##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3334066
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240543528532427	32.0	32.0	32.0	32.0	32.0
2	31.3763947684299	32.0	32.0	32.0	32.0	32.0
3	31.531385701422828	32.0	32.0	32.0	32.0	32.0
4	31.621010801825758	32.0	32.0	32.0	32.0	32.0
5	31.586131468303268	32.0	32.0	32.0	32.0	32.0
6	35.21935198643338	36.0	36.0	36.0	36.0	36.0
7	35.23078007453962	36.0	36.0	36.0	36.0	36.0
8	35.17471519759957	36.0	36.0	36.0	36.0	36.0
9	35.27783583168419	36.0	36.0	36.0	36.0	36.0
10	35.14014329650343	36.0	36.0	36.0	36.0	36.0
11	35.26985878503905	36.0	36.0	36.0	36.0	36.0
12	35.19522708908582	36.0	36.0	36.0	36.0	36.0
13	35.230412055430214	36.0	36.0	36.0	36.0	36.0
14	35.19272803837716	36.0	36.0	36.0	36.0	36.0
15	35.160779060762444	36.0	36.0	36.0	36.0	36.0
16	35.181425922582214	36.0	36.0	36.0	36.0	36.0
17	35.16685632497977	36.0	36.0	36.0	36.0	36.0
18	35.163609838557484	36.0	36.0	36.0	36.0	36.0
19	35.15242349731529	36.0	36.0	36.0	36.0	36.0
20	35.15241719869972	36.0	36.0	36.0	36.0	36.0
21	35.142652245036544	36.0	36.0	36.0	36.0	36.0
22	35.12598040950599	36.0	36.0	36.0	36.0	36.0
23	35.0926013462241	36.0	36.0	36.0	36.0	36.0
24	35.082320805886866	36.0	36.0	36.0	36.0	36.0
25	35.06952891754393	36.0	36.0	36.0	36.0	36.0
26	35.02698146947301	36.0	36.0	36.0	36.0	36.0
27	35.0138293603066	36.0	36.0	36.0	36.0	36.0
28	34.99510267643172	36.0	36.0	36.0	36.0	36.0
29	34.97654125623188	36.0	36.0	36.0	36.0	36.0
30	34.95664452953241	36.0	36.0	36.0	36.0	36.0
31	34.95828306938135	36.0	36.0	36.0	36.0	36.0
32	34.93759031764818	36.0	36.0	36.0	36.0	36.0
33	34.918332750461445	36.0	36.0	36.0	32.0	36.0
34	34.906658416480056	36.0	36.0	36.0	32.0	36.0
35	34.89816638302901	36.0	36.0	36.0	32.0	36.0
36	34.874712738140154	36.0	36.0	36.0	32.0	36.0
37	34.86313648260112	36.0	36.0	36.0	32.0	36.0
38	34.852549709573836	36.0	36.0	36.0	32.0	36.0
39	34.83573510542383	36.0	36.0	36.0	32.0	36.0
40	34.82245102526465	36.0	36.0	36.0	32.0	36.0
41	34.80131317136493	36.0	36.0	36.0	32.0	36.0
42	34.77599183699423	36.0	36.0	36.0	32.0	36.0
43	34.76618549242876	36.0	36.0	36.0	32.0	36.0
44	34.731462124625004	36.0	36.0	36.0	32.0	36.0
45	34.718113858573886	36.0	36.0	36.0	32.0	36.0
46	34.70626646263151	36.0	36.0	36.0	32.0	36.0
47	34.68020849017386	36.0	36.0	36.0	32.0	36.0
48	34.65183952567226	36.0	36.0	36.0	32.0	36.0
49	34.63990214950754	36.0	36.0	36.0	32.0	36.0
50	34.219542144636605	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	5.0
20	34.0
21	70.0
22	237.0
23	602.0
24	1474.0
25	3613.0
26	7564.0
27	14609.0
28	25562.0
29	41069.0
30	62352.0
31	93359.0
32	143477.0
33	248397.0
34	568594.0
35	2123043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.886298326031685	17.777445081634234	12.58110468587517	25.755151906458913
2	16.463348243650966	19.361876798363976	37.179751362729846	26.995023595255212
3	19.308404975193916	22.434772489165617	27.997838071948895	30.258984463691576
4	13.298125050988824	15.833979191761166	34.12403001262123	36.74386574462878
5	15.427902832070059	35.79968344929295	32.681206083988215	16.091207634648775
6	35.80510374585228	33.77388716043288	16.265299285166048	14.155709808548794
