FastQCFastQC Report
Thu 2 Feb 2017
SRR4061999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308378
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27600.8950054802871801No Hit
TATCAACGCAGAGTACTTTTTTTTT17580.5700795776611821No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14060.4559339511897736No Hit
GGTATCAACGCAGAGTACTTTTTTT10400.3372484418473432No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10110.3278443987573692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9890.32071029710290616No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5410.17543404523020448No Hit
ATCAACGCAGAGTACTTTTTTTTTT3490.11317279442761806No Hit
GTCCTAAAGTGTGTATTTCTCATTT3230.10474158338143447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA307.701312E-419.000814
GAGTAGG402.768303E-416.6122231
TTATACA551.12200305E-515.5461194
GTGTTAA400.00529333614.2390481
GTATAAT400.00529333614.2390481
TAGAAAT604.0740223E-412.6672084
TAGCCCT604.0740223E-412.6672084
GAACAGT853.9125243E-612.2946426
AATGTCC951.0302683E-612.0005128
ATGTCCA657.988543E-411.6928079
AGAAATG907.402732E-611.6116075
GACGTGG752.0627184E-411.4004887
TAGAACA1104.915564E-711.2277534
GATTAAA855.332192E-511.167881
TGTAGAA951.3520914E-510.9986842
AGACTGG700.001484997710.8576076
TGGCGAG1252.2077438E-710.64218318
AGGACGT1451.4206307E-810.4832075
AAAGTGT1201.5075238E-610.2921066
TAGGACG1502.406341E-810.1337674
Produced by FastQC (version 0.11.5)