FastQCFastQC Report
Thu 2 Feb 2017
SRR4061997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286734
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA46020.14001741546471358No Hit
GTATCAACGCAGAGTACTTTTTTTT45350.13797891767328904No Hit
GTCCTACAGTGGACATTTCTAAATT43330.13183299895884484No Hit
GTCCTAAAGTGTGTATTTCTCATTT38290.1164986275129049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTT250.006036384518.99841517
GGTATCA11550.015.12809851
GTATCAA35950.013.3395431
TTAACCG500.001499560513.3007145
AACCGCG1102.744855E-912.9562277
CGTTATT2150.012.8115182
CGGTCCA3150.012.3661210
CGACCAT2700.012.31585110
TAGGACC12600.011.9126124
CGTCGTA2650.011.83118410
GTGTAAG3550.011.7699011
TGTAGGA25150.011.3298552
GTAGGAC25100.011.2779443
ACGGTAT2700.011.26055059
CCGTCGT2800.011.1977129
GCGTTAT2550.011.1719651
CGACGCT1201.2767487E-711.08342315
GTCTTAG3650.010.9270991
GTCCTAA9750.010.90842151
AGGACGT21100.010.8963195
Produced by FastQC (version 0.11.5)