7	31.78773305627423	29.390539959316943	19.24398017315794	19.577746811250886
8	28.624358366031146	33.2424733043677	18.19622047074053	19.936947858860623
9	26.841340273407905	15.163017168826293	18.501703325609032	39.49393923215677
10	17.343463515836383	26.50351837982666	30.148650460414217	26.004367643922745
11	36.98151146378026	21.465291928834045	21.443156794136648	20.11003981324905
12	24.757495729687335	23.18230148482408	28.02167924140651	24.038523544082075
13	29.883721558001554	19.39124780373274	24.75505883806739	25.969971800198316
14	23.92640097706524	18.936757700657395	24.747260552130644	32.38958077014672
15	25.610140891032152	26.6771263676244	21.746960018188002	25.96577272315545
16	26.169552142826717	25.768418867840776	23.040268693908377	25.021760295424126
17	24.557552250015448	25.84789863188071	24.64696259762104	24.947586520482798
18	25.218892018869465	25.30641283430672	25.08374164383167	24.390953502992144
19	25.886987806176542	24.879718901701075	24.834218888953874	24.399074403168505
20	26.202355747153845	24.28415333981792	24.716089307357787	24.797401605670448
21	26.52883896119633	24.15204138130439	24.49573583726297	24.823383820236312
22	26.26246750964138	23.87511225032738	24.73226384840612	25.130156391625118
23	25.21940190710196	24.326677592271775	24.880596173318807	25.573324327307457
24	25.077076506624874	24.458784802153552	24.995883354204	25.46825533701757
25	25.363430772091682	24.20037431839859	25.04163092445847	25.39456398505126
26	25.114365342832084	24.755732664037634	25.099878466202686	25.030023526927593
27	25.484050348314696	24.70862799462756	24.729683442439747	25.077638214618
28	25.457908582949635	24.269612302516755	25.016789056564498	25.25569005796911
29	25.12998428041272	24.63467181274945	25.001012280233958	25.23433162660386
30	25.271158327909465	24.674223087374738	24.89161765191075	25.163000932805048
31	25.500171562805185	24.413027255690967	24.6263710027666	25.460430178737248
32	25.61594867974148	24.610173111092713	24.43051141496633	25.34336679419948
33	25.313521652279803	24.46902575238598	24.677060400832616	25.5403921945016
34	25.666369529303225	24.43242964383108	24.8661082243863	25.0350926024794
35	25.66402444584147	24.501119354637463	24.93119508203802	24.903661117483047
36	25.153341479552573	24.686853453834992	24.858475937160136	25.3013291294523
37	25.939273200412476	24.455056277508135	24.61114440431264	24.994526117766753
38	25.34829844400201	24.330682116070886	24.900376897158004	25.4206425427691
39	25.729384495225617	24.521871936903054	24.538518387879705	25.210225179991625
40	25.808338297248223	24.475549644847636	24.542824938468453	25.173287119435688
41	25.10716026424517	24.734517587600575	25.02842660148019	25.129895546674064
42	25.820236622638365	24.544769574278416	24.693093647230462	24.941900155852757
43	25.651499163448737	24.078488844683417	24.774125160546454	25.49588683132139
44	25.155702596343044	24.671227863895954	24.74006574842828	25.433003791332727
45	25.44532910217322	24.670282948834657	24.606605588790625	25.2777823602015
46	25.40438819947408	24.548865967383797	24.62898025697161	25.417765576170513
47	25.44455315531811	24.711864528788354	24.741888325871876	25.101693990021662
