FastQCFastQC Report
Thu 2 Feb 2017
SRR4061995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187907
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.2937623398808985No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG5120.27247521380257256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.15965344558744485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.15007423885219814No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT2780.14794552624436558No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC2600.13836631950911887No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.1367697850532444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.13623760690128628No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT2280.1213366186464581No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC2250.11974008419058364No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2200.1170791934307929No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2160.1149504808229603No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA2150.11441830267100214No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC2100.11175741191121139No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG2080.1106930556072951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAA250.002347964835.1993633
GTGTAAC702.7284841E-1134.570838
AGTGTAA702.7284841E-1134.570837
CTATGTA454.051995E-529.3328024
TCGTTCA300.00573334129.332833
TGTAGTA300.00573334129.33287
TTCGCTT300.00573334129.332836
TATGTAG300.00573334129.33285
ATTGAAC300.00573334129.33288
AGCTTAC407.0106465E-427.499515
TATATGG407.0106465E-427.49953
GTGAGGA407.0106465E-427.49959
GTTATAT756.947812E-826.3995231
AGGACCT1450.024.2754215
GGACCTG1400.023.5710016
CTGTAGG2550.023.2936941
TGTAGGA2300.022.9561062
TAGGACC1750.022.628164
CGTGATT703.197206E-521.99960128
GACGTGA904.0447048E-721.9996017
Produced by FastQC (version 0.11.5)