##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061996_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813752
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171102498058378	32.0	32.0	32.0	32.0	32.0
2	30.77268873071894	32.0	32.0	32.0	32.0	32.0
3	30.79697745750548	32.0	32.0	32.0	32.0	32.0
4	30.793454271080133	32.0	32.0	32.0	32.0	32.0
5	30.766320697214876	32.0	32.0	32.0	32.0	32.0
6	34.39047031528033	36.0	36.0	36.0	32.0	36.0
7	34.32408399610692	36.0	36.0	36.0	32.0	36.0
8	34.270404250926575	36.0	36.0	36.0	32.0	36.0
9	34.34557948859112	36.0	36.0	36.0	32.0	36.0
10	34.09884829776148	36.0	36.0	36.0	32.0	36.0
11	34.36939386938527	36.0	36.0	36.0	32.0	36.0
12	34.21606582840964	36.0	36.0	36.0	32.0	36.0
13	34.24126515203649	36.0	36.0	36.0	32.0	36.0
14	34.1852493142874	36.0	36.0	36.0	32.0	36.0
15	34.119751472193	36.0	36.0	36.0	32.0	36.0
16	34.13299629371111	36.0	36.0	36.0	32.0	36.0
17	34.07699520246955	36.0	36.0	36.0	32.0	36.0
18	34.061129189237015	36.0	36.0	36.0	32.0	36.0
19	34.03752248842399	36.0	36.0	36.0	32.0	36.0
20	33.99215731574239	36.0	36.0	36.0	32.0	36.0
21	33.979374551460396	36.0	36.0	36.0	32.0	36.0
22	33.90160884397212	36.0	36.0	36.0	32.0	36.0
23	33.88813545158722	36.0	36.0	36.0	32.0	36.0
24	33.81981242442415	36.0	36.0	36.0	32.0	36.0
25	33.429492031970426	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	11.0
5	55.0
6	176.0
7	30.0
8	142.0
9	107.0
10	59.0
11	14.0
12	52.0
13	39.0
14	240.0
15	319.0
16	456.0
17	581.0
18	781.0
19	1010.0
20	1452.0
21	1988.0
22	2773.0
23	3890.0
24	5482.0
25	7404.0
26	9488.0
27	12418.0
28	16453.0
29	21105.0
30	27985.0
31	37779.0
32	53721.0
33	75472.0
34	165457.0
35	366812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70479081284678	17.734671180775305	11.794603960700218	25.765934045677703
2	14.725143194908515	20.291833742754235	40.31982756395998	24.66319549837727
3	19.00514655642779	25.80295128221513	28.292911318983272	26.898990842373806
4	12.291269032663239	17.111914333504984	36.647055859460934	33.949760774370844
5	13.289489050197606	37.65183131834023	34.770401421046486	14.288278210415681
6	32.21784179809988	35.747785945642846	18.40880752175934	13.62556473449793
7	28.311784172130842	30.785980538093423	22.20933236556891	18.692902924206823
8	26.252401959457632	35.572004655065086	20.07742859322063	18.09816479225665
9	27.28512003700828	14.958950086799682	19.332522136687043	38.42340773950499
10	15.629802906262036	27.956498359090343	33.16520213275187	23.248496601895752
11	34.17622446468127	21.585158749692347	24.727295102141277	19.511321683485107
12	25.554383819043412	23.647680023232375	30.61173259632884	20.18620356139537
13	30.16848381790016	21.481840586967945	24.93444843789797	23.41522715723393
14	22.00799467513789	21.595711566688117	26.559964369684213	29.836329388489784
15	23.98431308850529	30.197181573416422	22.94364623479669	22.8748591032816
16	22.50248223067336	26.271535734894076	28.316130992879966	22.909851041552603
17	22.645203045560365	27.610357267657903	27.367962555553916	22.37647713122782
18	22.937555207259432	25.29790390799733	29.996333868900138	21.7682070158431
19	24.67735348674726	25.57394915396008	26.852603559321196	22.89609379997146
20	25.195509012233614	25.746304455317677	27.435098879959064	21.62308765248965
21	23.61956210233192	25.440302280014908	26.393291194200398	24.546844423452775
22	24.511810771778148	27.411422287065353	25.420899444213447	22.65586749694305
23	21.88285964828132	27.672726244107377	27.338078178019	23.10633592959231
24	23.772969914639248	26.481267373496348	27.532164030405156	22.213598681459253
25	23.028547532071386	26.476144791705103	27.298746663714745	23.196561012508763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	684.0
1	684.0
2	677.0
3	670.0
4	670.0
5	670.0
6	1628.0
7	2586.0
8	2586.0
9	2586.0
10	2435.0
11	2284.0
12	2284.0
13	2284.0
14	2483.0
15	2682.0
16	2682.0
17	2682.0
18	4779.5
19	6877.0
20	6877.0
21	6877.0
22	11131.0
23	15385.0
24	15385.0
25	15385.0
26	23646.5
27	31908.0
28	31908.0
29	31908.0
30	41782.5
31	51657.0
32	51657.0
33	51657.0
34	58391.5
35	65126.0
36	65126.0
37	65126.0
38	72249.0
39	79372.0
40	79372.0
41	79372.0
42	86321.5
43	93271.0
44	93271.0
45	93271.0
46	106516.0
47	119761.0
48	119761.0
49	119761.0
50	112891.0
51	106021.0
52	106021.0
