##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061995_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187907
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06688947191962	32.0	32.0	32.0	32.0	32.0
2	30.688415013810022	32.0	32.0	32.0	32.0	32.0
3	30.748173298493402	32.0	32.0	32.0	32.0	32.0
4	30.71427355021367	32.0	32.0	32.0	32.0	32.0
5	30.691943354957505	32.0	32.0	32.0	32.0	32.0
6	34.31143065452591	36.0	36.0	36.0	32.0	36.0
7	34.156343297482266	36.0	36.0	36.0	32.0	36.0
8	34.107388229283636	36.0	36.0	36.0	32.0	36.0
9	34.20594762302628	36.0	36.0	36.0	32.0	36.0
10	33.93138627086804	36.0	36.0	36.0	32.0	36.0
11	34.30287855162394	36.0	36.0	36.0	32.0	36.0
12	34.06406360593272	36.0	36.0	36.0	32.0	36.0
13	34.187539580750055	36.0	36.0	36.0	32.0	36.0
14	34.10478055633904	36.0	36.0	36.0	32.0	36.0
15	34.05464405264306	36.0	36.0	36.0	32.0	36.0
16	34.05600110693056	36.0	36.0	36.0	32.0	36.0
17	33.95953849510662	36.0	36.0	36.0	32.0	36.0
18	34.01220816680592	36.0	36.0	36.0	32.0	36.0
19	33.9430090417068	36.0	36.0	36.0	32.0	36.0
20	33.89914691842241	36.0	36.0	36.0	32.0	36.0
21	33.83605187672625	36.0	36.0	36.0	32.0	36.0
22	33.85525818623042	36.0	36.0	36.0	32.0	36.0
23	33.832358560351665	36.0	36.0	36.0	32.0	36.0
24	33.8174469285338	36.0	36.0	36.0	32.0	36.0
25	33.46854561032851	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	11.0
6	39.0
7	7.0
8	25.0
9	31.0
10	12.0
11	0.0
12	10.0
13	9.0
14	62.0
15	79.0
16	115.0
17	162.0
18	215.0
19	294.0
20	458.0
21	595.0
22	831.0
23	1089.0
24	1436.0
25	1862.0
26	2498.0
27	3056.0
28	3833.0
29	4897.0
30	6339.0
31	8593.0
32	12095.0
33	17295.0
34	38492.0
35	83462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.715734014528614	20.417764295787766	12.738351826720951	24.128149862962665
2	14.76208975716711	21.544168125721765	41.227309254045885	22.46643286306524
3	17.94248187923749	27.062999584898833	29.78297659468032	25.211541941183356
4	10.878231296137614	18.111757473827634	39.02995917802532	31.980052052009434
5	11.836634743863423	39.950172745130395	34.478224531405544	13.734967979600638
6	28.4707836987257	40.18765742402377	18.355175223255888	12.986383653994643
7	27.159737464572636	33.675709080060514	20.63151277516142	18.533040680205424
8	25.844809674755602	35.7694254281985	19.22964225779814	19.156122639247755
9	26.989471889518775	15.360598439964196	20.570308171007206	37.07962149950983
10	15.603169443748769	27.977747819275635	32.374365232033334	24.044717504942266
11	33.145211144507684	23.976785828483724	24.295474408987232	18.58252861802136
12	23.212315956069254	27.41461906969557	29.900511033310416	19.47255394092476
13	27.666116054777	22.171364629402674	26.779453295678586	23.383066020141737
14	21.495894760958404	23.397997730263683	27.35671623500828	27.749391273769625
15	23.890881379597015	30.322037420929504	24.11203896636806	21.675042233105426
16	22.3651262160751	28.299253039521776	26.96996174624655	22.365658998156572
17	19.919860181593418	28.964363357346862	27.23847990110405	23.877296559955667
18	20.087371140885963	28.171865427133003	30.408353533470073	21.332409898510964
19	23.62883808143871	26.788796317998763	27.83927467984914	21.74309092071339
20	23.664825536493257	27.22227252632532	28.30724325821451	20.805658678966907
21	24.544723371843425	25.418257937712724	26.985826369025744	23.05119232141811
22	23.173869271784927	27.877890408186097	27.43310978890233	21.51513053112665
23	22.43478446117958	27.726590044844006	27.91778954207987	21.92083595189655
24	22.472143511515437	28.051963269914992	28.014146622067877	21.461746596501694
25	21.864014998189138	27.94797503142376	28.850209846822473	21.337800123564627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	930.0
1	930.0
2	562.0
3	194.0
4	194.0
5	194.0
6	481.5
7	769.0
8	769.0
9	769.0
10	712.5
11	656.0
12	656.0
13	656.0
14	774.5
15	893.0
16	893.0
17	893.0
18	1589.0
19	2285.0
20	2285.0
21	2285.0
22	3756.5
23	5228.0
24	5228.0
25	5228.0
26	8124.5
27	11021.0
28	11021.0
29	11021.0
30	13516.5
31	16012.0
32	16012.0
33	16012.0
34	16886.0
35	17760.0
36	17760.0
37	17760.0
38	18327.0
39	18894.0
40	18894.0
41	18894.0
42	19323.5
43	19753.0
44	19753.0
45	19753.0
46	21877.0
47	24001.0
48	24001.0
49	24001.0
50	23004.0
51	22007.0
52	22007.0
53	22007.0
54	19193.5
55	16380.0
56	16380.0
57	16380.0
58	14247.0
59	12114.0
60	12114.0
61	12114.0
62	10222.0
63	8330.0
64	8330.0
65	8330.0
66	6783.0
67	5236.0
68	5236.0
69	5236.0
70	3984.5
71	2733.0
72	2733.0
73	2733.0
74	2162.5
75	1592.0
