34.21587951328102	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	5.0
21	23.0
22	78.0
23	236.0
24	577.0
25	1319.0
26	2786.0
27	5400.0
28	9605.0
29	15796.0
30	24579.0
31	37402.0
32	57323.0
33	99016.0
34	227745.0
35	855877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.118845640494555	17.788935705660013	12.065815988903553	26.026402664941884
2	16.3465800909165	19.74073622778014	36.97166278815081	26.941020893152555
3	18.716427237648436	23.19329389444791	28.274750591658034	29.815528276245622
4	12.584188360664806	15.797287135196228	35.21147817194832	36.40704633219064
5	14.782691246802523	36.57852522174896	32.8475512306264	15.791232300822111
6	35.239338242989085	34.96844006303017	16.198574944011387	13.593646749969352
7	31.185760823577073	29.925682514385837	19.836459671065516	19.05209699097157
8	28.587265113539356	32.94689536498691	18.726855208727333	19.7389843127464
9	27.053448489804705	14.273860906251729	18.569504266041946	40.10318633790162
10	16.632268703285234	26.41950465437709	30.316055115479234	26.632171526858446
11	37.72692547470649	21.027169013600204	21.788209368697554	19.457696142995754
12	24.826764460962764	23.448205750747135	28.128517970897924	23.596511817392177
13	29.313620985308432	19.15271189304023	25.212330333823203	26.321336787828137
14	23.686213067379093	19.623419202346586	24.574629197457867	32.11573853281646
15	25.332156253597393	27.305509002267947	21.66838344892336	25.6939512952113
16	26.088812310084357	25.739498342384497	23.320463609079322	24.85122573845182
17	24.286015425624957	26.191419477430415	24.639587852656728	24.8829772442879
18	25.073461860754737	24.88164997095911	25.85797697509809	24.186911193188063
19	25.813926717068803	24.83035251096224	24.89613342761931	24.459587344349643
20	26.041098245587808	24.260672611883958	24.473788468869838	25.2244406736584
21	27.044104609597735	24.09001819440354	24.14496021002053	24.7209169859782
22	26.27671041595965	24.088149418362146	24.874231372414172	24.760908793264033
23	24.608303956130044	24.253609733198182	25.34744336105106	25.79064294962071
24	24.90667165015885	24.81435245748458	25.16456737058494	25.11440852177163
25	25.064865458834824	24.35360663911333	25.121601711506248	25.459926190545602
26	24.63534327778048	25.534679110307117	25.431521978822886	24.39845563308952
27	25.380634011278485	24.718934822779435	24.833977191859873	25.066453974082204
28	24.855410527952472	24.514764683764493	25.412688291485797	25.217136496797245
29	24.76817746561626	24.740519476830457	25.308928522039302	25.18237453551398
30	24.677969142447076	24.987067934457603	25.549724913287886	24.78523800980744
31	25.417934948361747	24.616076928137016	24.535644446570707	25.430343676930526
32	25.29401695218062	24.646069595287056	24.375543351101804	25.68437010143052
33	24.663227617839496	24.60551977054403	25.055670879887693	25.675581731728776
34	25.58649930331717	24.640445877013054	25.12954413620299	24.64351068346679
35	25.96718576320497	24.368035533681724	25.284782993826305	24.379995709287
36	24.47681667728133	25.172525662070967	24.865074168094413	25.48558349255329
37	25.745335648658607	24.787311260000912	24.798225083404784	24.669128007935694
38	24.89658193386925	24.518416414132734	25.21135857028168	25.373643081716345
39	25.87461045159583	24.394382579328088	24.37322792384623	25.35777904522985