48	25.694474647434866	24.811280884411545	24.251099980413464	25.24314448774012
49	25.439285313644106	24.841223802002315	24.41149175539557	25.30799912895801
50	25.469208061614466	24.92790989381594	24.37275458890193	25.230127455667663
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	16.5
2	28.0
3	104.0
4	180.0
5	215.0
6	250.0
7	228.0
8	206.0
9	276.0
10	346.0
11	589.5
12	833.0
13	1613.5
14	2394.0
15	3066.5
16	3739.0
17	3787.0
18	3835.0
19	3786.5
20	3738.0
21	4438.0
22	5138.0
23	6989.0
24	8840.0
25	11353.0
26	13866.0
27	17972.5
28	22079.0
29	26982.5
30	31886.0
31	38190.5
32	44495.0
33	52419.0
34	60343.0
35	70480.5
36	80618.0
37	93845.0
38	107072.0
39	122260.0
40	137448.0
41	151251.5
42	165055.0
43	174321.0
44	183587.0
45	200796.0
46	218005.0
47	231105.0
48	244205.0
49	255093.5
50	265982.0
51	267816.5
52	269651.0
53	270469.0
54	271287.0
55	267300.5
56	263314.0
57	250396.0
58	237478.0
59	218589.0
60	199700.0
61	176596.0
62	153492.0
63	133172.0
64	112852.0
65	94436.0
66	76020.0
67	64541.0
68	53062.0
69	44546.0
70	36030.0
71	28250.5
72	20471.0
73	17371.0
74	14271.0
75	11363.5
76	8456.0
77	7032.0
78	5608.0
79	4695.0
80	3782.0
81	2940.0
82	2098.0
83	1670.5
84	1243.0
85	928.5
86	614.0
87	415.0
88	216.0
89	149.0
90	82.0
91	61.5
92	41.0
93	34.5
94	28.0
95	36.0
96	44.0
97	42.0
98	40.0
99	26.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030833222857615895
2	0.00398912319072268
3	1.4996703724521351E-4
4	5.99868148980854E-5
5	8.99802223471281E-5
6	1.4996703724521351E-4
7	0.0
8	0.0
9	0.0
10	8.99802223471281E-5
11	0.0
12	2.99934074490427E-5
13	0.0
14	0.0
15	0.0
16	3.599208893885124E-4
17	0.0
18	5.99868148980854E-5
19	2.99934074490427E-5
20	2.099538521432989E-4
21	0.0
22	0.0
23	5.99868148980854E-5
24	0.0012297297054107508
25	1.799604446942562E-4
26	4.199077042865978E-4
27	3.599208893885124E-4
28	0.0014696769650030925
29	2.699406670413843E-4
30	0.0010797626681655372
31	2.9993407449042703E-4
32	6.598549638789394E-4
33	1.799604446942562E-4
34	8.998022234712811E-4
35	5.99868148980854E-5
36	1.199736297961708E-4
37	0.0015596571873502205
38	0.0
39	6.598549638789394E-4
40	1.199736297961708E-4
41	0.0012297297054107508
42	0.004499011117356405
43	0.004199077042865978
44	0.004379037487560234
45	0.0021295319288820317
46	0.002369479188474373
47	0.0013197099277578788
48	0.0042890572652131064
49	0.003839156153477466
50	0.0013197099277578788
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3334066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.636897608932046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.67295508313714	29.76073869330918
2	16.42964825405701	14.66737053734224
3	6.738223559432393	9.023201852654323
4	3.2476592214231035	5.798617285414681
5	1.9104160627012854	4.263752309062819
6	1.1415165558900249	3.057225457449828
7	0.7673198015508333	2.397554279059142
8	0.5293275334576414	1.8902031130029835
9	0.3930138167141048	1.5788625746006775
>10	1.9567085733611913	15.782608444046552
>50	0.1395018446417223	4.279103484345913
>100	0.0684406517207671	5.491634998586785
>500	0.0037829018879540514	1.115963879375554
>1k	0.0014861400248208	0.8931630917491811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.799604446942562E-4
