FastQCFastQC Report
Thu 2 Feb 2017
SRR4061994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334066
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46850.14051911389876504No Hit
TATCAACGCAGAGTACTTTTTTTTT33670.10098780288092678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.014.2608291
GCGTAAC1750.012.48755111
CGCGTAA1750.012.48736210
TCGCGTA1800.012.14049159
CCTAGCG550.003070820812.0903263
GTATCAA36650.011.8679791
TAATACA5500.011.0551744
TAGGCCG700.001490348410.858745
CGACCAT4950.010.74889310
GCGGTCG2150.010.6060519
CGACCCG909.507471E-510.5571085
GTCTTAC1553.6870915E-910.4160371
CGTAACT2100.010.40566712
AAGACGG4950.010.1732125
ACGGTAT2350.010.1077179
TTAACCG856.588249E-410.0603035
TCGGCGT1803.947207E-1010.02789613
CGGTCCA2750.010.01950510
CGTCAAT3900.09.9847519
CCTAATA2950.09.9809212
Produced by FastQC (version 0.11.5)