FastQCFastQC Report
Thu 2 Feb 2017
SRR4061994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334066
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.026.8740791
GTATCAA37150.024.703451
TACCGTA2700.018.7399547
TATGCGA2800.017.28498812
GGCGTTA7950.016.32709342
GTATAGG5600.015.7199931
CGTACAC3500.015.7138623
TATCAAC57800.015.5684562
TCAACGC57500.015.4952354
CGTCGTA5600.015.320785510
ATCAACG58900.015.2016283
ACCGTCG5500.015.1993628
CAACGCA58800.015.1900675
GTCCTAA2900.015.1779231
CGTTATT7900.015.03779544
ATACCGT6650.014.8865946
ACCGTAT3400.014.8817298
CTAGCGG3550.014.87350829
CCGTCGT5650.014.7958399
AACGCAG61000.014.7502016
Produced by FastQC (version 0.11.5)