##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813752
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145990424601106	32.0	32.0	32.0	32.0	32.0
2	31.43779185796164	32.0	32.0	32.0	32.0	32.0
3	31.57483115248872	32.0	32.0	32.0	32.0	32.0
4	31.664600517110863	32.0	32.0	32.0	32.0	32.0
5	31.644045114482054	32.0	32.0	32.0	32.0	32.0
6	35.2867138882608	36.0	36.0	36.0	36.0	36.0
7	35.300782056449634	36.0	36.0	36.0	36.0	36.0
8	35.260948052969454	36.0	36.0	36.0	36.0	36.0
9	35.32755679863153	36.0	36.0	36.0	36.0	36.0
10	35.22257518261092	36.0	36.0	36.0	36.0	36.0
11	35.33200778615598	36.0	36.0	36.0	36.0	36.0
12	35.26297692663121	36.0	36.0	36.0	36.0	36.0
13	35.266431296021395	36.0	36.0	36.0	36.0	36.0
14	35.242362537972255	36.0	36.0	36.0	36.0	36.0
15	35.2018305331354	36.0	36.0	36.0	36.0	36.0
16	35.21889347122956	36.0	36.0	36.0	36.0	36.0
17	35.1950434530422	36.0	36.0	36.0	36.0	36.0
18	35.18458080594579	36.0	36.0	36.0	36.0	36.0
19	35.16135628545306	36.0	36.0	36.0	36.0	36.0
20	35.14511054965149	36.0	36.0	36.0	36.0	36.0
21	35.11954993659002	36.0	36.0	36.0	36.0	36.0
22	35.062727956428986	36.0	36.0	36.0	36.0	36.0
23	35.04576824388757	36.0	36.0	36.0	36.0	36.0
24	35.02100025560613	36.0	36.0	36.0	36.0	36.0
25	34.96469317433321	36.0	36.0	36.0	36.0	36.0
26	34.907573806270214	36.0	36.0	36.0	36.0	36.0
27	34.839017292737836	36.0	36.0	36.0	32.0	36.0
28	34.784541236150574	36.0	36.0	36.0	32.0	36.0
29	34.724391460789036	36.0	36.0	36.0	32.0	36.0
30	34.694068954669234	36.0	36.0	36.0	32.0	36.0
31	34.6508211346946	36.0	36.0	36.0	32.0	36.0
32	34.6359984368702	36.0	36.0	36.0	32.0	36.0
33	34.6378798454566	36.0	36.0	36.0	32.0	36.0
34	34.595025265682914	36.0	36.0	36.0	32.0	36.0
35	34.55215962602857	36.0	36.0	36.0	32.0	36.0
36	34.45712084271375	36.0	36.0	36.0	32.0	36.0
37	34.373675272073065	36.0	36.0	36.0	32.0	36.0
38	34.33944862808325	36.0	36.0	36.0	32.0	36.0
39	34.18186499080801	36.0	36.0	36.0	32.0	36.0
40	34.164902820515344	36.0	36.0	36.0	32.0	36.0
41	34.123211985961326	36.0	36.0	36.0	32.0	36.0
42	34.07995064835478	36.0	36.0	36.0	32.0	36.0
43	34.0517258329319	36.0	36.0	36.0	32.0	36.0
44	34.02657566433017	36.0	36.0	36.0	32.0	36.0
45	33.923652414986385	36.0	36.0	36.0	32.0	36.0
46	33.84464554311387	36.0	36.0	36.0	32.0	36.0
47	33.81652763987062	36.0	36.0	36.0	32.0	36.0
48	33.77443250523501	36.0	36.0	36.0	32.0	36.0
49	33.7614334588425	36.0	36.0	36.0	32.0	36.0
50	33.39511915178089	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	21.0
21	40.0
22	128.0
23	369.0
24	913.0
25	2285.0
26	4473.0
27	7655.0
28	11589.0
29	15597.0
30	20687.0
31	28297.0
32	39976.0
33	60751.0
34	130906.0
35	490057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.12918262682487	18.350084328640758	12.315177680200225	26.20555536433415
2	14.354657343172978	20.688324536231974	39.87833578095525	25.0786823396398
3	18.42398577448329	26.056683326185347	28.60562655069315	26.913704348638216
4	12.089186877574495	17.355041830926375	36.74927987888202	33.80649141261711
5	13.080921658986174	37.89960061443932	34.70734254992319	14.312135176651305
