##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1427867
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28484445680165	32.0	32.0	32.0	32.0	32.0
2	31.466671615773738	32.0	32.0	32.0	32.0	32.0
3	31.5867024029549	32.0	32.0	32.0	32.0	32.0
4	31.663682261723256	32.0	32.0	32.0	32.0	32.0
5	31.63317171697364	32.0	32.0	32.0	32.0	32.0
6	35.29153485583741	36.0	36.0	36.0	36.0	36.0
7	35.29110904587052	36.0	36.0	36.0	36.0	36.0
8	35.23890040178812	36.0	36.0	36.0	36.0	36.0
9	35.320140461261445	36.0	36.0	36.0	36.0	36.0
10	35.21021285595927	36.0	36.0	36.0	36.0	36.0
11	35.3247116152975	36.0	36.0	36.0	36.0	36.0
12	35.240399140816336	36.0	36.0	36.0	36.0	36.0
13	35.28593909656852	36.0	36.0	36.0	36.0	36.0
14	35.23871130854624	36.0	36.0	36.0	36.0	36.0
15	35.218371879173624	36.0	36.0	36.0	36.0	36.0
16	35.22928956268336	36.0	36.0	36.0	36.0	36.0
17	35.21273970194703	36.0	36.0	36.0	36.0	36.0
18	35.20930310736224	36.0	36.0	36.0	36.0	36.0
19	35.194315717080094	36.0	36.0	36.0	36.0	36.0
20	35.193536232716355	36.0	36.0	36.0	36.0	36.0
21	35.180915309339035	36.0	36.0	36.0	36.0	36.0
22	35.16999692548396	36.0	36.0	36.0	36.0	36.0
23	35.13810600006863	36.0	36.0	36.0	36.0	36.0
24	35.12223197258568	36.0	36.0	36.0	36.0	36.0
25	35.09824234329948	36.0	36.0	36.0	36.0	36.0
26	35.065227363613	36.0	36.0	36.0	36.0	36.0
27	35.05274160688636	36.0	36.0	36.0	36.0	36.0
28	35.02761041469549	36.0	36.0	36.0	36.0	36.0
29	35.00713721936287	36.0	36.0	36.0	36.0	36.0
30	34.982070458943305	36.0	36.0	36.0	36.0	36.0
31	34.989797369082694	36.0	36.0	36.0	36.0	36.0
32	34.95690775121212	36.0	36.0	36.0	36.0	36.0
33	34.94442619655752	36.0	36.0	36.0	36.0	36.0
34	34.92579420912452	36.0	36.0	36.0	36.0	36.0
35	34.91478968279259	36.0	36.0	36.0	36.0	36.0
36	34.8906109602645	36.0	36.0	36.0	32.0	36.0
37	34.87197126903276	36.0	36.0	36.0	32.0	36.0
38	34.85329866157002	36.0	36.0	36.0	32.0	36.0
39	34.835641554850696	36.0	36.0	36.0	32.0	36.0
40	34.82295409866605	36.0	36.0	36.0	32.0	36.0
41	34.79326786038195	36.0	36.0	36.0	32.0	36.0
42	34.76463774287101	36.0	36.0	36.0	32.0	36.0
43	34.77058787688209	36.0	36.0	36.0	32.0	36.0
44	34.7080533411025	36.0	36.0	36.0	32.0	36.0
45	34.696761673181044	36.0	36.0	36.0	32.0	36.0
46	34.66529165531524	36.0	36.0	36.0	32.0	36.0
47	34.64366289017114	36.0	36.0	36.0	32.0	36.0
48	34.61443607843027	36.0	36.0	36.0	32.0	36.0
49	34.608083245848526	36.0	36.0	36.0	32.0	36.0
50	34.19116696443016	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	11.0
21	36.0
22	104.0
23	249.0
24	654.0
25	1565.0
26	3289.0
27	6260.0
28	10987.0
29	17349.0
30	26081.0
31	38969.0
32	59052.0
33	101103.0
34	233861.0
35	928290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37971883006292	17.59319262459603	12.018343931909433	26.008744613431624
2	16.37881162794281	19.383009689018504	37.050025143542314	27.188153539496373
3	18.89756776044727	22.850123366350086	28.037533065193323	30.214775808009325
4	12.481221964261326	15.381846323006727	35.23995615832029	36.896975554411654
5	14.865740296540226	36.33139501087987	32.7807141701573	16.022150522422606
6	35.68668208361289	34.782745719836456	15.899531188500882	13.631041008049774
7	31.48997770800782	29.742616083991013	19.51253162934643	19.254874578654736
8	28.71983174903545	32.87372003134746	18.479382183354613	19.92706603626248
