##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3286734
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20372929479538	32.0	32.0	32.0	32.0	32.0
2	31.26291662178929	32.0	32.0	32.0	32.0	32.0
3	31.46951502616275	32.0	32.0	32.0	32.0	32.0
4	31.57419918983404	32.0	32.0	32.0	32.0	32.0
5	31.522314857241263	32.0	32.0	32.0	32.0	32.0
6	35.145126438586146	36.0	36.0	36.0	36.0	36.0
7	35.16212872718023	36.0	36.0	36.0	36.0	36.0
8	35.0900900407517	36.0	36.0	36.0	36.0	36.0
9	35.21679058907718	36.0	36.0	36.0	36.0	36.0
10	35.05761068586627	36.0	36.0	36.0	36.0	36.0
11	35.221616656535026	36.0	36.0	36.0	36.0	36.0
12	35.13167387442975	36.0	36.0	36.0	36.0	36.0
13	35.17141514950708	36.0	36.0	36.0	36.0	36.0
14	35.134122201553275	36.0	36.0	36.0	36.0	36.0
15	35.090513257233475	36.0	36.0	36.0	36.0	36.0
16	35.1128223336601	36.0	36.0	36.0	36.0	36.0
17	35.09578961972584	36.0	36.0	36.0	36.0	36.0
18	35.08946206173058	36.0	36.0	36.0	36.0	36.0
19	35.08531447935854	36.0	36.0	36.0	36.0	36.0
20	35.082288070771774	36.0	36.0	36.0	36.0	36.0
21	35.0701952150676	36.0	36.0	36.0	36.0	36.0
22	35.042303088719684	36.0	36.0	36.0	36.0	36.0
23	35.00928337979283	36.0	36.0	36.0	36.0	36.0
24	34.997683414599415	36.0	36.0	36.0	36.0	36.0
25	34.976023614932025	36.0	36.0	36.0	36.0	36.0
26	34.930430938433105	36.0	36.0	36.0	32.0	36.0
27	34.91134268851693	36.0	36.0	36.0	32.0	36.0
28	34.88444638355279	36.0	36.0	36.0	32.0	36.0
29	34.852807072309474	36.0	36.0	36.0	32.0	36.0
30	34.83067142032181	36.0	36.0	36.0	32.0	36.0
31	34.82771864105827	36.0	36.0	36.0	32.0	36.0
32	34.80007265571233	36.0	36.0	36.0	32.0	36.0
33	34.78949528620205	36.0	36.0	36.0	32.0	36.0
34	34.78101878643054	36.0	36.0	36.0	32.0	36.0
35	34.76815708238026	36.0	36.0	36.0	32.0	36.0
36	34.73001131214148	36.0	36.0	36.0	32.0	36.0
37	34.69644181731774	36.0	36.0	36.0	32.0	36.0
38	34.68290467071567	36.0	36.0	36.0	32.0	36.0
39	34.62942818007177	36.0	36.0	36.0	32.0	36.0
40	34.63970433871436	36.0	36.0	36.0	32.0	36.0
41	34.615272486304036	36.0	36.0	36.0	32.0	36.0
42	34.59431551199458	36.0	36.0	36.0	32.0	36.0
43	34.58758877353628	36.0	36.0	36.0	32.0	36.0
44	34.53760237366334	36.0	36.0	36.0	32.0	36.0
45	34.522863730377935	36.0	36.0	36.0	32.0	36.0
46	34.47298077666157	36.0	36.0	36.0	32.0	36.0
47	34.46092990792683	36.0	36.0	36.0	32.0	36.0
48	34.43750817681017	36.0	36.0	36.0	32.0	36.0
49	34.40751578923028	36.0	36.0	36.0	32.0	36.0
50	33.97278727149809	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	21.0
21	60.0
22	198.0
23	514.0
24	1386.0
25	3381.0
26	7353.0
27	14630.0
28	26767.0
29	43673.0
30	68591.0
31	106042.0
32	166025.0
33	290014.0
34	646792.0
35	1911280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04278634885785	18.075860576646534	12.5203033303092	26.36104974418641
2	15.643168626763693	19.555999381726615	38.406643761341606	26.39418823016809
3	18.894043287414323	23.060069753240988	28.272242706958806	29.773644252385882
4	12.598163586759254	16.061116007902072	35.72480636546991	35.61591403986877
5	14.5902633405269	36.642100225360565	33.45948697384302	15.308149460269513
