FastQCFastQC Report
Thu 2 Feb 2017
SRR4061998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427867
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23500.1645811549675145No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19920.1395087917852293No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19230.13467640893724694No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19220.1346063744032182No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18470.1293537843510635No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18270.1279530936704889No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC17710.12403115976488006No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17690.1238910906968226No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA16470.1153468775453176No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15140.10603228451949658No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC15060.10547200824726673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG300.005746809329.3318165
GTATCAA19850.028.4951271
GGTATCA8650.023.6627251
CGCAATA4450.022.24837736
TATGCCG654.9344864E-420.3066435
AACGCAG28950.019.6811926
ATACGAA5050.019.60569240
TCAACGC29000.019.4196854
TAGGACG3300.019.3323334
CGTCTAT808.962204E-519.257771
CAACGCA29800.019.0459965
TACGAAT5200.019.04014241
GCGCAAT5200.019.0388135
ATCAACG29700.019.0360533
GTCCTAC4000.018.7075481
CTAGCGG5350.018.50436429
TAACGAT600.007414954218.3323864
CGAATGC5450.018.16801643
GCAATAC5500.018.0009637
CAATACG5500.018.0009638
Produced by FastQC (version 0.11.5)