40	25.768555132795623	24.67666340252809	25.011474319202854	24.543307145473435
41	24.784041334892162	25.0988969587877	25.398204156811854	24.71885754950828
42	26.044339920954524	25.13188573615676	24.78300619491056	24.04076814797815
43	25.46811411758022	24.33161796146842	24.958361615539417	25.241906305411945
44	24.797939173935273	24.958287110268547	24.731333108073763	25.512440607722414
45	25.07680830292148	25.19035751181465	24.70282101385465	25.03001317140922
46	24.96049301133705	24.800670983406317	24.81711665512978	25.421719350126853
47	25.18846874053919	24.647039256067067	25.218818094494132	24.945673908899614
48	25.96646218847975	25.274909285542353	24.061047428673092	24.697581097304806
49	25.04696413233532	25.399727746327155	24.27393414032651	25.279373981011016
50	25.230348778624474	25.542663299829414	24.317026897321927	24.909961024224184
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	39.5
2	38.0
3	94.0
4	150.0
5	158.0
6	166.0
7	130.0
8	94.0
9	126.5
10	159.0
11	278.5
12	398.0
13	729.5
14	1061.0
15	1422.0
16	1783.0
17	1836.0
18	1889.0
19	1930.5
20	1972.0
21	2464.5
22	2957.0
23	3585.0
24	4213.0
25	5685.5
26	7158.0
27	9360.5
28	11563.0
29	13445.5
30	15328.0
31	17527.5
32	19727.0
33	22963.0
34	26199.0
35	30823.0
36	35447.0
37	42256.0
38	49065.0
39	53701.5
40	58338.0
41	63619.0
42	68900.0
43	70650.0
44	72400.0
45	79880.0
46	87360.0
47	93190.0
48	99020.0
49	103155.0
50	107290.0
51	103203.0
52	99116.0
53	98496.0
54	97876.0
55	98897.5
56	99919.0
57	96893.0
58	93867.0
59	85986.0
60	78105.0
61	67968.0
62	57831.0
63	50231.5
64	42632.0
65	35897.5
66	29163.0
67	25187.5
68	21212.0
69	19120.5
70	17029.0
71	13357.5
72	9686.0
73	8594.5
74	7503.0
75	5485.5
76	3468.0
77	3193.0
78	2918.0
79	2456.5
80	1995.0
81	1569.5
82	1144.0
83	976.0
84	808.0
85	633.5
86	459.0
87	314.0
88	169.0
89	118.0
90	67.0
91	47.0
92	27.0
93	24.0
94	21.0
95	18.5
96	16.0
97	17.0
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030573176037208077
2	0.004709315624313225
3	5.980083332461238E-4
4	0.0
5	0.0
6	1.4950208331153094E-4
7	0.0
8	0.0
9	0.0
10	7.475104165576547E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.727593749018893E-4
17	0.0
18	3.737552082788274E-4
19	0.0
20	2.990041666230619E-4
21	0.0
22	0.0
23	1.4950208331153094E-4
24	0.0017940249997383713
25	3.737552082788274E-4
26	6.727593749018893E-4
27	4.485062499345928E-4
28	0.0015697718747710748
29	3.737552082788274E-4
30	0.0010465145831807167
31	4.485062499345928E-4
32	8.222614582134202E-4
33	7.475104165576547E-5
34	4.485062499345928E-4
35	7.475104165576547E-5
36	1.4950208331153094E-4
37	0.0015697718747710748
38	0.0
39	5.232572915903584E-4
40	2.990041666230619E-4
41	0.0013455187498037785
42	0.004709315624313225
43	0.0049335687492805215
44	0.0043355604160343975
45	0.0020930291663614335
46	0.002691037499607557
47	0.001420269791459544
48	0.004485062499345928
49	0.0038123031244440392
50	0.0014950208331153095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1337774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.196026401907936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26930382846153	43.201545605747164
2	13.285895123217067	14.666572359881384
3	3.7708268167515415	6.244039696033217
4	1.5311590779429436	3.380555875666389
5	0.7963644117988311	2.1978075549594083
6	0.5094706059528474	1.687245181030164
7	0.33371543458365205	1.289383615660241
