##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061994_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3334066
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227346729188923	32.0	32.0	32.0	32.0	32.0
2	30.71602151847024	32.0	32.0	32.0	32.0	32.0
3	30.747435113761995	32.0	32.0	32.0	32.0	32.0
4	30.761437236095507	32.0	32.0	32.0	32.0	32.0
5	30.642812709766392	32.0	32.0	32.0	32.0	32.0
6	34.29938099605707	36.0	36.0	36.0	32.0	36.0
7	34.24212118176425	36.0	36.0	36.0	32.0	36.0
8	34.18719695410949	36.0	36.0	36.0	32.0	36.0
9	34.35028160810254	36.0	36.0	36.0	32.0	36.0
10	34.00137339812709	36.0	36.0	36.0	32.0	36.0
11	34.33191484511704	36.0	36.0	36.0	32.0	36.0
12	34.14191140787255	36.0	36.0	36.0	32.0	36.0
13	34.23030347929525	36.0	36.0	36.0	32.0	36.0
14	34.12910032374884	36.0	36.0	36.0	32.0	36.0
15	34.07293526882791	36.0	36.0	36.0	32.0	36.0
16	34.07599939533291	36.0	36.0	36.0	32.0	36.0
17	34.020832221077804	36.0	36.0	36.0	32.0	36.0
18	34.02092460077275	36.0	36.0	36.0	32.0	36.0
19	34.02085471613339	36.0	36.0	36.0	32.0	36.0
20	34.005723641943504	36.0	36.0	36.0	32.0	36.0
21	33.99239337193685	36.0	36.0	36.0	32.0	36.0
22	33.95833165870142	36.0	36.0	36.0	32.0	36.0
23	33.918628185524824	36.0	36.0	36.0	32.0	36.0
24	33.90078180815857	36.0	36.0	36.0	32.0	36.0
25	33.52641219459963	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	54.0
5	247.0
6	613.0
7	140.0
8	547.0
9	466.0
10	274.0
11	77.0
12	126.0
13	124.0
14	414.0
15	618.0
16	944.0
17	1437.0
18	2054.0
19	2927.0
20	4433.0
21	6478.0
22	9895.0
23	14802.0
24	21188.0
25	29810.0
26	41299.0
27	53150.0
28	70979.0
29	93671.0
30	123973.0
31	167252.0
32	234964.0
33	330427.0
34	700140.0
35	1420543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16619766637613	17.31183950292168	12.149277063696259	25.372685767005937
2	17.012300903641922	18.877382077848537	37.50191957922823	26.608397439281312
3	19.938003329284204	22.037551926335837	27.620251645896133	30.404193098483827
4	13.545346831646043	15.39233595800525	33.989291338582674	37.07302587176603
5	15.697437885310295	35.46726880182225	32.90536413135443	15.929929181513028
6	35.61794343675281	33.605754701324365	16.37412281109801	14.402179050824811
7	31.642746544990857	29.459025238403065	19.215762690134717	19.682465526471365
8	28.522551693035876	33.243727867610104	18.233396166251218	20.000324273102805
9	26.78166638690461	15.342481302235806	18.504353027480843	39.37149928337874
10	17.35462158425143	26.523729894730014	30.015370643332602	26.106277877685958
11	36.71754720981962	21.64530508803429	21.452453192643457	20.184694509502634
12	24.72107889437653	23.297589426090298	28.005333805847666	23.975997873685507
13	29.69929994116875	19.57972445112607	24.69335825527957	26.027617352425608
14	23.89326455053464	19.14205669776506	24.767152410150466	32.19752634154984
15	25.610061185563776	26.662075672123702	21.75605841595071	25.971804726361807
16	26.0358199417935	25.902164787106646	22.980038508058946	25.081976763040913
17	24.364010918886127	25.958000126125746	24.61049666517518	25.067492289812943
18	24.994460141544135	25.41590975285325	25.10468681033266	24.48494329526995
19	25.77591828255014	25.011498421443868	24.849079465800752	24.363503830205243
20	26.063927033852995	24.40866758188563	24.729374475958092	24.798030908303286
21	26.400893641957225	24.26984816972713	24.511841176216297	24.817417012099348
22	26.234775100218332	23.98244467876092	24.653078846426364	25.129701374594383
23	25.117135051784363	24.466659922777463	24.852922680509852	25.563282344928318
24	24.882179514620727	24.598161523112147	24.975481271053308	25.544177691213815
25	25.217645552573998	24.37591164075373	24.94438222164319	25.462060585029082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	145.0
2	444.5
3	744.0
4	744.0
5	744.0
6	1551.5
7	2359.0
8	2359.0
9	2359.0
10	2898.0
11	3437.0
12	3437.0
13	3437.0
14	4806.0
15	6175.0
16	6175.0
17	6175.0
18	11355.5
19	16536.0
20	16536.0
21	16536.0
22	26558.5
23	36581.0
24	36581.0
25	36581.0
26	54647.5
27	72714.0
28	72714.0
29	72714.0
