FastQCFastQC Report
Thu 2 Feb 2017
SRR4061998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427867
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30050.21045377475633234No Hit
GCGCAAGACGGACCAGAGCGAAAGC23960.16780274353283606No Hit
GGGTAGGCACACGCTGAGCCAGTCA20200.14146975873803372No Hit
GAATAGGACCGCGGTTCTATTTTGT18910.1324353038483276No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18680.13082450956566682No Hit
GTCTTGCGCCGGTCCAAGAATTTCA18300.1281631972725751No Hit
TATCAACGCAGAGTACTTTTTTTTT17850.12501164324128228No Hit
TCGTAGTTCCGACCATAAACGATGC17790.1245914360371099No Hit
GATTAAGAGGGACGGCCGGGGGCAT17510.12263046908430547No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC17430.12207019281207562No Hit
GAACTACGACGGTATCTGATCGTCT17350.1215099165398458No Hit
ATCAGATACCGTCGTAGTTCCGACC17160.12017926039329994No Hit
GTATCTGATCGTCTTCGAACCTCCG15920.11149497817373748No Hit
GAATAACGCCGCCGCATCGCCAGTC15540.10883366588064573No Hit
ACCATACTCCCCCCGGAACCCAAAG14690.10288073048820374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7850.013.9113671
TTAGAAC707.264027E-613.5709773
TCCAACG707.279834E-613.56812318
GACCGTC500.001497043813.3028247
TCTATAC604.0928047E-412.6662453
TAGGACG2600.012.4235354
CGTTATT1405.2750693E-1112.211312
TAGACTC550.003064966312.09262855
ACGCCCC2050.012.04584517
AGGCCCG3900.011.93759410
TGTAGGA3350.011.9075462
GACGTGA1603.6379788E-1211.8775227
TTAGGAC2000.011.8746053
CCTATTC3050.011.8356723
CTATTCC3150.011.762344
TACCACG658.015505E-411.6935613
AGAATTT3900.011.68945917
CGGACCA4150.011.67758759
TCTAGAT2050.011.5825432
GTCCTAT3050.011.5197631
Produced by FastQC (version 0.11.5)