6	32.37222734254993	35.97665130568357	18.241720430107527	13.409400921658987
7	28.417994671595277	30.631076789980238	22.355951198891063	18.59497733953342
8	26.39809180192491	35.54313845004375	20.09678624445777	17.96198350357357
9	27.32822776472439	14.616369606464868	19.28179592799772	38.773606700813026
10	15.446597980711568	27.93135992292492	33.33214542022631	23.2898966761372
11	34.38652070901208	21.334264001808904	24.83361023997483	19.44560504920418
12	25.456650183348245	23.590725429860697	30.800170076386905	20.152454310404153
13	30.33811284027566	21.139241439652377	25.12743440261898	23.395211317452983
14	22.15060608145971	21.29002447920251	26.692038852131855	29.86733058720593
15	24.21511713642486	30.01516432524897	22.927624141015936	22.842094397310237
16	22.677536534652003	26.083627855306556	28.38593766129072	22.85289794875072
17	22.73444489230134	27.49977880238697	27.429610004030714	22.33616630128098
18	23.114715821812595	24.89536098310292	30.125345622119816	21.86457757296467
19	24.91864843342935	25.42150434038872	27.008351438767587	22.651495787414348
20	25.359324999877114	25.352074598032807	27.54280194851477	21.745798453575308
21	23.711155241400327	25.13714252007983	26.442454212094102	24.709248026425744
22	24.48313491088194	27.171669009722866	25.63815511359726	22.707040965797933
23	21.97023413796112	27.247524119786025	27.51824575330783	23.263995988945023
24	23.92346496752668	26.13349390779667	27.74149149917972	22.20154962549693
25	23.489003392929778	25.91198044610698	27.404083067179023	23.19493309378422
26	22.318539591641116	26.529809706972085	28.035441077978973	23.116209623407823
27	23.037362734592357	26.985740127213205	26.625802455785053	23.351094682409382
28	22.54338598720479	26.6858537472565	26.67147572572142	24.09928453981729
29	23.67305357432522	25.82654581025084	26.698437354070304	23.801963261353638
30	22.80636860331653	26.08910440901416	27.73225420342074	23.372272784248572
31	24.90678955453149	24.817695852534563	26.905683563748077	23.369831029185868
32	22.530276683727703	26.65884275786641	27.47482319399812	23.33605736440777
33	22.15968579953499	27.380098015601884	27.993924409030807	22.46629177583232
34	22.546934120801673	26.690236646033718	28.062776268299604	22.700052964865
35	22.33462365591398	27.3673732718894	27.866666666666667	22.431336405529954
36	22.74352748025187	26.50083318177126	28.144216637091578	22.611422700885285
37	22.858038194017745	26.73863887728267	26.32302701108462	24.080295917614965
38	23.441171265938518	27.68251260826394	26.326448352815106	22.54986777298243
39	24.31133471295782	26.015302034536493	26.38568696943034	23.28767628307535
40	24.270937915898106	26.042364310458606	27.447585317867507	22.239112455775782
41	24.092555247922864	25.93589360742546	27.22893402200454	22.742617122647133
42	23.435145836764086	27.197505769812146	27.323470784719028	22.043877608704733
43	22.53270150567023	27.16473890410016	27.26944271030472	23.03311687992489
44	22.141385938091425	27.495465248317597	27.156280569633633	23.206868243957352