9	27.12059316448941	14.017411985850222	18.34652667230211	40.51546817735826
10	16.54831997658045	25.989185267255284	30.19321827593186	27.269276480232403
11	37.967261656722926	20.945438195574237	21.582192178963446	19.505107968739384
12	24.96731138124209	23.1422114244534	28.017105234591178	23.873371959713335
13	29.435724755877125	18.815337843090425	25.21971584188163	26.529221559150816
14	23.87813430802729	19.307190375574194	24.275720357708387	32.538954958690134
15	25.470369439170454	27.14216380097026	21.537650215321175	25.84981654453811
16	26.355234047202337	25.6057171151372	23.164359356320293	24.874689481340166
17	24.20218409697822	25.993527408365065	24.588984828418894	25.21530366623782
18	25.30844959649603	24.593677142198818	25.865924487364722	24.231948773940427
19	26.044862721808126	24.483722923773712	24.88971311753826	24.581701236879905
20	26.128206977840314	23.959021278652084	24.499829465431908	25.412942278075697
21	27.246604373239506	23.718262077814025	24.179930049458424	24.855203499488045
22	26.311204054719383	23.861466088928452	24.8454512920321	24.981878564320066
23	24.557049080901795	23.950970223417166	25.380865304681738	26.111115390999302
24	25.080312664363447	24.605054987999498	25.173039036822313	25.141593310814734
25	25.28043656849432	23.977790586351773	25.21096211611338	25.53081072904053
26	24.603584252484666	25.214359701286615	25.628686906321853	24.553369139906867
27	25.36538869625447	24.44268112557017	24.994134591343848	25.19779558683151
28	25.01698371944156	24.209329069544307	25.456599418141273	25.31708779287286
29	24.64984287664258	24.411654091895628	25.47156266830268	25.466940363159107
30	24.65292149649087	24.786129046039246	25.649526875078354	24.91142258239153
31	25.429260370217595	24.394707335367606	24.553546103512733	25.622486190902066
32	25.416935425696796	24.379928270228362	24.463129762812716	25.74000654126213
33	24.85828462414435	24.211653507131643	25.10893909915664	25.821122769567367
34	25.61954820342402	24.414243743237233	25.315455701034068	24.650752352304682
35	26.092629334887167	24.166254045381105	25.40313741584452	24.33797920388721
36	24.633280130208753	24.85283931699234	24.91362954797421	25.6002510048247
37	25.95910471886945	24.510134874867106	24.776270763852022	24.754489642411414
38	25.173282945820585	24.08039404230226	25.187569990762444	25.55875302111471
39	25.892664546944378	24.074419060692225	24.456249212107632	25.576667180255768
40	26.03980491139212	24.49925202960506	24.92079077559207	24.540152283410745
41	24.632542191026936	24.85889634142498	25.668224485608093	24.84033698193999
42	26.315262345084555	24.969550950941837	24.76097759132088	23.954209112652727
43	25.568407450597245	23.802830218412176	25.236079156467444	25.392683174523135
44	24.731336426186154	24.74394316322643	24.833941258208387	25.690779152379033
45	25.14756596695979	24.94243036315095	24.65395185441465	25.256051815474606
46	24.91229741209956	24.731604517882644	24.739728694521855	25.61636937549594
47	25.417410207780637	24.53342503284654	25.070035269761853	24.97912948961097
48	26.18753628811697	25.092361793489186	23.700438924253874	25.019662994139974
49	25.197068779192616	25.23369853361918	24.30660855399317	25.262624133195033