6	34.911942926895165	35.3670758674574	16.320223249111883	13.40075795653555
7	30.902196527008268	29.873972155945687	19.943688780412412	19.280142536633633
8	27.567487968299233	34.65656180268923	18.521973484924548	19.253976744086987
9	26.98396036916891	14.575806864808651	18.445058224973483	39.995174541048954
10	15.985382449568478	27.11548911472605	31.532883403402888	25.36624503230258
11	36.992680271661776	21.07146486451292	22.57000414393133	19.365850719893974
12	24.9205442241447	23.13685865664821	29.113429927703304	22.829167191503785
13	29.74956293998845	19.975665812931624	24.87521046729063	25.399560779789297
14	22.82067852159621	20.04223645722471	25.1442617504185	31.992823270760578
15	24.698439240899933	28.513655196921928	21.752688230930765	25.035217331247374
16	25.00790302298616	26.203107842871777	24.61842622896952	24.170562905172545
17	23.774725913323074	26.656796686315353	25.420828092568488	24.14764930779309
18	24.18383140198051	25.686024007455437	26.737951318773163	23.39219327179089
19	25.537022466679687	25.095063975362773	25.21268834046199	24.155225217495545
20	25.9307060980155	25.001422388547873	25.202838692011042	23.865032821425583
21	25.832300393034547	24.610540433147314	24.648024452237387	24.909134721580756
22	25.64125968210388	25.474437541948937	24.947683627576797	23.93661914837039
23	24.130663568147195	25.435790151369247	25.52061607719038	24.912930203293183
24	24.819446612271697	25.056462391924306	25.771282510943344	24.352808484860656
25	24.668081852808672	25.172839014107947	25.717118752413114	24.441960380670267
26	23.86191939801346	25.975608494552926	25.967697867599227	24.194774239834388
27	24.57239349200192	25.731297872502264	25.259004023762255	24.437304611733566
28	24.09623927832407	25.568027096011388	25.38182112482402	24.953912500840513
29	24.277098224703725	25.461349144846274	25.227316360175607	25.034236270274395
30	24.27633792823531	25.44803488985562	25.671238445047685	24.604388736861385
31	25.101651494499077	24.978945574919674	25.117533589718626	24.801869340862623
32	24.5377504906899	25.65181660991773	25.152044650270682	24.65838824912169
33	24.095482310044897	25.483125446493226	25.771010891087904	24.650381352373973
34	24.497856824692885	25.600265554146517	25.808680529355783	24.093197091804818
35	24.755433137322854	25.538521733918433	25.655933300250794	24.050111828507916
36	23.97883973935164	25.684913897625204	25.837010497068967	24.499235865954187
37	24.78103977582173	25.424274417571585	24.932469642525717	24.86221616408097
38	24.53788472082012	25.682516443375096	25.176086656236862	24.603512179567925
39	25.183078159290552	25.165005380759066	25.048658065768485	24.603258394181903
40	25.481953187514645	25.1405196045918	25.394693205709018	23.982834002184543
41	24.854344650571683	25.177191569530137	25.842996783701082	24.125466996197094
42	25.015068897035846	25.806805938469	25.405932201069138	23.772192963426015
43	24.511864584182746	25.36125611120104	25.69509777313128	24.431781531484933