45	23.004843111929834	27.228095702152917	26.55182965419861	23.215231531718636
46	22.85117390796758	26.145981185521084	27.41433021806853	23.588514688442796
47	24.41953631722921	26.21383085139589	26.656229339376168	22.710403491998733
48	22.577897938492733	27.23880918000553	26.398844818581217	23.784448062920518
49	23.586968142956685	26.52639883794236	26.489654418117087	23.396978600983864
50	22.647350142919993	28.65367647239543	25.766889259740804	22.932084124943778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	407.0
1	282.0
2	157.0
3	449.5
4	742.0
5	659.5
6	577.0
7	496.0
8	415.0
9	550.0
10	685.0
11	889.0
12	1093.0
13	1770.0
14	2447.0
15	3040.5
16	3634.0
17	3336.5
18	3039.0
19	2882.0
20	2725.0
21	3018.5
22	3312.0
23	3692.0
24	4072.0
25	4940.5
26	5809.0
27	7610.5
28	9412.0
29	12450.5
30	15489.0
31	19826.0
32	24163.0
33	29586.0
34	35009.0
35	40620.0
36	46231.0
37	46532.0
38	46833.0
39	46043.5
40	45254.0
41	45089.5
42	44925.0
43	46689.0
44	48453.0
45	50766.0
46	53079.0
47	54875.5
48	56672.0
49	56681.0
50	56690.0
51	55261.5
52	53833.0
53	52048.0
54	50263.0
55	48360.5
56	46458.0
57	43507.5
58	40557.0
59	37306.0
60	34055.0
61	29851.0
62	25647.0
63	21808.0
64	17969.0
65	15157.0
66	12345.0
67	10240.0
68	8135.0
69	6612.5
70	5090.0
71	4048.0
72	3006.0
73	2510.5
74	2015.0
75	1604.5
76	1194.0
77	967.0
78	740.0
79	620.0
80	500.0
81	407.0
82	314.0
83	235.0
84	156.0
85	116.5
86	77.0
87	52.0
88	27.0
89	19.5
90	12.0
91	12.0
92	12.0
93	8.5
94	5.0
95	10.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032933866829205946
2	0.00454683982343515
3	3.6866268838663374E-4
4	0.0
5	2.457751255910892E-4
6	2.457751255910892E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.915502511821784E-4
17	0.0
18	2.457751255910892E-4
19	0.0
20	4.915502511821784E-4
21	0.0
22	0.0
23	1.228875627955446E-4
24	8.60212939568812E-4
25	1.228875627955446E-4
26	8.60212939568812E-4
27	0.0
28	0.0012288756279554458
29	4.915502511821784E-4
30	0.0012288756279554458
31	2.457751255910892E-4
32	8.60212939568812E-4
33	4.915502511821784E-4
34	6.144378139777229E-4
35	2.457751255910892E-4
36	4.915502511821784E-4
37	0.001474650753546535
38	0.0
39	4.915502511821784E-4
40	1.228875627955446E-4
41	0.0013517631907509905
42	0.004178177135048516
43	0.003440851758275248
44	0.0044239522606396055
45	0.002334863693115347
46	0.002089088567524258
47	7.373253767732675E-4
48	0.003317964195479704
49	0.0028264139442975254
50	0.0012288756279554458
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	813752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.03483619065571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.426923656832	47.54523777073499
2	14.677020915201066	18.50327218313054
3	4.7625270499996155	9.006153373508777
4	1.984704061798295	5.004219816895382
5	1.0183605338472734	3.209609471704579
6	0.5957140668742958	2.253044317313434
7	0.3589432651553841	1.5838150944566114
8	0.24196026836874096	1.2201540705016545
9	0.16982662563053333	0.9634494174689232
>10	0.7246826042086993	7.426244681630462