50	25.413177125136045	25.222259107825955	24.16100425958687	25.203559507451125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	164.5
2	63.0
3	156.0
4	249.0
5	265.5
6	282.0
7	224.5
8	167.0
9	221.0
10	275.0
11	479.0
12	683.0
13	1258.5
14	1834.0
15	2408.5
16	2983.0
17	2888.0
18	2793.0
19	2666.0
20	2539.0
21	2905.0
22	3271.0
23	3894.0
24	4517.0
25	5947.0
26	7377.0
27	9690.0
28	12003.0
29	14192.0
30	16381.0
31	18287.0
32	20193.0
33	23352.0
34	26511.0
35	31482.0
36	36453.0
37	43176.0
38	49899.0
39	54734.0
40	59569.0
41	64482.0
42	69395.0
43	71220.5
44	73046.0
45	81000.5
46	88955.0
47	95292.5
48	101630.0
49	106517.0
50	111404.0
51	108309.5
52	105215.0
53	105254.5
54	105294.0
55	107461.5
56	109629.0
57	106043.5
58	102458.0
59	94662.5
60	86867.0
61	76744.5
62	66622.0
63	57492.0
64	48362.0
65	40714.5
66	33067.0
67	29005.5
68	24944.0
69	22636.5
70	20329.0
71	15703.0
72	11077.0
73	9958.0
74	8839.0
75	6336.0
76	3833.0
77	3448.5
78	3064.0
79	2671.5
80	2279.0
81	1820.0
82	1361.0
83	1172.5
84	984.0
85	771.0
86	558.0
87	373.0
88	188.0
89	135.5
90	83.0
91	56.0
92	29.0
93	22.5
94	16.0
95	13.5
96	11.0
97	11.5
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03319636912961781
2	0.004552244711867422
3	4.202072041723774E-4
4	1.4006906805745915E-4
5	0.0
6	7.003453402872958E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.80483476402214E-4
17	0.0
18	0.0
19	0.0
20	2.801381361149183E-4
21	7.003453402872958E-5
22	0.0
23	0.0
24	7.003453402872956E-4
25	2.801381361149183E-4
26	7.003453402872956E-4
27	2.801381361149183E-4
28	0.0020310014868331577
29	5.602762722298366E-4
30	0.0014006906805745913
31	2.801381361149183E-4
32	5.602762722298366E-4
33	3.501726701436478E-4
34	8.404144083447548E-4
35	2.801381361149183E-4
36	4.202072041723774E-4
37	0.001750863350718239
38	0.0
39	4.90241738201107E-4
40	2.101036020861887E-4
41	0.0011205525444596732
42	0.005042486450068529
43	0.004342141109781233
44	0.004132037507695045
45	0.0021710705548906163
46	0.002101036020861887
47	0.0010505180104309435
48	0.004062002973666315
49	0.0046222792458961515
50	0.0020310014868331577
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1427867.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.66209214115734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2348281425132	30.66599537876761
2	15.587517972338194	13.611672919203524
3	5.716914254852902	7.488373108754496
4	2.722089067445947	4.754084147170479
5	1.5076320266252279	3.2913184230735233
6	0.8897182605096313	2.3308176402025054
7	0.6000364811153576	1.833919368856019
8	0.42532652542083765	1.4856516754402331
9	0.32244263897416464	1.2670668191815115
>10	1.7404016799456485	13.826035640311673
>50	0.1364739055447719	4.113247198391334
>100	0.09871490865178507	8.616246486119525
>500	0.013549075939013808	3.972396002635664
>1k	0.004355060123254439	2.743175191891885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2350	0.1645811549675145	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1992	0.1395087917852293	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1923	0.13467640893724694	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1922	0.1346063744032182	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1847	0.1293537843510635	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1827	0.1279530936704889	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1771	0.12403115976488006	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1769	0.1238910906968226	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1647	0.1153468775453176	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1514	0.10603228451949658	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1506	0.10547200824726673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8013813611491825E-4
