##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187907
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00927586518863	32.0	32.0	32.0	32.0	32.0
2	31.41568967627603	32.0	32.0	32.0	32.0	32.0
3	31.568887800880223	32.0	32.0	32.0	32.0	32.0
4	31.64903383056512	32.0	32.0	32.0	32.0	32.0
5	31.669985684407713	32.0	32.0	32.0	32.0	32.0
6	35.22861309051818	36.0	36.0	36.0	36.0	36.0
7	35.255126206048736	36.0	36.0	36.0	36.0	36.0
8	35.191557525797336	36.0	36.0	36.0	36.0	36.0
9	35.299068156055924	36.0	36.0	36.0	36.0	36.0
10	35.18524057113359	36.0	36.0	36.0	36.0	36.0
11	35.288743899907935	36.0	36.0	36.0	36.0	36.0
12	35.23942695056597	36.0	36.0	36.0	36.0	36.0
13	35.2657697690879	36.0	36.0	36.0	36.0	36.0
14	35.200955791960915	36.0	36.0	36.0	36.0	36.0
15	35.19553821837398	36.0	36.0	36.0	36.0	36.0
16	35.22137546765155	36.0	36.0	36.0	36.0	36.0
17	35.2172564087554	36.0	36.0	36.0	36.0	36.0
18	35.20940678101401	36.0	36.0	36.0	36.0	36.0
19	35.18129180924606	36.0	36.0	36.0	36.0	36.0
20	35.13547659214399	36.0	36.0	36.0	36.0	36.0
21	35.10378006141336	36.0	36.0	36.0	36.0	36.0
22	35.096840458311824	36.0	36.0	36.0	36.0	36.0
23	35.06396781386537	36.0	36.0	36.0	36.0	36.0
24	35.063845412890416	36.0	36.0	36.0	36.0	36.0
25	35.05727833449526	36.0	36.0	36.0	36.0	36.0
26	35.02430457619993	36.0	36.0	36.0	36.0	36.0
27	34.98631237793164	36.0	36.0	36.0	36.0	36.0
28	34.98084690831103	36.0	36.0	36.0	36.0	36.0
29	34.91386696610557	36.0	36.0	36.0	32.0	36.0
30	34.89904048279202	36.0	36.0	36.0	36.0	36.0
31	34.8827824402497	36.0	36.0	36.0	32.0	36.0
32	34.81512663179126	36.0	36.0	36.0	32.0	36.0
33	34.75562911440234	36.0	36.0	36.0	32.0	36.0
34	34.73099458774819	36.0	36.0	36.0	32.0	36.0
35	34.68428531135083	36.0	36.0	36.0	32.0	36.0
36	34.655223062472395	36.0	36.0	36.0	32.0	36.0
37	34.62517628401284	36.0	36.0	36.0	32.0	36.0
38	34.62358507133848	36.0	36.0	36.0	32.0	36.0
39	34.55095339715923	36.0	36.0	36.0	32.0	36.0
40	34.560878519693254	36.0	36.0	36.0	32.0	36.0
41	34.52635612297573	36.0	36.0	36.0	32.0	36.0
42	34.51379671858952	36.0	36.0	36.0	32.0	36.0
43	34.478688925904834	36.0	36.0	36.0	32.0	36.0
44	34.4125285380534	36.0	36.0	36.0	32.0	36.0
45	34.34624574922701	36.0	36.0	36.0	32.0	36.0
46	34.226649353137454	36.0	36.0	36.0	32.0	36.0
47	34.12984082551475	36.0	36.0	36.0	32.0	36.0
48	34.09394540916517	36.0	36.0	36.0	32.0	36.0
49	33.99531151048125	36.0	36.0	36.0	32.0	36.0
50	33.64013049008286	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	6.0
21	6.0
22	21.0
23	65.0
24	154.0
25	313.0
26	665.0
27	1251.0
28	2050.0
29	3050.0
30	4238.0
31	6016.0
32	8746.0
33	14147.0
34	30064.0
35	117112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15909163499313	21.091379475550163	13.301287169823372	24.44824171963333
2	14.450854746343644	21.67947544333979	40.93787920720413	22.931790603112425
3	17.65670069077092	26.974125360552613	30.21404319180867	25.155130756867795
4	10.627597694604246	18.31650763409559	39.244945638001774	31.810949033298385
5	11.547733719339886	40.298126200726955	34.2802556583842	13.873884421548958
6	28.576902403848713	40.220428190541064	18.352695748428744	12.84997365718148
