FastQCFastQC Report
Thu 2 Feb 2017
SRR4061995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187907
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12300.6545791269085239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA8030.427339056022394No Hit
TATCAACGCAGAGTACTTTTTTTTT7320.3895544072333655No Hit
GGTATCAACGCAGAGTACTTTTTTT5310.28258659868977737No Hit
CCATAGGGTCTTCTCGTCTTATTAT4560.2426732372929162No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4370.23256185240571134No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3700.1969059162245153No Hit
GTATTAGAGGCACTGCCTGCCCAGT3170.1687004741707334No Hit
GTCCTAAAGTGTGTATTTCTCATTT3130.1665717615629008No Hit
CTGTAGGACCTGGAATATGGCGAGA2990.1591212674354867No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2800.14900988254828187No Hit
GTATCAACGCAGAGTACATGGGGTG2730.14528463548457482No Hit
GTGTATATCAATGAGTTACAATGAA2540.13517325059736998No Hit
GTACATGGGGTGGTATCAACGCAAA2490.13251235983757922No Hit
GTCCTACAGTGTGCATTTCTCATTT2470.13144800353366293No Hit
ATAAATAATCCACCTATAACTTCTC2390.12719057831799774No Hit
GTCCTACAGTGGACATTTCTAAATT2320.12346533125429067No Hit
CTTTAGGACGTGAAATATGGCGAGG2300.12240097495037439No Hit
CCTATAACTTCTCTGTTAACCCAAC2260.12027226234254179No Hit
ACCTATAACTTCTCTGTTAACCCAA2160.1149504808229603No Hit
CTGTAGGACGTGGAATATGGCAAGA2140.113886124519044No Hit
CCATTGGGATGTCCTGATCCAACAT2140.113886124519044No Hit
GTTATATAATTTAAGCTCCATAGGG2110.11228959006316953No Hit
GTCAGGATACCGCGGCCGTTAAACT2100.11175741191121139No Hit
GATATACACTGTTCTACAAATCCCG2070.11016087745533695No Hit
GGTATCAACGCAGAGTACATGGGGT2060.10962869930337879No Hit
GACTATAGGCAATAATCACACTATA2010.10696780854358805No Hit
CTCTAATACTTGTAATGCTAGAGGT2000.1064356303916299No Hit
ATCCTGACCGTGCAAAGGTAGCATA1890.10058167072009025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC307.6794554E-419.003212
AAGCGTT307.6794554E-419.003211
AAAGCGT307.6794554E-419.003210
AATCGTA307.6794554E-419.003213
GAAAGCG359.887911E-518.9981339
TCAAGCT359.887911E-518.99813317
TTCAAGC402.7413756E-416.62780216
AAGAAAG402.7469636E-416.6233677
AAATCGT350.002158210116.28845812
GCGTTCA350.002158210116.28845813
GTGGCAG350.002162061416.28411517
TGTTGGA350.002162061416.2841152
TAGCATT551.1188313E-515.5439285
TATTACA508.6562475E-515.1985074
GTTCAAG456.714589E-414.78026715
AAGCTCA456.714589E-414.78026719
CCAATAA456.755471E-414.768451
TAGCATA602.5405401E-514.252419
TATCACC400.005259655414.24863
GCATTAC400.005259655414.24867
Produced by FastQC (version 0.11.5)