2	0.0	0.0	0.0	0.0	2.8013813611491825E-4
3	0.0	0.0	0.0	0.0	2.8013813611491825E-4
4	0.0	0.0	0.0	7.003453402872956E-5	2.8013813611491825E-4
5	0.0	0.0	0.0	7.003453402872956E-5	2.8013813611491825E-4
6	0.0	0.0	0.0	7.003453402872956E-5	4.90241738201107E-4
7	0.0	0.0	0.0	7.003453402872956E-5	4.90241738201107E-4
8	0.0	0.0	0.0	7.003453402872956E-5	4.90241738201107E-4
9	0.0	0.0	0.0	1.4006906805745913E-4	4.90241738201107E-4
10	0.0	0.0	0.0	1.4006906805745913E-4	4.90241738201107E-4
11	0.0	0.0	0.0	1.4006906805745913E-4	4.90241738201107E-4
12	0.0	0.0	0.0	1.4006906805745913E-4	0.0011905870784884027
13	0.0	0.0	0.0	1.4006906805745913E-4	0.0011905870784884027
14	0.0	0.0	0.0	1.4006906805745913E-4	0.0012606216125171322
15	0.0	0.0	0.0	1.4006906805745913E-4	0.0014006906805745913
16	0.0	0.0	0.0	2.101036020861887E-4	0.0015407597486320505
17	0.0	0.0	0.0	2.8013813611491825E-4	0.0015407597486320505
18	0.0	0.0	0.0	2.8013813611491825E-4	0.0016808288166895095
19	0.0	0.0	0.0	2.8013813611491825E-4	0.0017508633507182392
20	0.0	0.0	0.0	2.8013813611491825E-4	0.0018909324187756983
21	0.0	0.0	0.0	3.501726701436478E-4	0.0020310014868331573
22	0.0	0.0	0.0	4.202072041723774E-4	0.0020310014868331573
23	0.0	0.0	0.0	5.602762722298365E-4	0.0020310014868331573
24	0.0	0.0	0.0	8.404144083447548E-4	0.0020310014868331573
25	0.0	0.0	0.0	0.0013306561465458618	0.0020310014868331573
26	0.0	0.0	0.0	0.0017508633507182392	0.0023811741569768055
27	0.0	0.0	0.0	0.001960966952804428	0.0024512086910055348
28	0.0	0.0	0.0	0.0024512086910055348	0.0024512086910055348
29	0.0	0.0	0.0	0.003361657633379019	0.0024512086910055348
30	0.0	0.0	0.0	0.006303108062585661	0.0024512086910055348
31	0.0	0.0	0.0	0.012606216125171321	0.0024512086910055348
32	0.0	0.0	0.0	0.01778877164329731	0.0024512086910055348
33	0.0	0.0	0.0	0.023741707035739322	0.0024512086910055348
34	0.0	0.0	0.0	0.029554573360123876	0.0025212432250342645
35	0.0	0.0	0.0	0.035997750490767	0.002591277759062994
36	0.0	0.0	0.0	0.04461199817630073	0.002591277759062994
37	0.0	0.0	0.0	0.0570781452334146	0.002591277759062994
38	0.0	0.0	0.0	0.07458677874059699	0.0026613122930917235
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	30	0.0057468093	29.331816	5
GTATCAA	1985	0.0	28.495127	1
GGTATCA	865	0.0	23.662725	1
CGCAATA	445	0.0	22.248377	36
TATGCCG	65	4.9344864E-4	20.306643	5
AACGCAG	2895	0.0	19.681192	6
ATACGAA	505	0.0	19.605692	40
TCAACGC	2900	0.0	19.419685	4
TAGGACG	330	0.0	19.332333	4
CGTCTAT	80	8.962204E-5	19.25777	1
CAACGCA	2980	0.0	19.045996	5
TACGAAT	520	0.0	19.040142	41
GCGCAAT	520	0.0	19.03881	35
ATCAACG	2970	0.0	19.036053	3
GTCCTAC	400	0.0	18.707548	1
CTAGCGG	535	0.0	18.504364	29
TAACGAT	60	0.0074149542	18.332386	4
CGAATGC	545	0.0	18.168016	43
GCAATAC	550	0.0	18.00096	37
CAATACG	550	0.0	18.00096	38
>>END_MODULE