FastQCFastQC Report
Thu 2 Feb 2017
SRR4061997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286734
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35300.027.7465461
GGTATCA15000.025.531811
TCCGTTA1004.6306013E-821.99980226
GTAGGAC28100.020.903413
TAGGACG28200.020.8292834
GTCCTAC24900.019.4467491
AGGACGT29800.019.1941665
TGTAGGA30500.019.1875882
GTCCTAA16250.018.8271711
GGACGTG30100.018.710516
TCAACGC51800.018.6443374
CTGTAGG31150.018.441921
TATCAAC53900.018.3272022
ATCAACG53100.018.3121743
ATACCGT6400.018.218316
CAACGCA52950.018.1147635
TTAGGAC18500.018.0752373
TAGGACC16200.017.9254934
AACGCAG54150.017.8352096
TACCGTC5600.017.6778767
Produced by FastQC (version 0.11.5)