FastQCFastQC Report
Thu 2 Feb 2017
SRR4061996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813752
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.13701963251703222No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8560.10519175375298616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGCG300.005747179529.3299317
TAGGACC5950.024.4005324
GTCCGGC500.002575015922.00691640
TCCGAAT654.934113E-420.30533811
TTAGGAC8550.020.067853
GTATTAT909.985863E-619.5617031
ACGGAAA7550.019.2366538
GTAGGAC15450.019.2210733
ATCACGG6650.019.18692435
CTGTAGG15400.019.1488741
GTCCTAC13700.019.1154981
TAGGACG15200.019.1030484
TCCTACA14050.019.1021632
CACGGAA7200.018.94690537
TGTAGGA15700.018.9161682
AACGCAT1051.7921539E-618.85611723
ACGCATA708.12318E-418.85611524
CTTTAGG9200.018.6580371
AGGACGT15450.018.651565
GGACGTG15300.018.546876
Produced by FastQC (version 0.11.5)