##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308378
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070196317506436	32.0	32.0	32.0	32.0	32.0
2	31.410389197672984	32.0	32.0	32.0	32.0	32.0
3	31.568818138777733	32.0	32.0	32.0	32.0	32.0
4	31.67307979168423	32.0	32.0	32.0	32.0	32.0
5	31.658805102828346	32.0	32.0	32.0	32.0	32.0
6	35.292021480131524	36.0	36.0	36.0	36.0	36.0
7	35.29848108490229	36.0	36.0	36.0	36.0	36.0
8	35.26153940942609	36.0	36.0	36.0	36.0	36.0
9	35.28356108412403	36.0	36.0	36.0	36.0	36.0
10	35.2178916783947	36.0	36.0	36.0	36.0	36.0
11	35.32537340536614	36.0	36.0	36.0	36.0	36.0
12	35.26104650785724	36.0	36.0	36.0	36.0	36.0
13	35.27074240056035	36.0	36.0	36.0	36.0	36.0
14	35.24107102322474	36.0	36.0	36.0	36.0	36.0
15	35.21909150458204	36.0	36.0	36.0	36.0	36.0
16	35.23453683466395	36.0	36.0	36.0	36.0	36.0
17	35.223089844282015	36.0	36.0	36.0	36.0	36.0
18	35.20055581137435	36.0	36.0	36.0	36.0	36.0
19	35.184789446717986	36.0	36.0	36.0	36.0	36.0
20	35.15230009922887	36.0	36.0	36.0	36.0	36.0
21	35.12710050652122	36.0	36.0	36.0	36.0	36.0
22	35.12344914358352	36.0	36.0	36.0	36.0	36.0
23	35.104641057403576	36.0	36.0	36.0	36.0	36.0
24	35.101923613227925	36.0	36.0	36.0	36.0	36.0
25	35.0904279812438	36.0	36.0	36.0	36.0	36.0
26	35.03107225547867	36.0	36.0	36.0	36.0	36.0
27	35.02880879959012	36.0	36.0	36.0	36.0	36.0
28	34.99793435329369	36.0	36.0	36.0	36.0	36.0
29	34.95593719396325	36.0	36.0	36.0	36.0	36.0
30	34.937210177120285	36.0	36.0	36.0	36.0	36.0
31	34.917581669250076	36.0	36.0	36.0	36.0	36.0
32	34.876080654261976	36.0	36.0	36.0	32.0	36.0
33	34.831576831032045	36.0	36.0	36.0	32.0	36.0
34	34.796214386240266	36.0	36.0	36.0	32.0	36.0
35	34.764217940319995	36.0	36.0	36.0	32.0	36.0
36	34.70754074544877	36.0	36.0	36.0	32.0	36.0
37	34.675566998942855	36.0	36.0	36.0	32.0	36.0
38	34.64556485871236	36.0	36.0	36.0	32.0	36.0
39	34.5935605004248	36.0	36.0	36.0	32.0	36.0
40	34.57260894097504	36.0	36.0	36.0	32.0	36.0
41	34.5346230924385	36.0	36.0	36.0	32.0	36.0
42	34.497927867746725	36.0	36.0	36.0	32.0	36.0
43	34.44550519168034	36.0	36.0	36.0	32.0	36.0
44	34.390543423979665	36.0	36.0	36.0	32.0	36.0
45	34.344609537645354	36.0	36.0	36.0	32.0	36.0
46	34.228485819351576	36.0	36.0	36.0	32.0	36.0
47	34.154946202387976	36.0	36.0	36.0	32.0	36.0
48	34.12585852427865	36.0	36.0	36.0	32.0	36.0
49	34.038903553431176	36.0	36.0	36.0	32.0	36.0
50	33.649997730058566	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	18.0
20	38.0
21	49.0
22	88.0
23	172.0
24	306.0
25	571.0
26	1118.0
27	1935.0
28	3335.0
29	4891.0
30	6799.0
31	9636.0
32	13862.0
33	22227.0
34	46322.0
35	197005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09976774059609	18.362117063929727	12.247790940585709	25.290324254888475
2	14.79611360820864	20.915299748993053	39.483652118641324	24.804934524156984
3	18.48802443754094	25.95775314711166	28.89959724753387	26.654625167813528
4	11.840663082321047	16.396111266043622	36.71532988734605	35.04789576428928
5	13.068701398932477	38.295533403809614	33.62593959361563	15.009825603642282
6	32.283536061379415	36.56984794585848	17.57005224125016	13.576563751511946
7	28.318816517390992	31.714000350219536	21.17206804635869	18.79511508603078
8	27.24772843717775	34.01215391500042	19.66774542931078	19.07237221851105
9	27.72247047454747	14.004241547710928	19.30909468250005	38.96419329524156
10	15.370746291888526	28.19040268761066	32.728988449240866	23.709862571259947
11	35.153934457062434	21.985031357619544	23.638521554715318	19.2225126306027
12	24.685288833833802	24.49720797203432	30.245996796139803	20.571506397992074
13	29.91523390125106	21.188606191103126	25.781021992489737	23.115137915156076
14	22.734436308686092	20.385695477628104	27.815862350751352	29.06400586293445
15	23.81460415464138	28.411235561551084	24.786139089040073	22.988021194767462
16	22.81979525061856	26.86160122188101	26.865168284275416	23.453435243225012