76	1592.0
77	1592.0
78	1122.5
79	653.0
80	653.0
81	653.0
82	455.0
83	257.0
84	257.0
85	257.0
86	175.0
87	93.0
88	93.0
89	93.0
90	64.0
91	35.0
92	35.0
93	35.0
94	24.5
95	14.0
96	14.0
97	14.0
98	40.5
99	67.0
100	67.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001064356303916299
2	0.0
3	5.321781519581495E-4
4	0.009579206735246691
5	0.030866332813572667
6	0.06279702193106164
7	0.10590345223967176
8	0.1074999866955462
9	0.11654701527883474
10	0.12772275646995587
11	0.13996285396499333
12	0.13198018168562106
13	0.12612622201408144
14	0.1170791934307929
15	0.13730196320520258
16	0.11335394636708584
17	0.12506186571016514
18	0.1074999866955462
19	0.09738860180834137
20	0.08514850431330392
21	0.08727721692113652
22	0.092598998440718
23	0.07716583203393168
24	0.08461632616134578
25	0.08035890094568057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	187907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.89302686967489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.90654970905346	29.127174612973437
2	15.506395860990832	13.302325086345906
3	6.214717304184916	7.997041089475113
4	3.1526445737540167	5.409058736502632
5	1.81391828682738	3.890222290814073
6	1.1104356133450788	2.857796676015263
7	0.7742031538852839	2.324554167753197
8	0.5583195821288105	1.9158413470493383
9	0.38710157694264197	1.4943562506984838
>10	2.317646620925818	18.269143778571316
>50	0.1612923237261008	4.679974668319967
>100	0.09181255350562662	6.9784521066272145
>500	0.0037221305475254036	1.099480061945537
>1k	0.0012407101825084679	0.6545791269085239
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1230	0.6545791269085239	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	803	0.427339056022394	No Hit
TATCAACGCAGAGTACTTTTTTTTT	732	0.3895544072333655	No Hit
GGTATCAACGCAGAGTACTTTTTTT	531	0.28258659868977737	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	456	0.2426732372929162	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	437	0.23256185240571134	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	370	0.1969059162245153	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	317	0.1687004741707334	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	313	0.1665717615629008	No Hit
CTGTAGGACCTGGAATATGGCGAGA	299	0.1591212674354867	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	280	0.14900988254828187	No Hit
GTATCAACGCAGAGTACATGGGGTG	273	0.14528463548457482	No Hit
GTGTATATCAATGAGTTACAATGAA	254	0.13517325059736998	No Hit
GTACATGGGGTGGTATCAACGCAAA	249	0.13251235983757922	No Hit
GTCCTACAGTGTGCATTTCTCATTT	247	0.13144800353366293	No Hit
ATAAATAATCCACCTATAACTTCTC	239	0.12719057831799774	No Hit
GTCCTACAGTGGACATTTCTAAATT	232	0.12346533125429067	No Hit
CTTTAGGACGTGAAATATGGCGAGG	230	0.12240097495037439	No Hit
CCTATAACTTCTCTGTTAACCCAAC	226	0.12027226234254179	No Hit
ACCTATAACTTCTCTGTTAACCCAA	216	0.1149504808229603	No Hit
CTGTAGGACGTGGAATATGGCAAGA	214	0.113886124519044	No Hit
CCATTGGGATGTCCTGATCCAACAT	214	0.113886124519044	No Hit
GTTATATAATTTAAGCTCCATAGGG	211	0.11228959006316953	No Hit
GTCAGGATACCGCGGCCGTTAAACT	210	0.11175741191121139	No Hit
GATATACACTGTTCTACAAATCCCG	207	0.11016087745533695	No Hit
GGTATCAACGCAGAGTACATGGGGT	206	0.10962869930337879	No Hit
GACTATAGGCAATAATCACACTATA	201	0.10696780854358805	No Hit
CTCTAATACTTGTAATGCTAGAGGT	200	0.1064356303916299	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	189	0.10058167072009025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.321781519581495E-4	0.0
9	0.0	0.0	0.0	0.001064356303916299	0.0
10	0.0	0.0	0.0	0.001064356303916299	0.0
11	0.0	0.0	0.0	0.001064356303916299	0.0
12	0.0	0.0	0.0	0.001064356303916299	0.0
13	0.0	0.0	0.0	0.001064356303916299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	30	7.6794554E-4	19.0032	12
AAGCGTT	30	7.6794554E-4	19.0032	11
AAAGCGT	30	7.6794554E-4	19.0032	10
AATCGTA	30	7.6794554E-4	19.0032	13
GAAAGCG	35	9.887911E-5	18.998133	9
TCAAGCT	35	9.887911E-5	18.998133	17
TTCAAGC	40	2.7413756E-4	16.627802	16
AAGAAAG	40	2.7469636E-4	16.623367	7
AAATCGT	35	0.0021582101	16.288458	12
GCGTTCA	35	0.0021582101	16.288458	13
GTGGCAG	35	0.0021620614	16.284115	17
TGTTGGA	35	0.0021620614	16.284115	2
TAGCATT	55	1.1188313E-5	15.543928	5
TATTACA	50	8.6562475E-5	15.198507	4
GTTCAAG	45	6.714589E-4	14.780267	15
AAGCTCA	45	6.714589E-4	14.780267	19
CCAATAA	45	6.755471E-4	14.76845	1
TAGCATA	60	2.5405401E-5	14.2524	19
TATCACC	40	0.0052596554	14.2486	3
GCATTAC	40	0.0052596554	14.2486	7
>>END_MODULE