7	27.257632765144457	33.58150574486315	20.824131086122392	18.33673040387
8	25.947410155023498	35.59739658448062	19.336693151399363	19.11850010909652
9	26.835615490641647	14.951545179264212	20.898103849244574	37.31473548084957
10	15.55290649097692	27.557781242848854	32.70766921934787	24.181643046826355
11	33.26645627890393	23.412113439094874	24.632930119686865	18.688500162314337
12	23.328561469237442	27.05274417664057	30.019637373807257	19.59905698031473
13	28.053239102321893	22.016742324660605	26.885108058773753	23.04491051424375
14	21.43986120793797	23.23383375818889	27.732867854843086	27.59343717903005
15	24.032633164278074	29.832310664317983	24.47008360518767	21.664972566216267
16	22.274967270869478	27.965578533947827	27.14814854235628	22.611305652826413
17	19.91942822779354	28.621605368613196	27.702001522029512	23.756964881563754
18	20.002554468723723	27.464264046917076	31.226251423584134	21.306930060775066
19	23.641482222588834	26.119303698105977	28.59127121395158	21.647942865353606
20	23.474378282873975	26.601457103780064	29.227224105541573	20.696940507804392
21	24.35619748066863	25.03206373365548	27.479018876359053	23.132719909316844
22	23.094935260527812	27.37843720563896	28.2363083865955	21.290319147237728
23	22.348940427660636	26.934211786744438	28.89157344629762	21.82527433929731
24	22.49328117931934	27.48197227322317	28.7714536600942	21.253292887363294
25	22.142986386810428	26.863963896842037	29.785637499600863	21.207412216746672
26	20.14954365237753	27.58574811739975	30.82568319097416	21.43902503924856
27	21.22679666212534	28.07018477520436	28.82056794959128	21.88245061307902
28	20.686315206863153	27.43238496663154	29.298783408372447	22.582516418132855
29	21.581118118198024	26.873153987387244	28.35794683483675	23.18778105957798
30	21.24831028940618	27.063043501399665	29.52815829528158	22.160487913912572
31	22.43580532716	26.159495489742156	29.015193847955086	22.389505335142758
32	20.84320101328877	27.91653140184031	29.267760493446087	21.97250709142483
33	21.405383542835246	27.4951305439954	29.27101848796739	21.828467425201964
34	21.143018317669473	27.60103455983311	30.148052749779147	21.107894372718274
35	21.881569074063233	27.87016981804829	28.613090518181867	21.63517058970661
36	20.384019754453	27.83717477262688	29.345367655276277	22.433437817643835
37	22.283187159400544	27.03454955722071	27.41240207765668	23.26986120572207
38	21.760764633568733	28.257595512673824	28.44758311292288	21.53405674083456
39	21.947260583805647	27.46600675873447	27.750725100449696	22.83600755701019
40	22.50687840261406	27.542880254594028	29.23733549042878	20.712905852363136
41	22.555546685825284	27.238764269178574	29.14185359623214	21.063835448764003
42	21.797232570516233	29.001064395955297	27.827035657264503	21.37466737626397
43	20.782220424585287	28.535239301547094	28.873715413967993	21.808824859899627
44	20.807544318079007	28.462557809082345	28.411999808411785	22.31789806442686
45	21.285677032054114	28.394207588038384	28.48468076274208	21.835434617165422
46	22.562293110238315	27.378633543017106	27.232812849251204	22.826260497493376
