FastQCFastQC Report
Thu 2 Feb 2017
SRR4061999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308378
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.31098197666500205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.18905369384327028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.17251554909883327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG250.002346431135.2111631
TAGAACG250.00235016935.199744
GTGCGGC300.00573867129.33311811
ATCCCGT407.02048E-427.49979620
TAGACTG450.001395915524.4442655
ATACATG1105.1968527E-922.0069751
GCTATTC500.002576230821.999843
CCCGTTT500.002576230821.9998422
ATTATGC500.002576230821.999843
GTCCTAC1950.021.4426961
GTCCTAA1351.10958354E-1021.1919021
GTATAAT755.4424047E-520.5398431
TAATACT755.4560194E-520.5331824
GTATTAT550.004467965620.006341
GGGAACG550.004476363319.9998537
TCCCGTT550.004476363319.99985321
CACACCG550.004476363319.9998535
CACCGTC550.004476363319.9998537
TAAGCCA550.004476363319.9998534
TTTCTCG1001.1179654E-619.79985641
Produced by FastQC (version 0.11.5)