##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061997_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3286734
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298647837032142	32.0	32.0	32.0	32.0	32.0
2	31.01220269118219	32.0	32.0	32.0	32.0	32.0
3	31.01422293376951	32.0	32.0	32.0	32.0	32.0
4	31.02052493447903	32.0	32.0	32.0	32.0	32.0
5	30.99911736088165	32.0	32.0	32.0	32.0	32.0
6	34.5274080591858	36.0	36.0	36.0	32.0	36.0
7	34.50315084822806	36.0	36.0	36.0	32.0	36.0
8	34.48143749996197	36.0	36.0	36.0	32.0	36.0
9	34.58240581683823	36.0	36.0	36.0	32.0	36.0
10	34.40858037188285	36.0	36.0	36.0	32.0	36.0
11	34.521052509877585	36.0	36.0	36.0	32.0	36.0
12	34.44281770292333	36.0	36.0	36.0	32.0	36.0
13	34.47431523208145	36.0	36.0	36.0	32.0	36.0
14	34.43589837206175	36.0	36.0	36.0	32.0	36.0
15	34.384676094871075	36.0	36.0	36.0	32.0	36.0
16	34.39668710641019	36.0	36.0	36.0	32.0	36.0
17	34.342386697554474	36.0	36.0	36.0	32.0	36.0
18	34.34222361773116	36.0	36.0	36.0	32.0	36.0
19	34.32705688990956	36.0	36.0	36.0	32.0	36.0
20	34.30161522045897	36.0	36.0	36.0	32.0	36.0
21	34.28185517903183	36.0	36.0	36.0	32.0	36.0
22	34.24921852513772	36.0	36.0	36.0	32.0	36.0
23	34.230141228344	36.0	36.0	36.0	32.0	36.0
24	34.19875262190369	36.0	36.0	36.0	32.0	36.0
25	33.79241459759141	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	43.0
5	242.0
6	715.0
7	147.0
8	586.0
9	419.0
10	261.0
11	63.0
12	130.0
13	111.0
14	362.0
15	592.0
16	1165.0
17	1616.0
18	2299.0
19	2981.0
20	4017.0
21	5300.0
22	7173.0
23	9922.0
24	13740.0
25	19743.0
26	27792.0
27	37173.0
28	51328.0
29	71408.0
30	98406.0
31	142666.0
32	214972.0
33	327525.0
34	768807.0
35	1475029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.32128834197167	17.566316417202028	12.10227264083634	26.01012259998996
2	16.15853704916776	18.95609218532601	38.88418478322051	26.00118598228572
3	19.39717334871647	22.59337283781136	28.088858301112722	29.920595512359448
4	12.731149867514443	15.568817634880276	35.55043275369128	36.149599743914
5	14.711549670091603	36.230878354667276	33.900016709231075	15.157555266010045
6	34.672997065163116	35.14162719047213	16.585786134417965	13.599589609946783
7	30.71783768176503	29.997036510013885	19.979587554073035	19.30553825414805
8	27.441674456401394	34.51877409830203	18.77065650330915	19.268894941987416
9	26.79318712262119	14.828097118289579	18.686369919721997	39.692345839367235
10	15.973383077074466	27.062020952205124	31.68421620248213	25.28037976823828
11	36.69391755633464	21.175138633429782	22.670956856295607	19.45998695393997
12	24.83838124113458	23.257533475342555	29.093143586918902	22.810941696603965
13	29.562060806457364	20.13678878710916	24.843240634878853	25.457909771554622
14	22.696790276585595	20.155304874074083	25.292342707591498	31.855562141748823
15	24.552377151439725	28.546351063781174	21.937243994095777	24.96402779068333
16	24.818723148118814	26.268085038978633	24.789662802513934	24.123529010388616
17	23.53212834155528	26.722023908528342	25.590600113687668	24.15524763622871
18	23.913334827868532	25.70893967601076	27.007053150220283	23.370672345900427
19	25.332346594914945	25.128451110004583	25.36676062000064	24.17244167507983
20	25.598355982828934	25.034565046850105	25.50260928036486	23.864469689956096
21	25.611714762525427	24.63915924622075	24.81073294921613	24.93839304203769
22	25.443542971275733	25.496902440190645	25.117355806847254	23.942198781686372
23	23.911124455055994	25.496238946204585	25.735272847301793	24.857363751437628
24	24.549176221658968	25.099194173908728	26.061337310134785	24.29029229429752
25	24.269666274540494	25.36001659049999	25.845855130950145	24.52446200400937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	457.5
3	767.0
4	767.0
5	767.0
6	1764.0
7	2761.0
8	2761.0
9	2761.0
10	3477.5
11	4194.0
12	4194.0
13	4194.0
14	6394.0
15	8594.0
16	8594.0
17	8594.0
18	15029.5
19	21465.0
20	21465.0
21	21465.0