30	95417.0
31	118120.0
32	118120.0
33	118120.0
34	150255.0
35	182390.0
36	182390.0
37	182390.0
38	223191.0
39	263992.0
40	263992.0
41	263992.0
42	308921.5
43	353851.0
44	353851.0
45	353851.0
46	393420.0
47	432989.0
48	432989.0
49	432989.0
50	453760.0
51	474531.0
52	474531.0
53	474531.0
54	451019.0
55	427507.0
56	427507.0
57	427507.0
58	390826.5
59	354146.0
60	354146.0
61	354146.0
62	308037.5
63	261929.0
64	261929.0
65	261929.0
66	213433.0
67	164937.0
68	164937.0
69	164937.0
70	125246.5
71	85556.0
72	85556.0
73	85556.0
74	63299.0
75	41042.0
76	41042.0
77	41042.0
78	30838.0
79	20634.0
80	20634.0
81	20634.0
82	14293.0
83	7952.0
84	7952.0
85	7952.0
86	5563.5
87	3175.0
88	3175.0
89	3175.0
90	2117.0
91	1059.0
92	1059.0
93	1059.0
94	698.5
95	338.0
96	338.0
97	338.0
98	782.5
99	1227.0
100	1227.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.198417787770248E-4
2	5.99868148980854E-5
3	4.798945191846832E-4
4	0.009477916753897492
5	0.031133156932106325
6	0.05917699289696125
7	0.10398714362583104
8	0.10617666236961117
9	0.11562458571605962
10	0.1302313751437434
11	0.13700988522722704
12	0.13080124988527522
13	0.12606229150832646
14	0.11325510652758523
15	0.13074126307037715
16	0.11559459230861056
17	0.12165326061331719
18	0.10965589763370011
19	0.09519907524326153
20	0.08959030805029054
21	0.08932036738324917
22	0.10011799406490453
23	0.07963249677720836
24	0.0881506244927365
25	0.08539123100742456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3334066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.378790451323574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.75252393254794	30.06775062150831
2	15.994441845191362	14.196279660672575
3	6.36347932885749	8.472105470300873
4	3.184117052192248	5.652290537269036
5	1.7554306727835065	3.8951944989642597
6	1.0972417114582074	2.9216555992353253
7	0.7364301751163074	2.287731629646269
8	0.5192303333174092	1.8434251326611362
9	0.3864345701839145	1.5434548932005314
>10	1.989844455990383	15.897810372634291
>50	0.13986375872376466	4.268111319487523
>100	0.07397233533703329	5.973167059646042
>500	0.004750368748952559	1.423366828099353
>1k	0.0022394595514619677	1.5576563766745894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4685	0.14051911389876504	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3367	0.10098780288092678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.99934074490427E-5
2	0.0	0.0	0.0	0.0	2.99934074490427E-5
3	0.0	0.0	0.0	0.0	2.99934074490427E-5
4	0.0	2.99934074490427E-5	0.0	2.99934074490427E-5	2.99934074490427E-5
5	5.99868148980854E-5	2.99934074490427E-5	0.0	2.99934074490427E-5	2.99934074490427E-5
6	5.99868148980854E-5	2.99934074490427E-5	0.0	2.99934074490427E-5	2.99934074490427E-5
7	5.99868148980854E-5	2.99934074490427E-5	0.0	2.99934074490427E-5	5.99868148980854E-5
8	5.99868148980854E-5	2.99934074490427E-5	0.0	2.99934074490427E-5	8.99802223471281E-5
9	5.99868148980854E-5	2.99934074490427E-5	0.0	2.99934074490427E-5	8.99802223471281E-5
10	5.99868148980854E-5	2.99934074490427E-5	0.0	8.99802223471281E-5	8.99802223471281E-5
11	5.99868148980854E-5	2.99934074490427E-5	0.0	8.99802223471281E-5	1.199736297961708E-4
12	5.99868148980854E-5	5.99868148980854E-5	0.0	8.99802223471281E-5	6.298615564298967E-4
13	5.99868148980854E-5	5.99868148980854E-5	0.0	1.199736297961708E-4	8.99802223471281E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1545	0.0	14.260829	1
GCGTAAC	175	0.0	12.487551	11
CGCGTAA	175	0.0	12.487362	10
TCGCGTA	180	0.0	12.1404915	9
CCTAGCG	55	0.0030708208	12.090326	3
GTATCAA	3665	0.0	11.867979	1
TAATACA	550	0.0	11.055174	4
TAGGCCG	70	0.0014903484	10.85874	5
CGACCAT	495	0.0	10.748893	10
GCGGTCG	215	0.0	10.606051	9
CGACCCG	90	9.507471E-5	10.557108	5
GTCTTAC	155	3.6870915E-9	10.416037	1
CGTAACT	210	0.0	10.405667	12
AAGACGG	495	0.0	10.173212	5
ACGGTAT	235	0.0	10.107717	9
TTAACCG	85	6.588249E-4	10.060303	5
TCGGCGT	180	3.947207E-10	10.027896	13
CGGTCCA	275	0.0	10.019505	10
CGTCAAT	390	0.0	9.98475	19
CCTAATA	295	0.0	9.980921	2
>>END_MODULE