>50	0.025834024442863507	1.1083105628503018
>100	0.011741676229794658	1.3635747066202861
>500	0.0015655568102057003	0.6753728481308597
>1k	1.9569460127571254E-4	0.137541685053134
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	0.13701963251703222	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	856	0.10519175375298616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.457751255910892E-4
2	0.0	0.0	0.0	0.0	2.457751255910892E-4
3	0.0	0.0	0.0	0.0	2.457751255910892E-4
4	0.0	0.0	0.0	0.0	2.457751255910892E-4
5	0.0	0.0	0.0	0.0	2.457751255910892E-4
6	0.0	0.0	0.0	0.0	2.457751255910892E-4
7	0.0	0.0	0.0	0.0	2.457751255910892E-4
8	0.0	0.0	0.0	0.0	2.457751255910892E-4
9	0.0	0.0	0.0	0.0	2.457751255910892E-4
10	0.0	0.0	0.0	0.0	2.457751255910892E-4
11	0.0	0.0	0.0	0.0	3.686626883866338E-4
12	0.0	0.0	0.0	0.0	9.831005023643567E-4
13	0.0	0.0	0.0	1.228875627955446E-4	9.831005023643567E-4
14	0.0	0.0	0.0	2.457751255910892E-4	9.831005023643567E-4
15	0.0	0.0	0.0	3.686626883866338E-4	9.831005023643567E-4
16	0.0	0.0	0.0	6.144378139777229E-4	9.831005023643567E-4
17	0.0	0.0	0.0	0.0011059880651599014	9.831005023643567E-4
18	0.0	0.0	0.0	0.0013517631907509905	9.831005023643567E-4
19	0.0	0.0	0.0	0.0019662010047287134	9.831005023643567E-4
20	0.0	0.0	0.0	0.0025806388187064365	0.0011059880651599014
21	0.0	0.0	0.0	0.0038095144466618823	0.0014746507535465352
22	0.0	0.0	0.0	0.005529940325799507	0.0014746507535465352
23	0.0	0.0	0.0	0.008479241832892576	0.0014746507535465352
24	0.0	0.0	0.0	0.011551430902781192	0.0014746507535465352
25	0.0	0.0	0.0	0.013763407033100994	0.0014746507535465352
26	0.0	0.0	0.0	0.015852495600625252	0.0014746507535465352
27	0.0	0.0	0.0	0.018801797107718322	0.0014746507535465352
28	0.0	0.0	0.0	0.023225749368357928	0.0014746507535465352
29	0.0	0.0	0.0	0.0283870270057708	0.0014746507535465352
30	0.0	0.0	0.0	0.035391618085116844	0.0014746507535465352
31	0.0	0.0	0.0	0.044362410169191596	0.0018433134419331687
32	0.0	0.0	0.0	0.05640539132315497	0.0018433134419331687
33	0.0	0.0	0.0	0.06758815953754953	0.0018433134419331687
34	0.0	0.0	0.0	0.07754205212398864	0.0019662010047287134
35	0.0	0.0	0.0	0.09351743528740943	0.0019662010047287134
36	0.0	0.0	0.0	0.10740372988330597	0.0019662010047287134
37	0.0	0.0	0.0	0.12460798867468222	0.0019662010047287134
38	0.0	0.0	0.0	0.15311790324324856	0.0019662010047287134
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAGCG	30	0.0057471795	29.329931	7
TAGGACC	595	0.0	24.400532	4
GTCCGGC	50	0.0025750159	22.006916	40
TCCGAAT	65	4.934113E-4	20.305338	11
TTAGGAC	855	0.0	20.06785	3
GTATTAT	90	9.985863E-6	19.561703	1
ACGGAAA	755	0.0	19.23665	38
GTAGGAC	1545	0.0	19.221073	3
ATCACGG	665	0.0	19.186924	35
CTGTAGG	1540	0.0	19.148874	1
GTCCTAC	1370	0.0	19.115498	1
TAGGACG	1520	0.0	19.103048	4
TCCTACA	1405	0.0	19.102163	2
CACGGAA	720	0.0	18.946905	37
TGTAGGA	1570	0.0	18.916168	2
AACGCAT	105	1.7921539E-6	18.856117	23
ACGCATA	70	8.12318E-4	18.856115	24
CTTTAGG	920	0.0	18.658037	1
AGGACGT	1545	0.0	18.65156	5
GGACGTG	1530	0.0	18.54687	6
>>END_MODULE