17	21.793383444992834	26.602416514796772	27.70106816958408	23.90313187062631
18	21.985426928726852	25.55313787993268	30.108600835989712	22.352834355350755
19	24.128180350089824	24.614920649332962	29.062708753542733	22.194190247034484
20	24.68188185851039	24.889744986639688	28.742833424131582	21.685539730718347
21	24.549740902399	24.362308595295385	28.180025812476895	22.907924689828715
22	24.203736972157547	25.190837219256885	28.226397473230907	22.37902833535466
23	23.036088943079413	25.355652334642336	28.820891311608843	22.787367410669408
24	23.521361978111067	24.91803810295906	29.068828536684233	22.49177138224564
25	23.146343598906533	25.080339973474025	29.250884469334615	22.522431958284827
26	22.392217267936765	25.61102553708958	29.546493717065264	22.45026347790839
27	22.706872734112032	25.76091679691807	28.91224406410315	22.619966404866755
28	22.388795387404215	25.821326769853393	28.815428069253795	22.9744497734886
29	22.738327636861257	25.64579833840287	28.651849353715246	22.96402467102063
30	22.63373284561704	25.549653669546267	28.98150310011155	22.83511038472514
31	23.14310081491162	25.211024168469116	29.00475716412054	22.641117852498727
32	22.395784353465746	26.005674908796106	28.99002837454398	22.608512363194162
33	22.319037025987587	25.878953751564637	29.433357762226876	22.3686514602209
34	22.5336766394054	25.713257278499484	29.44087374421968	22.312192337875437
35	22.525277419271152	25.894843341613218	29.10940469164467	22.47047454747096
36	22.28149310746262	26.029178570386247	29.14354831910292	22.545780003048215
37	22.45567042403331	26.085377401320482	28.45329666766114	23.00565550698507
38	22.798967500924192	26.55150497117174	28.442366186952377	22.20716134095169
39	23.105085317370243	25.966832912853704	28.387887592500118	22.54019417727594
40	23.401474813378385	26.04465947635694	28.68719558463963	21.866670125625046
41	23.023267126064045	25.80624239967572	28.882042967166598	22.288447507093636
42	22.538623185585866	26.796253778002622	28.484518296558615	22.180604739852903
43	22.259479898690827	26.685756721785687	28.71323732094966	22.341526058573823
44	22.18017194132851	26.83932144467037	28.35313155684409	22.627375057157035
45	22.21580848731702	27.01075994110894	28.034543367297303	22.738888204276737
46	22.644978645990406	26.47054054667562	27.8260418389418	23.058438968392174
47	23.095182504475055	26.701170000259424	27.564077619529403	22.639569875736115
48	22.61710186662516	27.1766483765939	27.11470859114553	23.09154116563542
49	22.94862939289872	27.148495137287714	26.994457902434437	22.90841756737913
50	22.623900276613064	27.783236534975504	26.778933304796464	22.81392988361497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	145.0
2	204.0
3	388.5
4	573.0
5	550.5
6	528.0
7	437.5
8	347.0
9	454.5
10	562.0
11	774.0
12	986.0
13	1731.0
14	2476.0
15	2912.0
16	3348.0
17	3173.5
18	2999.0
19	2726.5
20	2454.0
21	2489.0
22	2524.0
23	2622.0
24	2720.0
25	3057.5
26	3395.0
27	3970.5
28	4546.0
29	5271.0
30	5996.0
31	7176.5
32	8357.0
33	9761.5
34	11166.0
35	12563.0
36	13960.0
37	14461.0
38	14962.0
39	15244.0
40	15526.0
41	15990.0
42	16454.0
43	16803.0
44	17152.0
45	17690.0
46	18228.0
47	18709.5
48	19191.0
49	19653.0
50	20115.0
51	19969.5
52	19824.0
53	19352.0
54	18880.0
55	18346.0
56	17812.0
57	16822.5
58	15833.0
59	14598.0
60	13363.0
61	11850.5
62	10338.0
63	8920.5
64	7503.0
65	6291.5
66	5080.0
67	4336.0
68	3592.0
69	3017.5
70	2443.0
71	2022.5
72	1602.0
73	1381.0
74	1160.0
75	951.0
76	742.0
77	636.5
78	531.0
79	440.5
80	350.0
81	275.5
82	201.0
83	171.0
84	141.0
85	109.5
86	78.0
87	52.5
88	27.0
89	19.5
90	12.0
91	7.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03307628948887405
2	0.006485546958602754
3	0.0
4	0.0
5	0.0
6	3.242773479301377E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.242773479301377E-4
17	0.0
18	3.242773479301377E-4
19	0.0
20	6.485546958602754E-4
21	0.0
22	0.0
23	3.242773479301377E-4
24	9.728320437904131E-4