47	22.809398366195683	27.872063010563846	27.11529762379926	22.203240999441206
48	21.70356572645024	28.690792974986696	26.72112825971261	22.88451303885045
49	22.134410490574872	28.040680780406806	27.511149428957648	22.31375930006067
50	20.961656156036295	29.234985764082914	27.049839014395573	22.75351906548522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	575.0
1	303.5
2	32.0
3	96.0
4	160.0
5	143.0
6	126.0
7	93.5
8	61.0
9	94.0
10	127.0
11	233.0
12	339.0
13	578.0
14	817.0
15	1090.5
16	1364.0
17	1466.5
18	1569.0
19	1552.5
20	1536.0
21	1641.5
22	1747.0
23	1636.5
24	1526.0
25	2051.0
26	2576.0
27	3611.5
28	4647.0
29	5341.0
30	6035.0
31	6305.5
32	6576.0
33	7770.5
34	8965.0
35	9971.0
36	10977.0
37	12333.5
38	13690.0
39	13256.0
40	12822.0
41	12764.5
42	12707.0
43	11603.0
44	10499.0
45	10372.5
46	10246.0
47	10267.5
48	10289.0
49	10015.0
50	9741.0
51	9642.0
52	9543.0
53	9106.5
54	8670.0
55	8559.0
56	8448.0
57	8149.5
58	7851.0
59	7241.5
60	6632.0
61	5876.5
62	5121.0
63	4420.0
64	3719.0
65	3221.0
66	2723.0
67	2330.5
68	1938.0
69	1684.0
70	1430.0
71	1125.0
72	820.0
73	656.0
74	492.0
75	402.5
76	313.0
77	256.5
78	200.0
79	159.0
80	118.0
81	83.5
82	49.0
83	43.0
84	37.0
85	32.0
86	27.0
87	21.5
88	16.0
89	10.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028205442053781924
2	0.007982672279372242
3	5.321781519581495E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.321781519581495E-4
17	0.0
18	5.321781519581495E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	5.321781519581495E-4
24	0.001064356303916299
25	5.321781519581495E-4
26	0.001064356303916299
27	0.0015965344558744486
28	0.0026608907597907476
29	0.001064356303916299
30	0.0026608907597907476
31	0.001064356303916299
32	0.002128712607832598
33	5.321781519581495E-4
34	5.321781519581495E-4
35	0.0
36	0.0
37	0.0015965344558744486
38	0.0
39	0.001064356303916299
40	0.0
41	0.001064356303916299
42	0.0037252470637070467
43	0.003193068911748897
44	0.002128712607832598
45	0.003193068911748897
46	0.0026608907597907476
47	0.001064356303916299
48	0.0037252470637070467
49	0.0026608907597907476
50	0.001064356303916299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	187907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.7103407536707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.69274939616278	32.08661731601271
2	15.59722918470583	14.571037800614134
3	6.066855033495876	8.501545977531439
4	3.041972382992298	5.6836626629130365
5	1.7351775053547829	4.052536627161309
6	1.0743745157909128	3.0110639837792097
7	0.7337191815157453	2.399059109027338
8	0.48193045618192587	1.8008908662263778
9	0.3828100077473454	1.609306731521444
>10	1.9983593856810828	16.923265232269156
>50	0.1344392289112701	4.35694253008137
>100	0.058105090461650646	4.437833609179009
>500	0.0022786309984961033	0.5662375536834711