2	0.0	0.0	0.0	0.0	1.799604446942562E-4
3	0.0	0.0	0.0	2.99934074490427E-5	1.799604446942562E-4
4	0.0	0.0	0.0	2.99934074490427E-5	1.799604446942562E-4
5	0.0	0.0	0.0	2.99934074490427E-5	1.799604446942562E-4
6	0.0	0.0	0.0	2.99934074490427E-5	2.399472595923416E-4
7	0.0	0.0	0.0	2.99934074490427E-5	2.399472595923416E-4
8	0.0	0.0	0.0	2.99934074490427E-5	2.399472595923416E-4
9	0.0	0.0	0.0	2.99934074490427E-5	2.399472595923416E-4
10	0.0	0.0	0.0	2.99934074490427E-5	2.699406670413843E-4
11	0.0	0.0	0.0	8.99802223471281E-5	3.299274819394697E-4
12	0.0	0.0	0.0	8.99802223471281E-5	8.698088160222383E-4
13	0.0	0.0	0.0	8.99802223471281E-5	0.0010797626681655372
14	0.0	2.99934074490427E-5	0.0	1.4996703724521351E-4	0.0011397494830636225
15	0.0	2.99934074490427E-5	0.0	2.9993407449042703E-4	0.0014396835575540496
16	0.0	2.99934074490427E-5	0.0	4.199077042865978E-4	0.0014396835575540496
17	0.0	2.99934074490427E-5	0.0	5.398813340827686E-4	0.0014396835575540496
18	0.0	2.99934074490427E-5	0.0	6.898483713279821E-4	0.001499670372452135
19	0.0	5.99868148980854E-5	0.0	8.098220011241529E-4	0.001499670372452135
20	0.0	5.99868148980854E-5	0.0	9.597890383693664E-4	0.0018595912618406473
21	0.0	5.99868148980854E-5	0.0	0.0010497692607164945	0.002009558299085861
22	0.0	5.99868148980854E-5	0.0	0.0011997362979617081	0.0020395517065349036
23	0.0	5.99868148980854E-5	0.0	0.0014396835575540496	0.0020695451139839463
24	0.0	5.99868148980854E-5	0.0	0.0018595912618406473	0.002099538521432989
25	0.0	5.99868148980854E-5	0.0	0.0024894528182705443	0.0021295319288820317
26	0.0	5.99868148980854E-5	0.0	0.0030593275598023557	0.0022495055586782024
27	0.0	5.99868148980854E-5	0.0	0.0037191825236812946	0.002369479188474373
28	0.0	5.99868148980854E-5	0.0	0.004379037487560234	0.0024594594108215016
29	0.0	5.99868148980854E-5	0.0	0.00521885289613343	0.0024594594108215016
30	0.0	5.99868148980854E-5	0.0	0.00632860897174801	0.0024594594108215016
31	0.0	5.99868148980854E-5	0.0	0.007798285936751102	0.0025494396331686296
32	0.0	5.99868148980854E-5	0.0	0.010017798087980262	0.002609426448066715
33	0.0	5.99868148980854E-5	0.0	0.012237310239209423	0.0026394198555157577
34	0.0	5.99868148980854E-5	0.0	0.014786749872378052	0.002789386892760971
35	0.0	1.199736297961708E-4	0.0	0.017816084024731366	0.002789386892760971
36	0.0	1.199736297961708E-4	0.0	0.02099538521432989	0.002789386892760971
37	0.0	1.199736297961708E-4	0.0	0.026694132629648003	0.002879367115108099
38	0.0	1.199736297961708E-4	0.0	0.03422247789935772	0.0029393539300061845
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1990	0.0	26.874079	1
GTATCAA	3715	0.0	24.70345	1
TACCGTA	270	0.0	18.739954	7
TATGCGA	280	0.0	17.284988	12
GGCGTTA	795	0.0	16.327093	42
GTATAGG	560	0.0	15.719993	1
CGTACAC	350	0.0	15.713862	3
TATCAAC	5780	0.0	15.568456	2
TCAACGC	5750	0.0	15.495235	4
CGTCGTA	560	0.0	15.3207855	10
ATCAACG	5890	0.0	15.201628	3
ACCGTCG	550	0.0	15.199362	8
CAACGCA	5880	0.0	15.190067	5
GTCCTAA	290	0.0	15.177923	1
CGTTATT	790	0.0	15.037795	44
ATACCGT	665	0.0	14.886594	6
ACCGTAT	340	0.0	14.881729	8
CTAGCGG	355	0.0	14.873508	29
CCGTCGT	565	0.0	14.795839	9
AACGCAG	6100	0.0	14.750201	6
>>END_MODULE