53	106021.0
54	94470.0
55	82919.0
56	82919.0
57	82919.0
58	72527.5
59	62136.0
60	62136.0
61	62136.0
62	52356.0
63	42576.0
64	42576.0
65	42576.0
66	34013.5
67	25451.0
68	25451.0
69	25451.0
70	19091.0
71	12731.0
72	12731.0
73	12731.0
74	9138.5
75	5546.0
76	5546.0
77	5546.0
78	3875.0
79	2204.0
80	2204.0
81	2204.0
82	1581.0
83	958.0
84	958.0
85	958.0
86	691.0
87	424.0
88	424.0
89	424.0
90	292.5
91	161.0
92	161.0
93	161.0
94	108.5
95	56.0
96	56.0
97	56.0
98	181.0
99	306.0
100	306.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.60212939568812E-4
2	1.228875627955446E-4
3	4.915502511821784E-4
4	0.010445442837621291
5	0.032810979266410405
6	0.06611350878400299
7	0.10838683038567032
8	0.10740372988330597
9	0.11809494784651835
10	0.13431610613553024
11	0.14156647234046738
12	0.13431610613553024
13	0.12694285236779757
14	0.11809494784651835
15	0.13517631907509906
16	0.11981537372565598
17	0.12632841455381985
18	0.11133613189276341
19	0.09683539948288913
20	0.09167412184547627
21	0.0898308084035431
22	0.1044544283762129
23	0.08098290388226388
24	0.08995369596633863
25	0.08872482033838319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	813752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.95437563378949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.58821995851807	42.48125497373152
2	14.982783988527896	17.066702146450886
3	4.86626659097165	8.314655260690786
4	2.090068563794158	4.761542003308295
5	1.0739905334797333	3.0584230135469346
6	0.6231021259945547	2.1293035525264017
7	0.3827693114903017	1.526027100339393
8	0.2678189280259919	1.2202767862904937
9	0.19538350060403203	1.0015150757442097
>10	0.8484716956404869	8.174490853640684
>50	0.04338300107715099	1.6747314038876575
>100	0.03015006013394488	3.3604799877906286
>500	0.004555045045239677	1.9630184154512855
>1k	0.0030366966968264518	3.267579426600787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3599	0.442272338501165	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3115	0.3827947581081214	No Hit
CCCATGTACTCTGCGTTGATACCAC	2316	0.2846075954344813	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2001	0.2458980131538847	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1919	0.23582123300465008	No Hit
GTCCTACAGTGGACATTTCTAAATT	1841	0.22623600310659758	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1832	0.2251300150414377	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1747	0.21468457220381643	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1737	0.21345569657586094	No Hit
GTATCAACGCAGAGTACATGGGAAG	1597	0.19625143778448473	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1338	0.16442355902043868	No Hit
GTACATGGAAGCAGTGGTATCAACG	1182	0.14525309922433371	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1136	0.13960027133573866	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1090	0.1339474434471436	No Hit
TCCATGTACTCTGCGTTGATACCAC	992	0.12190446229318024	No Hit
CTGTAGGACCTGGAATATGGCGAGA	980	0.1204298115396337	No Hit
GCGTTGATACCACTGCTTCCCATGT	979	0.12030692397683815	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	934	0.11477698365103864	No Hit
TATCAACGCAGAGTACATGGGAAGC	921	0.11317944533469657	No Hit
CATGTACTCTGCGTTGATACCACTG	903	0.11096746920437678	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	886	0.1088783806368525	No Hit
ATTTAGAAATGTCCACTGTAGGACG	872	0.10715795475771488	No Hit
CTGAAGGACCTGGAATATGGCGAGA	818	0.10052202636675549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.915502511821784E-4
13	0.0	0.0	0.0	0.0	7.373253767732676E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	25	0.0060245767	19.002771	11
TACCGAG	25	0.006028864	19.00043	5
ATAAGGT	50	8.717821E-5	15.197536	3
GTATTAG	55	1.9610331E-4	13.815943	1
TAGAAAT	320	0.0	13.061993	4
ATGTCCA	365	0.0	13.014795	9
TAGGACC	385	0.0	12.583927	4
AATGTCC	370	0.0	12.325363	8
GTGTTAG	85	3.9439055E-6	12.292127	1
GTCCTAT	125	1.4097168E-9	12.15803	1
TTTAGAA	350	0.0	11.940923	2
ATTTAGA	320	0.0	11.873076	1
GAAATGT	370	0.0	11.811806	6
GTTCTAG	65	8.0270926E-4	11.690413	1
GTATAGA	65	8.0270926E-4	11.690413	1
ATAGGAC	100	1.9294148E-6	11.398152	3
TTAGAAA	335	0.0	11.341446	3
AAATGTC	400	0.0	11.16344	7
AGGACGT	1150	0.0	11.152427	5
CTAGAAC	60	0.005878653	11.081537	3
>>END_MODULE