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	552	0.2937623398808985	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	512	0.27247521380257256	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	300	0.15965344558744485	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.15007423885219814	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	278	0.14794552624436558	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	260	0.13836631950911887	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.1367697850532444	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.13623760690128628	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	228	0.1213366186464581	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	225	0.11974008419058364	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	220	0.1170791934307929	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	216	0.1149504808229603	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	215	0.11441830267100214	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	210	0.11175741191121139	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	208	0.1106930556072951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.321781519581495E-4	0.0
3	0.0	0.0	0.0	5.321781519581495E-4	0.0
4	0.0	0.0	0.0	5.321781519581495E-4	0.0
5	0.0	0.0	0.0	5.321781519581495E-4	0.0
6	0.0	0.0	0.0	5.321781519581495E-4	0.0
7	0.0	0.0	0.0	5.321781519581495E-4	0.0
8	0.0	0.0	0.0	5.321781519581495E-4	0.0
9	0.0	0.0	0.0	5.321781519581495E-4	0.0
10	0.0	0.0	0.0	5.321781519581495E-4	0.0
11	0.0	0.0	0.0	5.321781519581495E-4	0.0
12	0.0	0.0	0.0	5.321781519581495E-4	0.0
13	0.0	0.0	0.0	5.321781519581495E-4	0.0
14	0.0	0.0	0.0	5.321781519581495E-4	0.0
15	0.0	0.0	0.0	0.001064356303916299	0.0
16	0.0	0.0	0.0	0.001064356303916299	0.0
17	0.0	0.0	0.0	0.001064356303916299	0.0
18	0.0	0.0	0.0	0.0015965344558744486	0.0
19	0.0	0.0	0.0	0.0015965344558744486	0.0
20	0.0	0.0	0.0	0.0015965344558744486	0.0
21	0.0	0.0	0.0	0.0015965344558744486	0.0
22	0.0	0.0	0.0	0.0037252470637070467	0.0
23	0.0	0.0	0.0	0.004789603367623346	0.0
24	0.0	0.0	0.0	0.006918315975455944	0.0
25	0.0	0.0	0.0	0.007450494127414093	0.0
26	0.0	0.0	0.0	0.009047028583288541	0.0
27	0.0	0.0	0.0	0.01064356303916299	0.0
28	0.0	0.0	0.0	0.015965344558744484	0.0
29	0.0	0.0	0.0	0.020754947926367832	0.0
30	0.0	0.0	0.0	0.027141085749865627	0.0
31	0.0	0.0	0.0	0.041509895852735665	0.0
32	0.0	0.0	0.0	0.0558787059556057	0.0
33	0.0	0.0	0.0	0.06492573453889423	0.0
34	0.0	0.0	0.0	0.07663365388197353	0.0
35	0.0	0.0	0.0	0.09100246398484356	0.0
36	0.0	0.0	0.0	0.1074999866955462	0.0
37	0.0	0.0	0.0	0.13464107244541182	0.0
38	0.0	0.0	0.0	0.15965344558744485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCATAA	25	0.0023479648	35.19936	33
GTGTAAC	70	2.7284841E-11	34.5708	38
AGTGTAA	70	2.7284841E-11	34.5708	37
CTATGTA	45	4.051995E-5	29.332802	4
TCGTTCA	30	0.005733341	29.3328	33
TGTAGTA	30	0.005733341	29.3328	7
TTCGCTT	30	0.005733341	29.3328	36
TATGTAG	30	0.005733341	29.3328	5
ATTGAAC	30	0.005733341	29.3328	8
AGCTTAC	40	7.0106465E-4	27.4995	15
TATATGG	40	7.0106465E-4	27.4995	3
GTGAGGA	40	7.0106465E-4	27.4995	9
GTTATAT	75	6.947812E-8	26.399523	1
AGGACCT	145	0.0	24.275421	5
GGACCTG	140	0.0	23.571001	6
CTGTAGG	255	0.0	23.293694	1
TGTAGGA	230	0.0	22.956106	2
TAGGACC	175	0.0	22.62816	4
CGTGATT	70	3.197206E-5	21.999601	28
GACGTGA	90	4.0447048E-7	21.999601	7
>>END_MODULE