##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061998_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1427867
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266623572083393	32.0	32.0	32.0	32.0	32.0
2	30.82948131723753	32.0	32.0	32.0	32.0	32.0
3	30.848407449713452	32.0	32.0	32.0	32.0	32.0
4	30.861522116555673	32.0	32.0	32.0	32.0	32.0
5	30.789270989524933	32.0	32.0	32.0	32.0	32.0
6	34.43738317364292	36.0	36.0	36.0	32.0	36.0
7	34.37348786686715	36.0	36.0	36.0	32.0	36.0
8	34.334150169448556	36.0	36.0	36.0	32.0	36.0
9	34.45971508550866	36.0	36.0	36.0	32.0	36.0
10	34.16653581881226	36.0	36.0	36.0	32.0	36.0
11	34.43419870338064	36.0	36.0	36.0	32.0	36.0
12	34.2646941206709	36.0	36.0	36.0	32.0	36.0
13	34.360569997065554	36.0	36.0	36.0	32.0	36.0
14	34.26885697337357	36.0	36.0	36.0	32.0	36.0
15	34.21320403090764	36.0	36.0	36.0	32.0	36.0
16	34.22681804397749	36.0	36.0	36.0	32.0	36.0
17	34.15054413331214	36.0	36.0	36.0	32.0	36.0
18	34.158835521795794	36.0	36.0	36.0	32.0	36.0
19	34.159245223819866	36.0	36.0	36.0	32.0	36.0
20	34.14134859899416	36.0	36.0	36.0	32.0	36.0
21	34.11487834651267	36.0	36.0	36.0	32.0	36.0
22	34.09515592138484	36.0	36.0	36.0	32.0	36.0
23	34.060460112881664	36.0	36.0	36.0	32.0	36.0
24	34.03294494515246	36.0	36.0	36.0	32.0	36.0
25	33.696813498736226	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	21.0
5	114.0
6	292.0
7	59.0
8	234.0
9	202.0
10	112.0
11	32.0
12	56.0
13	66.0
14	244.0
15	350.0
16	534.0
17	693.0
18	997.0
19	1357.0
20	1950.0
21	2843.0
22	4055.0
23	6093.0
24	8449.0
25	11832.0
26	15848.0
27	20825.0
28	27163.0
29	36080.0
30	47508.0
31	64407.0
32	91473.0
33	131198.0
34	292060.0
35	660717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58295175837305	17.19895731636428	11.609380230751883	25.608710694510783
2	16.933381703885377	18.96998737976813	37.3878921411393	26.7087387752072
3	19.47018429742516	22.510969251079416	27.69013660350666	30.328709847988765
4	12.730036211835738	15.032254449433008	35.012572581266504	37.22513675746475
5	15.209188341667987	36.04584362612742	32.89537290156609	15.849595130638502
6	35.44069612699819	34.77446441122902	16.04569367076366	13.73914579100913
7	31.464238666422688	29.76620892776643	19.460879063405496	19.308673342405385
8	28.65465095327137	32.781415397472756	18.577521263073173	19.9864123861827
9	26.998362044303338	14.32559137492918	18.384930807590692	40.29111577317679
10	16.66067061774732	26.009387498343195	30.05970101105307	27.270240872856412
11	37.812231741596676	21.154612221081393	21.515937003809622	19.517219033512312
12	24.90680067546719	23.332370250270692	27.964285513921695	23.796543560340428
13	29.316886914508306	18.969948353663455	25.1617585700311	26.551406161797136
14	23.73799876175057	19.582360765302653	24.241221645946208	32.43841882700057
15	25.379106637942645	27.237051748126206	21.523593532606256	25.860248081324894
16	26.13583775309009	25.71877725828301	23.19474789857044	24.95063709005646
17	24.014374868522545	26.103148446812984	24.61328097608863	25.269195708575836
18	25.09594903658333	24.88995833911762	25.722054185789865	24.292038438509184
19	25.871172714705132	24.656003499552742	24.878581222992736	24.59424256274939
20	25.951717181205325	24.189006647492242	24.468421432642177	25.390854738660252
21	27.05332194916098	23.837363358386924	24.118602887261645	24.990711805190454
22	26.21634070369303	24.007966971141816	24.74675561855847	25.02893670660668
23	24.62515104702574	24.082292942914737	25.240413595493962	26.05214241456556
24	24.87179055806507	24.79888852437872	25.156178587551135	25.173142330005078
25	25.009270247517364	24.256785505748958	25.18009514883917	25.553849097894517
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	567.0
1	567.0
2	510.0
3	453.0
4	453.0
5	453.0
6	1030.0
7	1607.0
8	1607.0
9	1607.0
10	1884.0
11	2161.0
12	2161.0
13	2161.0
14	2661.5
15	3162.0
16	3162.0
17	3162.0
18	5619.5
19	8077.0
20	8077.0
21	8077.0
22	12887.0
23	17697.0
24	17697.0
25	17697.0
26	27074.0
27	36451.0
28	36451.0
29	36451.0
30	46373.5
31	56296.0
32	56296.0
33	56296.0
34	69555.5
35	82815.0
36	82815.0
37	82815.0
38	95984.0
39	109153.0
40	109153.0
41	109153.0
42	126223.5
43	143294.0
44	143294.0
45	143294.0
46	161986.0
47	180678.0
48	180678.0
49	180678.0
50	188388.0
51	196098.0
52	196098.0
53	196098.0
54	182901.5
55	169705.0