44	24.045676452377005	25.74908073553328	25.415787463925433	24.789455348164285
45	24.330064971694686	25.933637228288152	25.15865641188537	24.577641388131788
46	24.49705034988397	25.188451136274782	25.292964501054417	25.021534012786837
47	25.047943327837434	25.219696515616153	25.346480489659363	24.38587966688705
48	24.721520889964772	25.607877071820685	24.909039792466505	24.76156224574804
49	24.804099294897423	25.424372926057032	25.05323107611663	24.71829670292891
50	24.463577553289305	26.17712939675941	24.74939772086256	24.60989532908873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	5.5
2	9.0
3	40.0
4	71.0
5	109.5
6	148.0
7	155.0
8	162.0
9	204.5
10	247.0
11	409.0
12	571.0
13	1085.5
14	1600.0
15	2277.5
16	2955.0
17	3290.0
18	3625.0
19	4210.5
20	4796.0
21	6277.5
22	7759.0
23	9819.0
24	11879.0
25	16273.0
26	20667.0
27	27632.0
28	34597.0
29	42342.5
30	50088.0
31	60229.5
32	70371.0
33	84461.5
34	98552.0
35	115395.0
36	132238.0
37	143308.5
38	154379.0
39	158652.5
40	162926.0
41	169086.5
42	175247.0
43	179047.5
44	182848.0
45	193550.0
46	204252.0
47	213665.0
48	223078.0
49	230485.0
50	237892.0
51	234041.5
52	230191.0
53	228196.0
54	226201.0
55	222917.5
56	219634.0
57	210865.0
58	202096.0
59	185230.0
60	168364.0
61	149157.5
62	129951.0
63	113841.5
64	97732.0
65	82747.5
66	67763.0
67	59922.0
68	52081.0
69	45867.5
70	39654.0
71	32627.5
72	25601.0
73	21776.5
74	17952.0
75	14157.5
76	10363.0
77	8759.5
78	7156.0
79	5991.0
80	4826.0
81	3843.5
82	2861.0
83	2224.5
84	1588.0
85	1299.0
86	1010.0
87	706.5
88	403.0
89	277.0
90	151.0
91	111.5
92	72.0
93	59.0
94	46.0
95	41.0
96	36.0
97	33.0
98	30.0
99	21.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02869109578079638
2	0.004928905107623556
3	1.521267008525789E-4
4	3.0425340170515777E-5
5	3.0425340170515777E-5
6	6.0850680341031553E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.172307828987682E-4
17	0.0
18	1.521267008525789E-4
19	0.0
20	2.7382806153464196E-4
21	0.0
22	0.0
23	9.127602051154733E-5
24	0.0010040362256270208
25	1.521267008525789E-4
26	4.8680544272825243E-4
27	2.7382806153464196E-4
28	0.0015516923486963046
29	4.5638010255773665E-4
30	0.0014604163281847573
31	4.259547623872209E-4
32	0.001064886905968052
33	3.042534017051578E-4
34	6.693574837513471E-4
35	1.521267008525789E-4
36	2.1297738119361044E-4
37	0.0019472217709130097
38	0.0
39	6.997828239218628E-4
40	1.2170136068206311E-4
41	0.0011257375863090837
42	0.004959330447794071
43	0.0044420996648953034
44	0.004229122283701693
45	0.0021906244922771357
46	0.0022210498324476517
47	0.0012474389469911468
48	0.004016144902508083
49	0.0036510408204618932
50	0.0015821176888668202
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3286734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.05625369232733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.35303087658558	28.792097078613548
2	16.412019208485894	14.461041637048078
3	6.976178939975329	9.220329275478727
4	3.6941332883572766	6.5099869330055835
5	2.1460819858188147	4.727416620588365
6	1.3034457409555888	3.44549617426338