22	35605.5
23	49746.0
24	49746.0
25	49746.0
26	75276.0
27	100806.0
28	100806.0
29	100806.0
30	133026.5
31	165247.0
32	165247.0
33	165247.0
34	196013.0
35	226779.0
36	226779.0
37	226779.0
38	257938.0
39	289097.0
40	289097.0
41	289097.0
42	322917.0
43	356737.0
44	356737.0
45	356737.0
46	387919.0
47	419101.0
48	419101.0
49	419101.0
50	425795.0
51	432489.0
52	432489.0
53	432489.0
54	402372.5
55	372256.0
56	372256.0
57	372256.0
58	338470.0
59	304684.0
60	304684.0
61	304684.0
62	265969.5
63	227255.0
64	227255.0
65	227255.0
66	186476.5
67	145698.0
68	145698.0
69	145698.0
70	112244.0
71	78790.0
72	78790.0
73	78790.0
74	60221.5
75	41653.0
76	41653.0
77	41653.0
78	31823.0
79	21993.0
80	21993.0
81	21993.0
82	15810.0
83	9627.0
84	9627.0
85	9627.0
86	6844.0
87	4061.0
88	4061.0
89	4061.0
90	2623.5
91	1186.0
92	1186.0
93	1186.0
94	750.0
95	314.0
96	314.0
97	314.0
98	800.0
99	1286.0
100	1286.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.823348649449575E-4
2	2.1297738119361044E-4
3	5.476561230692839E-4
4	0.010283764977634332
5	0.034228507691830244
6	0.06249364871023941
7	0.10475444620708582
8	0.10852718838822978
9	0.11841542394364742
10	0.13116364147509352
11	0.13743126155021976
12	0.13137661885628713
13	0.12641728840849306
14	0.11476438312318551
15	0.13201555099986795
16	0.11896308006671669
17	0.123405179731612
18	0.11141759570442877
19	0.09702640980377482
20	0.09340579432348343
21	0.09170197527393455
22	0.10171191219003424
23	0.0815703369971528
24	0.0919758033354692
25	0.08905497067909968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3286734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.861535259536154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85509703478206	27.14933931282168
2	16.65564606555356	13.9446183008705
3	7.0649374476583375	8.872473842147006
4	3.695309432912593	6.187653044830682
5	2.1356853658888353	4.470153412371542
6	1.3357586544916475	3.355014480793961
7	0.9040731522210707	2.649212309723165
8	0.6119923333842481	2.0495150914024407
9	0.4355107205400179	1.640803264541276
>10	2.0981500050515347	15.26463793052095
>50	0.12142488887983538	3.4937906078580507
>100	0.07516005098384512	6.125337087880421
>500	0.00789301004261213	2.3441309422626704
>1k	0.0033618376098113415	2.453320371975602
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	4602	0.14001741546471358	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4535	0.13797891767328904	No Hit
GTCCTACAGTGGACATTTCTAAATT	4333	0.13183299895884484	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3829	0.1164986275129049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.0850680341031553E-5
2	0.0	0.0	0.0	0.0	6.0850680341031553E-5
3	0.0	0.0	0.0	0.0	6.0850680341031553E-5
4	0.0	0.0	0.0	0.0	6.0850680341031553E-5
5	0.0	0.0	0.0	0.0	1.2170136068206311E-4
6	0.0	0.0	0.0	0.0	1.8255204102309467E-4
7	0.0	0.0	0.0	3.0425340170515777E-5	2.1297738119361044E-4
8	0.0	0.0	0.0	3.0425340170515777E-5	2.4340272136412621E-4
9	0.0	0.0	0.0	1.2170136068206311E-4	2.4340272136412621E-4
10	0.0	0.0	0.0	1.2170136068206311E-4	2.4340272136412621E-4
11	0.0	0.0	0.0	1.2170136068206311E-4	2.4340272136412621E-4
12	0.0	0.0	0.0	1.2170136068206311E-4	2.73828061534642E-4
13	0.0	0.0	0.0	1.521267008525789E-4	3.042534017051578E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGTT	25	0.0060363845	18.998415	17
GGTATCA	1155	0.0	15.1280985	1
GTATCAA	3595	0.0	13.339543	1
TTAACCG	50	0.0014995605	13.300714	5
AACCGCG	110	2.744855E-9	12.956227	7
CGTTATT	215	0.0	12.811518	2
CGGTCCA	315	0.0	12.36612	10
CGACCAT	270	0.0	12.315851	10
TAGGACC	1260	0.0	11.912612	4
CGTCGTA	265	0.0	11.831184	10
GTGTAAG	355	0.0	11.769901	1
TGTAGGA	2515	0.0	11.329855	2
GTAGGAC	2510	0.0	11.277944	3
ACGGTAT	270	0.0	11.2605505	9
CCGTCGT	280	0.0	11.197712	9
GCGTTAT	255	0.0	11.171965	1
CGACGCT	120	1.2767487E-7	11.083423	15
GTCTTAG	365	0.0	10.927099	1
GTCCTAA	975	0.0	10.9084215	1
AGGACGT	2110	0.0	10.896319	5
>>END_MODULE