25	3.242773479301377E-4
26	9.728320437904131E-4
27	0.0
28	0.0016213867396506884
29	0.0
30	6.485546958602754E-4
31	3.242773479301377E-4
32	9.728320437904131E-4
33	0.0
34	0.0012971093917205508
35	0.0
36	3.242773479301377E-4
37	0.0019456640875808262
38	0.0
39	0.0
40	0.0
41	9.728320437904131E-4
42	0.004539882871021928
43	0.004864160218952065
44	0.005512714914812341
45	0.0038913281751616524
46	0.0016213867396506884
47	6.485546958602754E-4
48	0.004539882871021928
49	0.0035670508272315146
50	0.0016213867396506884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	308378.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.55863210802222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.16668106084319	62.8396065792147
2	11.819335714540093	17.861056780322862
3	2.881136156197168	6.530841206376692
4	0.9477000195434475	2.864276685017953
5	0.42172074584531183	1.5932321343823326
6	0.21433203446378654	0.9716781204607913
7	0.13048556725840865	0.6901517680319282
8	0.08111134251698641	0.4902929671223007
9	0.06120280053220948	0.41619599004544944
>10	0.24868809209396947	3.3285565468901277
>50	0.019842147556452642	1.0876048532831666
>100	0.006470265507530164	0.6505421897365974
>500	0.001294053101506033	0.6759641791150817
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.31098197666500205	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.18905369384327028	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.17251554909883327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.485546958602754E-4
7	0.0	0.0	0.0	0.0	6.485546958602754E-4
8	0.0	0.0	0.0	0.0	6.485546958602754E-4
9	0.0	0.0	0.0	0.0	6.485546958602754E-4
10	0.0	0.0	0.0	0.0	6.485546958602754E-4
11	0.0	0.0	0.0	0.0	6.485546958602754E-4
12	0.0	0.0	0.0	0.0	0.0012971093917205508
13	0.0	0.0	0.0	0.0	0.0012971093917205508
14	0.0	0.0	0.0	0.0	0.0012971093917205508
15	0.0	0.0	0.0	0.0	0.0016213867396506884
16	0.0	0.0	0.0	0.0	0.0016213867396506884
17	0.0	0.0	0.0	0.0	0.0016213867396506884
18	0.0	0.0	0.0	0.0	0.0016213867396506884
19	0.0	0.0	0.0	0.0	0.0016213867396506884
20	0.0	0.0	0.0	0.0	0.0016213867396506884
21	0.0	0.0	0.0	3.242773479301377E-4	0.0016213867396506884
22	0.0	0.0	0.0	6.485546958602754E-4	0.0019456640875808262
23	0.0	0.0	0.0	0.0025942187834411016	0.0019456640875808262
24	0.0	0.0	0.0	0.00421560552309179	0.0019456640875808262
25	0.0	0.0	0.0	0.006161269610672616	0.0019456640875808262
26	0.0	0.0	0.0	0.008106933698253441	0.0025942187834411016
27	0.0	0.0	0.0	0.00843121104618358	0.0025942187834411016
28	0.0	0.0	0.0	0.01264681656927537	0.0025942187834411016
29	0.0	0.0	0.0	0.0171866994402973	0.0025942187834411016
30	0.0	0.0	0.0	0.02950923866164253	0.0025942187834411016
31	0.0	0.0	0.0	0.04766877014573024	0.0025942187834411016
32	0.0	0.0	0.0	0.06388263754223712	0.0025942187834411016
33	0.0	0.0	0.0	0.0843121104618358	0.0025942187834411016
34	0.0	0.0	0.0	0.10182308725006324	0.002918496131371239
35	0.0	0.0	0.0	0.12517105630103315	0.002918496131371239
36	0.0	0.0	0.0	0.14819474800407292	0.002918496131371239
37	0.0	0.0	0.0	0.18613519771189904	0.002918496131371239
38	0.0	0.0	0.0	0.23283113581383885	0.002918496131371239
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAG	25	0.0023464311	35.211163	1
TAGAACG	25	0.002350169	35.19974	4
GTGCGGC	30	0.005738671	29.333118	11
ATCCCGT	40	7.02048E-4	27.499796	20
TAGACTG	45	0.0013959155	24.444265	5
ATACATG	110	5.1968527E-9	22.006975	1
GCTATTC	50	0.0025762308	21.99984	3
CCCGTTT	50	0.0025762308	21.99984	22
ATTATGC	50	0.0025762308	21.99984	3
GTCCTAC	195	0.0	21.442696	1
GTCCTAA	135	1.10958354E-10	21.191902	1
GTATAAT	75	5.4424047E-5	20.539843	1
TAATACT	75	5.4560194E-5	20.533182	4
GTATTAT	55	0.0044679656	20.00634	1
GGGAACG	55	0.0044763633	19.999853	7
TCCCGTT	55	0.0044763633	19.999853	21
CACACCG	55	0.0044763633	19.999853	5
CACCGTC	55	0.0044763633	19.999853	7
TAAGCCA	55	0.0044763633	19.999853	4
TTTCTCG	100	1.1179654E-6	19.799856	41
>>END_MODULE