7	0.8919716233476107	2.7507849687191563
8	0.6128397659243866	2.159953936024902
9	0.4299380921054878	1.7047315492015107
>10	2.009084037525617	15.210384260878376
>50	0.10460423594056889	3.1699015099407584
>100	0.05953031014468785	5.038736549932198
>500	0.0052004088603403815	1.5729201724555806
>1k	0.0019414859729925886	1.2362193338497378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.0425340170515777E-5	0.0
5	0.0	0.0	0.0	3.0425340170515777E-5	3.0425340170515777E-5
6	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
7	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
8	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
9	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
10	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
11	0.0	0.0	0.0	3.0425340170515777E-5	6.0850680341031553E-5
12	0.0	0.0	0.0	3.0425340170515777E-5	1.2170136068206311E-4
13	0.0	0.0	0.0	9.127602051154733E-5	1.8255204102309467E-4
14	0.0	0.0	0.0	1.2170136068206311E-4	2.4340272136412621E-4
15	0.0	0.0	0.0	1.2170136068206311E-4	2.4340272136412621E-4
16	0.0	0.0	0.0	1.521267008525789E-4	2.4340272136412621E-4
17	0.0	0.0	0.0	1.521267008525789E-4	2.73828061534642E-4
18	0.0	0.0	0.0	1.521267008525789E-4	3.042534017051578E-4
19	0.0	0.0	0.0	1.521267008525789E-4	3.042534017051578E-4
20	0.0	0.0	0.0	1.8255204102309467E-4	3.6510408204618933E-4
21	0.0	0.0	0.0	1.8255204102309467E-4	3.955294222167051E-4
22	0.0	0.0	0.0	1.8255204102309467E-4	4.259547623872209E-4
23	0.0	0.0	0.0	1.8255204102309467E-4	5.172307828987682E-4
24	0.0	0.0	0.0	2.1297738119361044E-4	5.172307828987682E-4
25	0.0	0.0	0.0	2.1297738119361044E-4	5.780814632397997E-4
26	0.0	0.0	0.0	3.042534017051578E-4	6.085068034103156E-4
27	0.0	0.0	0.0	4.259547623872209E-4	6.085068034103156E-4
28	0.0	0.0	0.0	5.780814632397997E-4	6.389321435808313E-4
29	0.0	0.0	0.0	6.693574837513471E-4	6.389321435808313E-4
30	0.0	0.0	0.0	0.001095312246138568	6.389321435808313E-4
31	0.0	0.0	0.0	0.0021297738119361045	7.910588444334102E-4
32	0.0	0.0	0.0	0.0032859367384157037	8.519095247744418E-4
33	0.0	0.0	0.0	0.004776778406770977	8.519095247744418E-4
34	0.0	0.0	0.0	0.006176344054614703	9.127602051154733E-4
35	0.0	0.0	0.0	0.007880163104163587	9.127602051154733E-4
36	0.0	0.0	0.0	0.010101212936611238	9.127602051154733E-4
37	0.0	0.0	0.0	0.013721828416902615	9.127602051154733E-4
38	0.0	0.0	0.0	0.019837321791176286	9.127602051154733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3530	0.0	27.746546	1
GGTATCA	1500	0.0	25.53181	1
TCCGTTA	100	4.6306013E-8	21.999802	26
GTAGGAC	2810	0.0	20.90341	3
TAGGACG	2820	0.0	20.829283	4
GTCCTAC	2490	0.0	19.446749	1
AGGACGT	2980	0.0	19.194166	5
TGTAGGA	3050	0.0	19.187588	2
GTCCTAA	1625	0.0	18.827171	1
GGACGTG	3010	0.0	18.71051	6
TCAACGC	5180	0.0	18.644337	4
CTGTAGG	3115	0.0	18.44192	1
TATCAAC	5390	0.0	18.327202	2
ATCAACG	5310	0.0	18.312174	3
ATACCGT	640	0.0	18.21831	6
CAACGCA	5295	0.0	18.114763	5
TTAGGAC	1850	0.0	18.075237	3
TAGGACC	1620	0.0	17.925493	4
AACGCAG	5415	0.0	17.835209	6
TACCGTC	560	0.0	17.677876	7
>>END_MODULE