FastQCFastQC Report
Thu 2 Feb 2017
SRR4061996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813752
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC35990.442272338501165No Hit
GTATCAACGCAGAGTACTTTTTTTT31150.3827947581081214No Hit
CCCATGTACTCTGCGTTGATACCAC23160.2846075954344813No Hit
GTCCTAAAGTGTGTATTTCTCATTT20010.2458980131538847No Hit
TATCAACGCAGAGTACTTTTTTTTT19190.23582123300465008No Hit
GTCCTACAGTGGACATTTCTAAATT18410.22623600310659758No Hit
CTTTAGGACGTGAAATATGGCGAGG18320.2251300150414377No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17470.21468457220381643No Hit
CTGTAGGACGTGGAATATGGCAAGA17370.21345569657586094No Hit
GTATCAACGCAGAGTACATGGGAAG15970.19625143778448473No Hit
GTCCTACAGTGTGCATTTCTCATTT13380.16442355902043868No Hit
GTACATGGAAGCAGTGGTATCAACG11820.14525309922433371No Hit
GGTATCAACGCAGAGTACTTTTTTT11360.13960027133573866No Hit
GAGTACATGGGAAGCAGTGGTATCA10900.1339474434471436No Hit
TCCATGTACTCTGCGTTGATACCAC9920.12190446229318024No Hit
CTGTAGGACCTGGAATATGGCGAGA9800.1204298115396337No Hit
GCGTTGATACCACTGCTTCCCATGT9790.12030692397683815No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9340.11477698365103864No Hit
TATCAACGCAGAGTACATGGGAAGC9210.11317944533469657No Hit
CATGTACTCTGCGTTGATACCACTG9030.11096746920437678No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8860.1088783806368525No Hit
ATTTAGAAATGTCCACTGTAGGACG8720.10715795475771488No Hit
CTGAAGGACCTGGAATATGGCGAGA8180.10052202636675549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC250.006024576719.00277111
TACCGAG250.00602886419.000435
ATAAGGT508.717821E-515.1975363
GTATTAG551.9610331E-413.8159431
TAGAAAT3200.013.0619934
ATGTCCA3650.013.0147959
TAGGACC3850.012.5839274
AATGTCC3700.012.3253638
GTGTTAG853.9439055E-612.2921271
GTCCTAT1251.4097168E-912.158031
TTTAGAA3500.011.9409232
ATTTAGA3200.011.8730761
GAAATGT3700.011.8118066
GTTCTAG658.0270926E-411.6904131
GTATAGA658.0270926E-411.6904131
ATAGGAC1001.9294148E-611.3981523
TTAGAAA3350.011.3414463
AAATGTC4000.011.163447
AGGACGT11500.011.1524275
CTAGAAC600.00587865311.0815373
Produced by FastQC (version 0.11.5)