56	169705.0
57	169705.0
58	157891.0
59	146077.0
60	146077.0
61	146077.0
62	130632.0
63	115187.0
64	115187.0
65	115187.0
66	95321.5
67	75456.0
68	75456.0
69	75456.0
70	57409.0
71	39362.0
72	39362.0
73	39362.0
74	30217.5
75	21073.0
76	21073.0
77	21073.0
78	17163.0
79	13253.0
80	13253.0
81	13253.0
82	9199.5
83	5146.0
84	5146.0
85	5146.0
86	3822.5
87	2499.0
88	2499.0
89	2499.0
90	1653.0
91	807.0
92	807.0
93	807.0
94	482.5
95	158.0
96	158.0
97	158.0
98	396.5
99	635.0
100	635.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.104489423734845E-4
2	7.003453402872958E-5
3	8.404144083447548E-4
4	0.010995421842510542
5	0.03452702527616368
6	0.06205059714945439
7	0.1056120773153242
8	0.10981414935704796
9	0.11898867331481154
10	0.1350265816073906
11	0.14027917165954532
12	0.1324353038483276
13	0.12760292100034526
14	0.11625732648769109
15	0.13187502757609776
16	0.11870853517869662
17	0.12375102162876514
18	0.11128487457165129
19	0.09797831310619266
20	0.09202537771375065
21	0.09279575758806669
22	0.1035110412944623
23	0.08117002493929758
24	0.09069472156720479
25	0.08824351287619925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1427867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.391546643508015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.87697185848414	30.045844584895352
2	15.100050261712502	12.80228969977402
3	5.572599410625142	7.08693323523303
4	2.6463869076503626	4.487377361297171
5	1.4649435746699233	3.1050611937863715
6	0.8903587548294216	2.264621081088429
7	0.6175278132487192	1.832457136929761
8	0.44286787310626646	1.5019083279756384
9	0.3070518780765892	1.171476361031442
>10	1.8083116431849025	14.22361758915221
>50	0.1441581166923358	4.277386377971923
>100	0.10888437329141151	9.599335649748173
>500	0.01441846246059472	4.165786745309243
>1k	0.00546907196781179	3.4359046558073003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3005	0.21045377475633234	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2396	0.16780274353283606	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2020	0.14146975873803372	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1891	0.1324353038483276	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1868	0.13082450956566682	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1830	0.1281631972725751	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1785	0.12501164324128228	No Hit
TCGTAGTTCCGACCATAAACGATGC	1779	0.1245914360371099	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1751	0.12263046908430547	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1743	0.12207019281207562	No Hit
GAACTACGACGGTATCTGATCGTCT	1735	0.1215099165398458	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1716	0.12017926039329994	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1592	0.11149497817373748	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1554	0.10883366588064573	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1469	0.10288073048820374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.003453402872956E-5
2	0.0	0.0	0.0	0.0	7.003453402872956E-5
3	0.0	0.0	0.0	0.0	7.003453402872956E-5
4	0.0	0.0	0.0	0.0	7.003453402872956E-5
5	0.0	0.0	0.0	0.0	7.003453402872956E-5
6	0.0	0.0	0.0	0.0	7.003453402872956E-5
7	0.0	0.0	0.0	0.0	7.003453402872956E-5
8	0.0	0.0	0.0	0.0	7.003453402872956E-5
9	0.0	0.0	0.0	2.101036020861887E-4	7.003453402872956E-5
10	0.0	0.0	0.0	2.101036020861887E-4	7.003453402872956E-5
11	0.0	0.0	0.0	2.8013813611491825E-4	7.003453402872956E-5
12	0.0	0.0	0.0	2.8013813611491825E-4	7.703798743160253E-4
13	0.0	0.0	0.0	2.8013813611491825E-4	9.104489423734844E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	785	0.0	13.911367	1
TTAGAAC	70	7.264027E-6	13.570977	3
TCCAACG	70	7.279834E-6	13.568123	18
GACCGTC	50	0.0014970438	13.302824	7
TCTATAC	60	4.0928047E-4	12.666245	3
TAGGACG	260	0.0	12.423535	4
CGTTATT	140	5.2750693E-11	12.21131	2
TAGACTC	55	0.0030649663	12.0926285	5
ACGCCCC	205	0.0	12.045845	17
AGGCCCG	390	0.0	11.937594	10
TGTAGGA	335	0.0	11.907546	2
GACGTGA	160	3.6379788E-12	11.877522	7
TTAGGAC	200	0.0	11.874605	3
CCTATTC	305	0.0	11.835672	3
CTATTCC	315	0.0	11.76234	4
TACCACG	65	8.015505E-4	11.69356	13
AGAATTT	390	0.0	11.689459	17
CGGACCA	415	0.0	11.6775875	9
TCTAGAT	205	0.0	11.582543	2
GTCCTAT	305	0.0	11.519763	1
>>END_MODULE