8	0.2278298512826048	1.006024198922993
9	0.17449178987843209	0.8668128096941499
>10	0.9476854996469988	9.3506254248595
>50	0.07238702434167749	2.771127392411757
>100	0.06941503906430482	7.978331043458711
>500	0.008591622808129513	3.217794219912921
>1k	0.002863874269376505	2.142135021761998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1959	0.14643729060364455	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1741	0.1301415635226877	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1630	0.12184419789889771	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1602	0.11975116873253629	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1601	0.11967641769088053	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1563	0.11683587810796144	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1545	0.11549035935815766	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1523	0.11384583644173081	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1422	0.10629598123449849	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1373	0.102633180193366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	2.990041666230619E-4
16	0.0	0.0	0.0	0.0	3.737552082788274E-4
17	0.0	0.0	0.0	0.0	4.485062499345928E-4
18	0.0	0.0	0.0	0.0	4.485062499345928E-4
19	0.0	7.475104165576547E-5	0.0	0.0	4.485062499345928E-4
20	0.0	7.475104165576547E-5	0.0	0.0	5.232572915903583E-4
21	0.0	7.475104165576547E-5	0.0	0.0	5.232572915903583E-4
22	0.0	7.475104165576547E-5	0.0	0.0	5.232572915903583E-4
23	0.0	7.475104165576547E-5	0.0	7.475104165576547E-5	5.232572915903583E-4
24	0.0	7.475104165576547E-5	0.0	7.475104165576547E-5	5.232572915903583E-4
25	0.0	7.475104165576547E-5	0.0	1.4950208331153094E-4	5.232572915903583E-4
26	0.0	7.475104165576547E-5	0.0	2.242531249672964E-4	5.232572915903583E-4
27	0.0	7.475104165576547E-5	0.0	2.990041666230619E-4	5.980083332461238E-4
28	0.0	7.475104165576547E-5	0.0	3.737552082788274E-4	6.727593749018893E-4
29	0.0	7.475104165576547E-5	0.0	4.485062499345928E-4	6.727593749018893E-4
30	0.0	7.475104165576547E-5	0.0	7.475104165576547E-4	6.727593749018893E-4
31	0.0	7.475104165576547E-5	0.0	0.0020182781247056678	6.727593749018893E-4
32	0.0	7.475104165576547E-5	0.0	0.0035132989578209772	6.727593749018893E-4
33	0.0	7.475104165576547E-5	0.0	0.0043355604160343975	6.727593749018893E-4
34	0.0	7.475104165576547E-5	0.0	0.006578091665707362	8.222614582134202E-4
35	0.0	7.475104165576547E-5	0.0	0.009343880206970684	8.222614582134202E-4
36	0.0	7.475104165576547E-5	0.0	0.012259170831545537	8.222614582134202E-4
37	0.0	7.475104165576547E-5	0.0	0.01779074791407218	8.222614582134202E-4
38	0.0	7.475104165576547E-5	0.0	0.02661137082945251	8.970124998691856E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	60	2.9468902E-7	29.332064	5
GGTATCA	675	0.0	29.019167	1
GTATCAA	1725	0.0	27.68658	1
TCGCGTC	90	4.0856503E-7	21.998224	17
CTCGGTA	55	0.004485951	19.998386	24
TAGGACG	260	0.0	19.460695	4
ATACCGT	455	0.0	19.33982	6
GTAAACG	400	0.0	19.249886	27
TAAACGC	415	0.0	18.5548	28
GTATAGG	155	7.185008E-10	18.459093	1
TCAACGC	2570	0.0	18.403872	4
ATCAACG	2585	0.0	18.382181	3
AACGCAG	2630	0.0	18.067657	6
TACCGTC	475	0.0	18.061699	7
CAACGCA	2650	0.0	17.9313	5
CGTCGTA	435	0.0	17.699722	10
CGCGGGA	425	0.0	17.60187	44
CCGTGCG	75	0.0012916669	17.59858	9
ACCGTCG	455	0.0	17.40519	8
GTCCTAC	370	0.0	17.250235	1
>>END_MODULE